##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4063998_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	814956
Sequences flagged as poor quality	0
Sequence length	50
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.322046834430324	32.0	32.0	32.0	32.0	32.0
2	31.416442114666314	32.0	32.0	32.0	32.0	32.0
3	31.490116030804117	32.0	32.0	32.0	32.0	32.0
4	31.5773587776518	32.0	32.0	32.0	32.0	32.0
5	31.52527989241137	32.0	32.0	32.0	32.0	32.0
6	35.10794815916442	36.0	36.0	36.0	36.0	36.0
7	35.132327389454154	36.0	36.0	36.0	36.0	36.0
8	35.07936133975331	36.0	36.0	36.0	36.0	36.0
9	35.194339326294916	36.0	36.0	36.0	36.0	36.0
10	35.04403427915127	36.0	36.0	36.0	36.0	36.0
11	35.18785185948689	36.0	36.0	36.0	36.0	36.0
12	35.10479093349825	36.0	36.0	36.0	36.0	36.0
13	35.133753233303395	36.0	36.0	36.0	36.0	36.0
14	35.08731882457458	36.0	36.0	36.0	36.0	36.0
15	35.064424337019425	36.0	36.0	36.0	36.0	36.0
16	35.07364692081536	36.0	36.0	36.0	36.0	36.0
17	35.03494912608779	36.0	36.0	36.0	36.0	36.0
18	35.031939147634965	36.0	36.0	36.0	36.0	36.0
19	35.035527071400175	36.0	36.0	36.0	36.0	36.0
20	35.01977039251199	36.0	36.0	36.0	36.0	36.0
21	35.017413210038335	36.0	36.0	36.0	36.0	36.0
22	35.001474926253685	36.0	36.0	36.0	36.0	36.0
23	34.945887876155275	36.0	36.0	36.0	36.0	36.0
24	34.927337181393845	36.0	36.0	36.0	36.0	36.0
25	34.89034009198043	36.0	36.0	36.0	32.0	36.0
26	34.84421858357997	36.0	36.0	36.0	32.0	36.0
27	34.841397572384274	36.0	36.0	36.0	32.0	36.0
28	34.810518604685406	36.0	36.0	36.0	32.0	36.0
29	34.783517146937996	36.0	36.0	36.0	32.0	36.0
30	34.77402338285748	36.0	36.0	36.0	32.0	36.0
31	34.76275406279603	36.0	36.0	36.0	32.0	36.0
32	34.714940438502204	36.0	36.0	36.0	32.0	36.0
33	34.69107411933896	36.0	36.0	36.0	32.0	36.0
34	34.67421799459112	36.0	36.0	36.0	32.0	36.0
35	34.64198189840924	36.0	36.0	36.0	32.0	36.0
36	34.60848438443303	36.0	36.0	36.0	32.0	36.0
37	34.595179371647056	36.0	36.0	36.0	32.0	36.0
38	34.55967193320866	36.0	36.0	36.0	32.0	36.0
39	34.55124571142491	36.0	36.0	36.0	32.0	36.0
40	34.53930150830229	36.0	36.0	36.0	32.0	36.0
41	34.51511615351013	36.0	36.0	36.0	32.0	36.0
42	34.459494745728605	36.0	36.0	36.0	32.0	36.0
43	34.444006793004775	36.0	36.0	36.0	32.0	36.0
44	34.375965082777476	36.0	36.0	36.0	32.0	36.0
45	34.34177059865809	36.0	36.0	36.0	32.0	36.0
46	34.310367676291726	36.0	36.0	36.0	32.0	36.0
47	34.27728000039266	36.0	36.0	36.0	32.0	36.0
48	34.22794850274125	36.0	36.0	36.0	32.0	36.0
49	34.20345024762073	36.0	36.0	36.0	32.0	36.0
50	33.61922484158654	36.0	36.0	36.0	27.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	1.0
18	1.0
19	3.0
20	5.0
21	26.0
22	74.0
23	274.0
24	672.0
25	1460.0
26	2951.0
27	5321.0
28	9227.0
29	13918.0
30	20151.0
31	28509.0
32	41168.0
33	66959.0
34	140225.0
35	484011.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.988518470340196	17.836377157695544	11.86207929078606	26.313025081178193
2	15.910159015695422	20.541480408024533	37.16751068469147	26.380849891588575
3	17.98700541747704	24.634243608542803	28.985526808228673	28.393224165751484
4	12.123967428916407	16.197561586146982	36.170296310475656	35.50817467446095
5	14.129351768684446	37.18593396453305	33.52180976641684	15.162904500365665
6	34.012022779227756	36.013242481468254	16.75679456361287	13.217940175691115
7	29.91714869796332	30.705291341597196	20.978239262584143	18.39932069785534
8	28.116241858456164	33.18633153200909	19.345338889461516	19.35208772007323
9	27.665670689433032	13.665584794065857	18.14539631508875	40.52334820141236
10	15.50696361739984	27.178231793361558	32.05362292165164	25.261181667586968
11	37.173540657409724	20.8028408895695	22.758529294833096	19.26508915818768
12	24.672865774348555	23.55489130701535	29.23041243944458	22.541830479191518
13	30.033756547923623	19.439035134541502	25.07015742012116	25.457050897413712
14	23.679712376757	19.369413035075556	25.533925186053217	31.416949402114223
15	25.239993766236317	27.161293726845802	22.435045532244388	25.163666974673493
16	25.637646481379228	25.558991349162525	23.926253144364683	24.877109025093564
17	24.001050363455203	25.69868802732908	25.379406986389448	24.920854622826262
18	24.66605477118954	24.95441460499611	26.332283785342486	24.047246838471864
19	25.715684566245457	24.60678762236887	25.54598173639248	24.13154607499319
20	25.919167947932937	24.174641603339595	25.104147621507604	24.802042827219864
21	26.809545546017212	24.10682294504243	24.671270596203968	24.412360912736393
22	25.8950176451244	24.225970481841966	25.018528607679436	24.8604832653542
23	24.795324364253915	24.00742133240403	25.500277318307425	25.696976985034624
24	24.945517996240252	24.73041224716178	25.32480599989202	24.99926375670595
25	25.173631562414105	24.33639612046963	25.429231947225823	25.060740369890443
26	24.9660705389201	25.020185881908297	25.542441890429313	24.47130168874229
27	25.224473467524515	24.537278486992996	25.021628234878335	25.216619810604158
28	24.672792376240295	24.70788758733922	25.642941813121684	24.976378223298802
29	24.72764531922987	24.580025278244758	25.451756592590772	25.240572809934598
30	24.666902270881035	24.927416722499956	25.670428971464894	24.73525203515411
31	25.331021398892133	24.545528173939935	24.871702364210666	25.25174806295726
32	24.92895147594412	24.923920406019615	24.73715727370002	25.409970844336243
33	24.607323418249642	24.51173121287979	25.336842880282727	25.54410248858784
34	25.276280042310173	24.45645905067159	25.385123212431925	24.88213769458631
35	25.72355552719607	24.450814421781395	25.41948182306788	24.406148227954656
36	24.94287572555805	25.018346034434096	24.976868043539618	25.061910196468233
37	25.57063673500441	24.883577957672074	24.842224357393274	24.70356094993024
38	24.885199792856493	24.93048175590936	25.18990199952387	24.994416451710283
39	25.453753412890755	24.54520354633862	24.868546185231768	25.132496855538854
40	25.61517176216508	24.82823783491935	25.157343837238557	24.399246565677018
41	24.83888305339315	25.05632344135	25.49672612327839	24.60806738197846
42	25.762906117360355	25.15059335438184	25.013896677436335	24.07260385082147
43	24.95481290148415	24.542392431391075	25.304775168876425	25.198019498248346
44	24.97993741947359	24.947297380207374	24.736486901036873	25.336278299282167
45	24.90999403641468	25.2394637190685	24.823239822025016	25.027302422491797
46	24.84680041720351	24.971347935456162	24.763114301490887	25.41873734584944
47	25.10049868946764	24.88600331805197	25.137188687209793	24.876309305270595
48	25.51657113827872	25.16047173967195	24.49023917068843	24.832717951360898
49	25.007638458565197	25.32385223724009	24.59571387377217	25.07279543042254
50	25.01690298118278	25.502702636374234	24.544846584738846	24.93554779770414
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	79.0
1	56.0
2	33.0
3	89.5
4	146.0
5	158.0
6	170.0
7	164.5
8	159.0
9	211.0
10	263.0
11	403.0
12	543.0
13	998.0
14	1453.0
15	1907.5
16	2362.0
17	2488.0
18	2614.0
19	2609.5
20	2605.0
21	2797.5
22	2990.0
23	3504.5
24	4019.0
25	4356.0
26	4693.0
27	5982.5
28	7272.0
29	8497.5
30	9723.0
31	11085.0
32	12447.0
33	14636.0
34	16825.0
35	19534.0
36	22243.0
37	25782.5
38	29322.0
39	31751.5
40	34181.0
41	37445.0
42	40709.0
43	42116.5
44	43524.0
45	47852.0
46	52180.0
47	55843.0
48	59506.0
49	61326.5
50	63147.0
51	62328.0
52	61509.0
53	61035.5
54	60562.0
55	60443.0
56	60324.0
57	58610.5
58	56897.0
59	51833.0
60	46769.0
61	41059.5
62	35350.0
63	30673.0
64	25996.0
65	21905.5
66	17815.0
67	15128.0
68	12441.0
69	10982.0
70	9523.0
71	7456.5
72	5390.0
73	4510.0
74	3630.0
75	2783.0
76	1936.0
77	1696.5
78	1457.0
79	1197.0
80	937.0
81	748.0
82	559.0
83	455.5
84	352.0
85	243.5
86	135.0
87	95.0
88	55.0
89	42.5
90	30.0
91	30.5
92	31.0
93	24.5
94	18.0
95	17.0
96	16.0
97	12.5
98	9.0
99	8.0
100	7.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.010061892912009974
2	6.135300556103642E-4
3	1.2270601112207284E-4
4	0.0
5	0.0
6	3.6811803336621855E-4
7	2.454120222441457E-4
8	0.0
9	0.0013497661223428013
10	7.362360667324371E-4
11	0.0
12	0.0
13	3.6811803336621855E-4
14	1.2270601112207284E-4
15	0.004785534433760841
16	7.362360667324371E-4
17	0.0
18	2.454120222441457E-4
19	2.454120222441457E-4
20	8.5894207785451E-4
21	0.0
22	0.0
23	9.816480889765827E-4
24	9.816480889765827E-4
25	0.0014724721334648742
26	0.0036811803336621856
27	0.00601259454498157
28	0.0036811803336621856
29	0.003190356289173894
30	0.0036811803336621856
31	0.0066261246005919336
32	0.002454120222441457
33	0.004417416400394622
34	0.0026995322446856027
35	0.0026995322446856027
36	0.008098596734056808
37	0.004049298367028404
38	0.007607772689568516
39	0.003803886344784258
40	0.00257682623356353
41	0.002454120222441457
42	0.001595178144586947
43	0.0013497661223428013
44	7.362360667324371E-4
45	0.0012270601112207285
46	7.362360667324371E-4
47	0.002454120222441457
48	0.0018405901668310928
49	1.2270601112207284E-4
50	0.0013497661223428013
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	814956.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	61.91654065296126
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.34875521881607	48.51083887614735
2	13.541896579399342	16.769347801531527
3	3.909412637827809	7.261719195577982
4	1.6419404770177823	4.066530971800565
5	0.7690100511628303	2.38072210476796
6	0.45085322302712943	1.674916314724672
7	0.28505740001869084	1.2354837667679297
8	0.1950507702993168	0.9661495158903246
9	0.13439349475142046	0.7489062253142874
>10	0.6132166270355573	6.833547350165686
>50	0.05710463599129843	2.535806970014946
>100	0.050912979734998205	6.101600549816602
>500	0.0023959049178135373	0.9144303574800509
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	2.454120222441457E-4
2	0.0	0.0	0.0	0.0	2.454120222441457E-4
3	0.0	0.0	0.0	0.0	2.454120222441457E-4
4	0.0	0.0	0.0	0.0	2.454120222441457E-4
5	0.0	0.0	0.0	0.0	2.454120222441457E-4
6	0.0	0.0	0.0	0.0	2.454120222441457E-4
7	0.0	0.0	0.0	0.0	2.454120222441457E-4
8	0.0	0.0	0.0	0.0	2.454120222441457E-4
9	0.0	0.0	0.0	0.0	2.454120222441457E-4
10	0.0	0.0	0.0	0.0	2.454120222441457E-4
11	0.0	0.0	0.0	0.0	2.454120222441457E-4
12	0.0	0.0	0.0	1.2270601112207284E-4	3.6811803336621855E-4
13	0.0	0.0	0.0	1.2270601112207284E-4	4.908240444882914E-4
14	0.0	0.0	0.0	1.2270601112207284E-4	4.908240444882914E-4
15	0.0	0.0	0.0	1.2270601112207284E-4	6.135300556103642E-4
16	0.0	0.0	0.0	1.2270601112207284E-4	6.135300556103642E-4
17	0.0	0.0	0.0	1.2270601112207284E-4	6.135300556103642E-4
18	0.0	0.0	0.0	1.2270601112207284E-4	7.362360667324371E-4
19	0.0	1.2270601112207284E-4	0.0	1.2270601112207284E-4	7.362360667324371E-4
20	0.0	1.2270601112207284E-4	0.0	1.2270601112207284E-4	7.362360667324371E-4
21	0.0	1.2270601112207284E-4	0.0	2.454120222441457E-4	7.362360667324371E-4
22	0.0	1.2270601112207284E-4	0.0	2.454120222441457E-4	7.362360667324371E-4
23	0.0	1.2270601112207284E-4	0.0	0.0014724721334648742	8.5894207785451E-4
24	0.0	1.2270601112207284E-4	0.0	0.00257682623356353	9.816480889765827E-4
25	0.0	1.2270601112207284E-4	0.0	0.003067650278051821	9.816480889765827E-4
26	0.0	1.2270601112207284E-4	0.0	0.0038038863447842582	0.0011043541000986556
27	0.0	2.454120222441457E-4	0.0	0.0045401224115166955	0.0011043541000986556
28	0.0	2.454120222441457E-4	0.0	0.006258006567225715	0.0011043541000986556
29	0.0	2.454120222441457E-4	0.0	0.009816480889765828	0.0011043541000986556
30	0.0	2.454120222441457E-4	0.0	0.017178841557090197	0.0011043541000986556
31	0.0	2.454120222441457E-4	0.0	0.03153544485837272	0.0011043541000986556
32	0.0	2.454120222441457E-4	0.0	0.0496959345044395	0.0011043541000986556
33	0.0	2.454120222441457E-4	0.0	0.06270277168337922	0.0011043541000986556
34	0.0	2.454120222441457E-4	0.0	0.08135408537393429	0.0011043541000986556
35	0.0	2.454120222441457E-4	0.0	0.10196869524244254	0.0011043541000986556
36	0.0	2.454120222441457E-4	0.0	0.13031378381164135	0.0011043541000986556
37	0.0	3.6811803336621855E-4	0.0	0.17326088770436687	0.0011043541000986556
38	0.0	3.6811803336621855E-4	0.0	0.2336322451764267	0.0011043541000986556
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTAATAC	110	7.2759576E-12	25.996048	3
TGAACGG	65	4.9332797E-4	20.305851	10
CGCCCCT	120	1.4322723E-8	20.1636	5
TAATACT	145	2.9649527E-10	19.72114	4
CTATACT	125	2.2891982E-8	19.357058	4
TAATACC	70	8.1285264E-4	18.854275	4
GTGTAGA	200	0.0	18.697157	1
GTATCAA	1725	0.0	18.61746	1
CTAGCGG	180	1.4551915E-11	18.335047	29
TACGAAT	180	1.4551915E-11	18.333921	41
GTGCTAT	120	3.1638774E-7	18.330547	1
CGCCGGT	180	1.4551915E-11	18.330547	7
TAAGACC	120	3.1638774E-7	18.330547	4
CGTCGTA	195	1.8189894E-12	18.049646	10
AGACCGT	75	0.0012916966	17.597324	6
TAGGACG	150	9.600626E-9	17.597324	4
TCTATAC	100	2.4846473E-5	17.597324	3
CGCAATA	190	3.274181E-11	17.371109	36
TCTAGCG	190	3.274181E-11	17.370045	28
GACGTGA	115	4.2756237E-6	17.214773	7
>>END_MODULE