FastQCFastQC Report
Thu 2 Feb 2017
SRR4063997_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4063997_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1018119
Sequences flagged as poor quality0
Sequence length25
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT41260.4052571457756902No Hit
TATCAACGCAGAGTACTTTTTTTTT30660.3011435794833413No Hit
GTACTTTTTTTTTTTTTTTTTTTTT24140.23710391417899085No Hit
GTACATGGGGTGGTATCAACGCAAA19660.19310119936863962No Hit
ACGCAGAGTACTTTTTTTTTTTTTT15590.15312551872619998No Hit
GGTATCAACGCAGAGTACTTTTTTT14750.14487500969925912No Hit
GAGTACTTTTTTTTTTTTTTTTTTT14570.14310704347920036No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAACGA359.925876E-519.0023616
CGCGTAA551.957041E-413.81989810
TCGCGTA551.9578575E-413.8192199
CGTCGTA903.6279744E-813.723927510
CGAGCCG1205.0931703E-1113.46000415
ACGGTAT852.6934504E-713.4127719
GAGCGTT500.001498633913.3003447
ATAATTC500.001502525213.2957693
CGCATCG1301.4551915E-1113.1554813
ATCCCCG1102.732122E-912.95742812
CCGTCGT1051.9881554E-812.6676179
GGGTCGC751.4784942E-512.665756
GTCTTAG905.4132215E-712.6638811
TAAGGCT1155.331458E-912.3885794
ATACCGT1551.8189894E-1212.2571766
TAACGCC1551.8189894E-1212.2553684
CCCGATC1251.4042598E-912.1615115
AGCGTTC550.003065469212.0918178
GATATAA550.003072249212.0882491
ATCGCCA1501.4551915E-1112.03482816
Produced by FastQC (version 0.11.5)