##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4063997_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1018119
Sequences flagged as poor quality	0
Sequence length	25
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.198208657337698	32.0	32.0	32.0	32.0	32.0
2	30.809454494022802	32.0	32.0	32.0	32.0	32.0
3	30.84138396395706	32.0	32.0	32.0	32.0	32.0
4	30.861302067832934	32.0	32.0	32.0	32.0	32.0
5	30.782244511692642	32.0	32.0	32.0	32.0	32.0
6	34.42493264539803	36.0	36.0	36.0	32.0	36.0
7	34.33962336426292	36.0	36.0	36.0	32.0	36.0
8	34.292967717919026	36.0	36.0	36.0	32.0	36.0
9	34.422697150333114	36.0	36.0	36.0	32.0	36.0
10	34.13324179197127	36.0	36.0	36.0	32.0	36.0
11	34.43993089216487	36.0	36.0	36.0	32.0	36.0
12	34.23932762280244	36.0	36.0	36.0	32.0	36.0
13	34.31821525774492	36.0	36.0	36.0	32.0	36.0
14	34.22577223291187	36.0	36.0	36.0	32.0	36.0
15	34.171946501342184	36.0	36.0	36.0	32.0	36.0
16	34.16228653035647	36.0	36.0	36.0	32.0	36.0
17	34.08961918989824	36.0	36.0	36.0	32.0	36.0
18	34.08014976638291	36.0	36.0	36.0	32.0	36.0
19	34.09920451342132	36.0	36.0	36.0	32.0	36.0
20	34.06772194605935	36.0	36.0	36.0	32.0	36.0
21	34.04938420754352	36.0	36.0	36.0	32.0	36.0
22	34.021510255677384	36.0	36.0	36.0	32.0	36.0
23	33.97857126720943	36.0	36.0	36.0	32.0	36.0
24	33.97659409165333	36.0	36.0	36.0	32.0	36.0
25	33.57537380208011	36.0	36.0	36.0	27.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
3	2.0
4	4.0
5	18.0
6	45.0
7	14.0
8	60.0
9	80.0
10	78.0
11	37.0
12	76.0
13	39.0
14	120.0
15	181.0
16	346.0
17	439.0
18	592.0
19	807.0
20	1191.0
21	1604.0
22	2776.0
23	3965.0
24	5966.0
25	8513.0
26	11961.0
27	15757.0
28	21198.0
29	28243.0
30	37061.0
31	49490.0
32	69430.0
33	96022.0
34	204227.0
35	457777.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.835544183596866	17.424644397017612	11.756220841341525	24.983590578043994
2	15.940266605897314	20.1546671748242	38.78142382990343	25.123642389375057
3	18.6042696971334	25.224903128852134	28.86460630288624	27.30622087112822
4	11.9302650176852	15.972469828098495	36.97321338240595	35.12405177181036
5	13.787128372471322	37.748300134967614	33.910563818856566	14.554007673704497
6	32.514816120961235	36.62099119922906	17.959506040890822	12.904686638918877
7	29.34902104105939	31.159866662343898	21.26633440321771	18.224777893378995
8	28.011327236067935	33.181392990634	19.754766518230877	19.05251325506719
9	27.794925919047014	14.095968663614396	18.763112923212688	39.3459924941259
10	15.419119157388234	27.582817937637532	32.4614276321544	24.536635272819833
11	36.30466145669539	21.705276275237438	22.96026872759293	19.029793540474245
12	24.37767329223802	24.3259765379293	29.90637580198767	21.389974367845014
13	29.52511347307214	20.303374607586626	25.77288480099703	24.39862711834421
14	23.33870634834829	20.12806777861868	26.498825472022645	30.03440040101039
15	24.719384922019426	27.85855879673355	23.377267942804743	24.044788338442274
16	24.655161907307992	26.515233420546195	24.84141477481586	23.98818989732995
17	23.134017376262925	26.441011125525808	26.077269332075325	24.347702166135942
18	23.61409140995686	25.931827363615902	27.37629616377789	23.07778506264935
19	24.799893053489967	25.63501947743655	26.42148917117438	23.14359829789911
20	25.20514766306634	24.90583063669499	26.243959595641865	23.6450621045968
21	26.394527064882983	24.712543125903064	25.403245623519467	23.489684185694486
22	25.14786355955778	25.004153056600508	25.919988047060766	23.927995336780945
23	24.09540015315456	24.956132719535464	26.177633732537874	24.7708333947721
24	23.913917446225437	25.833121180637125	26.197499579790218	24.05546179334722
25	24.177568687894357	25.349865676351275	26.19915680332324	24.273408832431127
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	152.0
1	152.0
2	435.0
3	718.0
4	718.0
5	718.0
6	1903.5
7	3089.0
8	3089.0
9	3089.0
10	2948.5
11	2808.0
12	2808.0
13	2808.0
14	2964.0
15	3120.0
16	3120.0
17	3120.0
18	5566.0
19	8012.0
20	8012.0
21	8012.0
22	12786.0
23	17560.0
24	17560.0
25	17560.0
26	25996.5
27	34433.0
28	34433.0
29	34433.0
30	43731.0
31	53029.0
32	53029.0
33	53029.0
34	61924.5
35	70820.0
36	70820.0
37	70820.0
38	80821.5
39	90823.0
40	90823.0
41	90823.0
42	102104.5
43	113386.0
44	113386.0
45	113386.0
46	126169.0
47	138952.0
48	138952.0
49	138952.0
50	138803.5
51	138655.0
52	138655.0
53	138655.0
54	125421.5
55	112188.0
56	112188.0
57	112188.0
58	100437.0
59	88686.0
60	88686.0
61	88686.0
62	75788.0
63	62890.0
64	62890.0
65	62890.0
66	51139.0
67	39388.0
68	39388.0
69	39388.0
70	29311.5
71	19235.0
72	19235.0
73	19235.0
74	14519.5
75	9804.0
76	9804.0
77	9804.0
78	7804.5
79	5805.0
80	5805.0
81	5805.0
82	3906.0
83	2007.0
84	2007.0
85	2007.0
86	1549.5
87	1092.0
88	1092.0
89	1092.0
90	800.0
91	508.0
92	508.0
93	508.0
94	377.0
95	246.0
96	246.0
97	246.0
98	479.5
99	713.0
100	713.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.04036856202467492
2	0.006089661424646824
3	0.0013750848378234766
4	0.003535932440117511
5	0.009527373519205516
6	0.013750848378234764
7	0.024751527080822573
8	0.0387970364957338
9	0.05068169830835099
10	0.06050373286423296
11	0.05726246146079191
12	0.06354856357655637
13	0.06845958085449735
14	0.0676738180900268
15	0.06109305493758588
16	0.06728093670779153
17	0.06276280081208581
18	0.0775940729914676
19	0.07739763230034996
20	0.07887093748373226
21	0.07356703882355599
22	0.07818339506482051
23	0.0830944123427615
24	0.0756296660802912
25	0.0785762764470558
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	1018119.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	54.281490057240546
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.71907236082492	40.01581093383114
2	15.33267641337831	16.64561044567363
3	5.213321174506766	8.489605244975719
4	2.253478317664755	4.892886435781062
5	1.101287213458173	2.9889755463747987
6	0.6127817537811003	1.9957624005076335
7	0.38956454908645644	1.4802300938522919
8	0.24144958161619176	1.0484994451059362
9	0.16347928232592107	0.7986509134325375
>10	0.8183270650901113	8.123508136848193
>50	0.08001679090030869	3.0613986310026218
>100	0.0680000395262531	7.009125914921434
>500	0.0050909116539598264	1.764149315032124
>1k	0.0014545461868456645	1.6857865426609067
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	4126	0.4052571457756902	No Hit
TATCAACGCAGAGTACTTTTTTTTT	3066	0.3011435794833413	No Hit
GTACTTTTTTTTTTTTTTTTTTTTT	2414	0.23710391417899085	No Hit
GTACATGGGGTGGTATCAACGCAAA	1966	0.19310119936863962	No Hit
ACGCAGAGTACTTTTTTTTTTTTTT	1559	0.15312551872619998	No Hit
GGTATCAACGCAGAGTACTTTTTTT	1475	0.14487500969925912	No Hit
GAGTACTTTTTTTTTTTTTTTTTTT	1457	0.14310704347920036	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.964406911176395E-4
2	0.0	0.0	0.0	0.0	1.964406911176395E-4
3	0.0	0.0	0.0	0.0	1.964406911176395E-4
4	0.0	0.0	0.0	0.0	1.964406911176395E-4
5	0.0	0.0	0.0	0.0	1.964406911176395E-4
6	0.0	0.0	0.0	0.0	1.964406911176395E-4
7	0.0	0.0	0.0	0.0	1.964406911176395E-4
8	0.0	0.0	0.0	0.0	1.964406911176395E-4
9	0.0	0.0	0.0	0.0	1.964406911176395E-4
10	0.0	0.0	0.0	0.0	1.964406911176395E-4
11	0.0	0.0	0.0	9.822034555881975E-5	1.964406911176395E-4
12	0.0	0.0	0.0	9.822034555881975E-5	1.964406911176395E-4
13	0.0	0.0	0.0	9.822034555881975E-5	4.911017277940987E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGAACGA	35	9.925876E-5	19.00236	16
CGCGTAA	55	1.957041E-4	13.819898	10
TCGCGTA	55	1.9578575E-4	13.819219	9
CGTCGTA	90	3.6279744E-8	13.7239275	10
CGAGCCG	120	5.0931703E-11	13.460004	15
ACGGTAT	85	2.6934504E-7	13.412771	9
GAGCGTT	50	0.0014986339	13.300344	7
ATAATTC	50	0.0015025252	13.295769	3
CGCATCG	130	1.4551915E-11	13.15548	13
ATCCCCG	110	2.732122E-9	12.957428	12
CCGTCGT	105	1.9881554E-8	12.667617	9
GGGTCGC	75	1.4784942E-5	12.66575	6
GTCTTAG	90	5.4132215E-7	12.663881	1
TAAGGCT	115	5.331458E-9	12.388579	4
ATACCGT	155	1.8189894E-12	12.257176	6
TAACGCC	155	1.8189894E-12	12.255368	4
CCCGATC	125	1.4042598E-9	12.16151	15
AGCGTTC	55	0.0030654692	12.091817	8
GATATAA	55	0.0030722492	12.088249	1
ATCGCCA	150	1.4551915E-11	12.034828	16
>>END_MODULE