FastQCFastQC Report
Thu 2 Feb 2017
SRR4063997_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4063997_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1018119
Sequences flagged as poor quality0
Sequence length50
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11850.1163911094872014No Hit
GTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA11660.11452492292158382No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACGAAT1301.8189894E-1223.69545431
CGTAAAC904.0829946E-721.998640
CCGATTG808.987799E-519.249729
CTCGCGG808.987799E-519.2497214
ATCCCGT1557.203198E-1018.45134520
CCAACGA1456.3009793E-918.20752729
CGATTGC851.430711E-418.11738410
CAACGAA1951.8189894E-1218.0536830
GTCGTAA1102.7946207E-618.00062438
CCCGTTT1601.1004886E-917.8747422
CGTGATT1002.481651E-517.60060928
TCGCGGC750.001290806617.59974515
ACGAACG1002.4826988E-517.59974515
GTATAGA1254.851354E-717.5997431
AATCCCG1509.598807E-917.5988819
GTACCGA902.216016E-417.1100226
GTGTATA3650.016.8764671
TTAAGGG1053.7754584E-516.760843
GTATCAA32750.016.5921251
GTATTAG2400.016.499761
Produced by FastQC (version 0.11.5)