##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4063997_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1018119
Sequences flagged as poor quality	0
Sequence length	50
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.239861941482282	32.0	32.0	32.0	32.0	32.0
2	31.396408474844296	32.0	32.0	32.0	32.0	32.0
3	31.485421645210433	32.0	32.0	32.0	32.0	32.0
4	31.56816737532646	32.0	32.0	32.0	32.0	32.0
5	31.51884995761792	32.0	32.0	32.0	32.0	32.0
6	35.095762872512935	36.0	36.0	36.0	36.0	36.0
7	35.11889278168859	36.0	36.0	36.0	36.0	36.0
8	35.074094482079204	36.0	36.0	36.0	36.0	36.0
9	35.179221682337726	36.0	36.0	36.0	36.0	36.0
10	35.0364446592196	36.0	36.0	36.0	36.0	36.0
11	35.18426529708217	36.0	36.0	36.0	36.0	36.0
12	35.103047875543034	36.0	36.0	36.0	36.0	36.0
13	35.12752045684247	36.0	36.0	36.0	36.0	36.0
14	35.08063595709343	36.0	36.0	36.0	36.0	36.0
15	35.054174413796424	36.0	36.0	36.0	36.0	36.0
16	35.06778873589433	36.0	36.0	36.0	36.0	36.0
17	35.034341761621185	36.0	36.0	36.0	36.0	36.0
18	35.03321517425763	36.0	36.0	36.0	36.0	36.0
19	35.026955591635165	36.0	36.0	36.0	36.0	36.0
20	35.01412703230172	36.0	36.0	36.0	36.0	36.0
21	35.006036622438046	36.0	36.0	36.0	36.0	36.0
22	34.99332396311237	36.0	36.0	36.0	36.0	36.0
23	34.942643247007474	36.0	36.0	36.0	36.0	36.0
24	34.922828274494435	36.0	36.0	36.0	36.0	36.0
25	34.898252561832166	36.0	36.0	36.0	32.0	36.0
26	34.8408545562945	36.0	36.0	36.0	32.0	36.0
27	34.83115726157748	36.0	36.0	36.0	32.0	36.0
28	34.7996992493019	36.0	36.0	36.0	32.0	36.0
29	34.778875553839974	36.0	36.0	36.0	32.0	36.0
30	34.762606335801614	36.0	36.0	36.0	32.0	36.0
31	34.74086526231216	36.0	36.0	36.0	32.0	36.0
32	34.7057888125062	36.0	36.0	36.0	32.0	36.0
33	34.66684640989904	36.0	36.0	36.0	32.0	36.0
34	34.653354863233076	36.0	36.0	36.0	32.0	36.0
35	34.599230541812894	36.0	36.0	36.0	32.0	36.0
36	34.569310660148766	36.0	36.0	36.0	32.0	36.0
37	34.54900458590794	36.0	36.0	36.0	32.0	36.0
38	34.510620074863546	36.0	36.0	36.0	32.0	36.0
39	34.490800191333236	36.0	36.0	36.0	32.0	36.0
40	34.459946234182844	36.0	36.0	36.0	32.0	36.0
41	34.43542552491408	36.0	36.0	36.0	32.0	36.0
42	34.36149114199814	36.0	36.0	36.0	32.0	36.0
43	34.32808640247358	36.0	36.0	36.0	32.0	36.0
44	34.26320204219743	36.0	36.0	36.0	32.0	36.0
45	34.21217657267962	36.0	36.0	36.0	32.0	36.0
46	34.17687912709614	36.0	36.0	36.0	32.0	36.0
47	34.12847614080476	36.0	36.0	36.0	32.0	36.0
48	34.06324408050532	36.0	36.0	36.0	32.0	36.0
49	34.02757045099836	36.0	36.0	36.0	32.0	36.0
50	33.47088798067809	36.0	36.0	36.0	21.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	1.0
18	1.0
19	2.0
20	15.0
21	35.0
22	123.0
23	306.0
24	873.0
25	1955.0
26	4098.0
27	7476.0
28	12694.0
29	18723.0
30	26673.0
31	37070.0
32	53045.0
33	83252.0
34	170769.0
35	601008.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.80079723541234	18.48356315900112	12.310882518776681	25.40475708680986
2	15.200821910273485	21.008313394544434	38.26636650440618	25.5244981907759
3	17.77255025218173	25.7434572715263	29.27390324275745	27.210089233534525
4	11.582830690714935	16.666715776839446	36.9790761197856	34.771377412660016
5	13.321330807106047	38.231581966351676	33.77355692212796	14.673530304414317
6	32.542590964674915	37.140598066033796	17.386149894658264	12.930661074633024
7	29.122627119226728	31.35645243827097	21.29181362885871	18.229106813643593
8	27.798223979711604	33.53429216034668	19.677071147871715	18.990412712070004
9	27.63919242121392	13.991484178940286	18.679703959807682	39.68961944003811
10	15.213701574105542	27.731798974955357	32.551453587165135	24.503045863773963
11	36.40923743613216	21.592880196598035	22.95500128668779	19.042881080582013
12	24.153487120353436	24.367411243097557	30.040722195266166	21.438379441282837
13	29.5225579086015	20.28841592239761	25.87551676483848	24.313509404162406
14	23.255035040245943	20.01355446094007	26.615166263143163	30.116244235670823
15	24.766472641692907	27.867205195652218	23.473354798532927	23.892967364121947
16	24.663937437261445	26.468254046207008	24.92441907291325	23.943389443618297
17	23.155446465491757	26.35654574759925	26.17041819276529	24.31758959414371
18	23.625153961147767	25.75693881038862	27.53119984598974	23.086707382473868
19	24.845818612559043	25.53954891324099	26.611918646052178	23.00271382814779
20	25.135176281657767	24.753588739975346	26.515766882915976	23.595468095450904
21	26.278657013571106	24.582686306806963	25.707702144837686	23.43095453478424
22	24.99658684299183	24.913099549266835	26.24978023197681	23.840533375764522
23	24.004427821017025	24.78420756933449	26.51457409233598	24.696790517312508
24	23.925634973720992	25.62377653561049	26.42977744120741	24.02081104946111
25	24.297042209389925	25.1064001155103	26.46551241490201	24.131045260197762
26	23.740620650432536	25.746296145319388	26.933847775329767	23.579235428918306
27	24.415035366528066	25.598274353103424	26.078897660534373	23.907792619834133
28	23.714114803162445	25.368514831451183	26.759770664775512	24.15759970061086
29	23.87676924436936	25.45295602549873	26.539402213949653	24.130872516182265
30	23.79761059330483	25.693243241915887	26.82989597054054	23.679250194238747
31	24.136992787113265	25.570336696334817	26.124539433165133	24.168131083386786
32	23.771263105577372	25.689780154369313	26.17952651957905	24.359430220474266
33	23.703136909766695	25.18731484610126	26.47956136697601	24.629986877156036
34	24.121222955511833	25.555082993563445	26.54291217582711	23.78078187509761
35	24.814824637138795	25.237086345103428	26.377949275554496	23.570139742203285
36	23.81960379078393	26.158982680580877	26.008596844156664	24.01281668447853
37	24.48348954209975	26.0725560416548	25.801850931457388	23.642103484788063
38	23.89676955462151	25.91035850507793	26.200034772939578	23.99283716736098
39	24.29012321425765	25.659290575052257	26.04286174543054	24.007724465259553
40	24.614499096839086	25.966244667716342	26.032741670440068	23.386514565004504
41	23.782764913769565	26.1320752863707	26.426058309415907	23.65910149044383
42	24.631370715531737	26.426879194103503	25.832730901604563	23.109019188760197
43	23.975546702497976	25.643942072715557	26.091342338307282	24.28916888647918
44	23.716708983847443	26.054188909788284	25.790856542301626	24.438245564062647
45	23.99725371867707	26.29642944139302	25.68215035006247	24.02416648986744
46	23.80488868546104	26.120757563554285	25.53083863566269	24.54351511532198
47	24.121222955511833	26.03951906067336	25.84896634976638	23.99029163404843
48	24.473575131569383	26.4005091867745	25.112710614147616	24.0132050675085
49	23.878049139831944	26.475692824484465	25.588072074372736	24.058185961310855
50	23.941318487465942	26.701934769071194	25.221047710159844	24.135699033303016
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	26.0
1	26.5
2	27.0
3	79.0
4	131.0
5	164.0
6	197.0
7	218.0
8	239.0
9	355.5
10	472.0
11	695.0
12	918.0
13	1703.5
14	2489.0
15	3441.0
16	4393.0
17	4890.5
18	5388.0
19	5401.0
20	5414.0
21	5748.5
22	6083.0
23	6829.5
24	7576.0
25	7700.0
26	7824.0
27	9471.5
28	11119.0
29	13337.0
30	15555.0
31	17740.5
32	19926.0
33	23420.5
34	26915.0
35	30652.5
36	34390.0
37	39186.5
38	43983.0
39	46939.0
40	49895.0
41	53521.0
42	57147.0
43	58640.5
44	60134.0
45	65415.5
46	70697.0
47	73752.0
48	76807.0
49	78229.0
50	79651.0
51	77105.0
52	74559.0
53	71946.0
54	69333.0
55	67236.0
56	65139.0
57	62531.5
58	59924.0
59	53545.0
60	47166.0
61	41223.5
62	35281.0
63	30095.5
64	24910.0
65	21084.5
66	17259.0
67	14796.0
68	12333.0
69	10877.5
70	9422.0
71	7410.5
72	5399.0
73	4565.0
74	3731.0
75	2924.5
76	2118.0
77	1867.0
78	1616.0
79	1328.0
80	1040.0
81	837.0
82	634.0
83	528.5
84	423.0
85	306.5
86	190.0
87	150.5
88	111.0
89	76.0
90	41.0
91	37.5
92	34.0
93	29.5
94	25.0
95	22.5
96	20.0
97	15.5
98	11.0
99	7.5
100	4.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.009920254901440793
2	2.946610366764593E-4
3	3.92881382235279E-4
4	0.0
5	0.0
6	3.92881382235279E-4
7	0.0
8	0.0
9	0.0013750848378234766
10	0.0016697458744999358
11	9.822034555881975E-5
12	9.822034555881975E-5
13	5.893220733529186E-4
14	3.92881382235279E-4
15	0.0036341527856763307
16	4.911017277940987E-4
17	0.0
18	4.911017277940987E-4
19	0.0
20	3.92881382235279E-4
21	0.0
22	0.0
23	0.0010804238011470173
24	5.893220733529186E-4
25	0.002750169675646953
26	0.005696780042411546
27	0.006482542806882104
28	0.004518135895705709
29	0.002848390021205773
30	0.0041252545134704294
31	0.00785762764470558
32	0.00402703416791161
33	0.004616356241264529
34	0.002750169675646953
35	0.0029466103667645923
36	0.006973644534676202
37	0.0036341527856763307
38	0.008446949718058499
39	0.005402119005735086
40	0.002750169675646953
41	0.003241271403441052
42	0.0022590679478528544
43	0.0014733051833822962
44	0.0013750848378234766
45	0.0014733051833822962
46	0.0012768644922646567
47	0.002750169675646953
48	0.0024555086389704936
49	3.92881382235279E-4
50	0.0012768644922646567
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	1018119.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	57.97176595108393
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.78583574917346	43.35466966507289
2	14.96324119578141	17.348910329429142
3	5.0189415580559364	8.72870715977362
4	2.09603786003509	4.860440649862603
5	1.0005744843642181	2.900253491209447
6	0.5745234806562081	1.9983684452402277
7	0.34183327661775464	1.3871675094463625
8	0.22491401734392968	1.043093021806426
9	0.158350998585531	0.8261898325308744
>10	0.7134039380389496	7.5168215191983165
>50	0.06441841694775768	2.672435150059591
>100	0.05400656748156879	5.838107925488023
>500	0.00357772153400176	1.2926384103860866
>1k	3.4073538419064376E-4	0.2321968904964616
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1185	0.1163911094872014	No Hit
GTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1166	0.11452492292158382	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	9.822034555881975E-5
8	0.0	0.0	0.0	0.0	9.822034555881975E-5
9	0.0	0.0	0.0	9.822034555881975E-5	9.822034555881975E-5
10	0.0	0.0	0.0	9.822034555881975E-5	9.822034555881975E-5
11	0.0	0.0	0.0	9.822034555881975E-5	9.822034555881975E-5
12	0.0	0.0	0.0	9.822034555881975E-5	2.946610366764592E-4
13	0.0	0.0	0.0	9.822034555881975E-5	4.911017277940987E-4
14	0.0	0.0	0.0	2.946610366764592E-4	4.911017277940987E-4
15	0.0	0.0	0.0	2.946610366764592E-4	6.875424189117382E-4
16	0.0	0.0	0.0	3.92881382235279E-4	9.822034555881974E-4
17	0.0	0.0	0.0	5.893220733529184E-4	0.0011786441467058369
18	0.0	0.0	0.0	7.85762764470558E-4	0.0011786441467058369
19	0.0	0.0	0.0	8.839831100293777E-4	0.0011786441467058369
20	0.0	0.0	0.0	9.822034555881974E-4	0.0012768644922646567
21	0.0	0.0	0.0	0.0010804238011470173	0.0012768644922646567
22	0.0	0.0	0.0	0.001964406911176395	0.0012768644922646567
23	0.0	0.0	0.0	0.0032412714034410514	0.0012768644922646567
24	0.0	0.0	0.0	0.004419915550146889	0.0012768644922646567
25	0.0	0.0	0.0	0.005107457969058627	0.0012768644922646567
26	0.0	0.0	0.0	0.005893220733529185	0.0012768644922646567
27	0.0	0.0	0.0	0.0074647462624703	0.0014733051833822962
28	0.0	0.0	0.0	0.008839831100293778	0.0014733051833822962
29	0.0	0.0	0.0	0.014143729760470044	0.0014733051833822962
30	0.0	0.0	0.0	0.02298356086076382	0.0014733051833822962
31	0.0	0.0	0.0	0.04056500271579255	0.0014733051833822962
32	0.0	0.0	0.0	0.0611912752831447	0.0016697458744999356
33	0.0	0.0	0.0	0.07847805610149698	0.0016697458744999356
34	0.0	0.0	0.0	0.097532803139908	0.0017679662200587554
35	0.0	0.0	0.0	0.12346297436743642	0.0017679662200587554
36	0.0	0.0	0.0	0.15705433254855278	0.0017679662200587554
37	0.0	0.0	0.0	0.2053787425634921	0.0017679662200587554
38	0.0	0.0	0.0	0.2730525606535189	0.0017679662200587554
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AACGAAT	130	1.8189894E-12	23.695454	31
CGTAAAC	90	4.0829946E-7	21.9986	40
CCGATTG	80	8.987799E-5	19.24972	9
CTCGCGG	80	8.987799E-5	19.24972	14
ATCCCGT	155	7.203198E-10	18.451345	20
CCAACGA	145	6.3009793E-9	18.207527	29
CGATTGC	85	1.430711E-4	18.117384	10
CAACGAA	195	1.8189894E-12	18.05368	30
GTCGTAA	110	2.7946207E-6	18.000624	38
CCCGTTT	160	1.1004886E-9	17.87474	22
CGTGATT	100	2.481651E-5	17.600609	28
TCGCGGC	75	0.0012908066	17.599745	15
ACGAACG	100	2.4826988E-5	17.599745	15
GTATAGA	125	4.851354E-7	17.599743	1
AATCCCG	150	9.598807E-9	17.59888	19
GTACCGA	90	2.216016E-4	17.110022	6
GTGTATA	365	0.0	16.876467	1
TTAAGGG	105	3.7754584E-5	16.76084	3
GTATCAA	3275	0.0	16.592125	1
GTATTAG	240	0.0	16.49976	1
>>END_MODULE