FastQCFastQC Report
Thu 2 Feb 2017
SRR4063996_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4063996_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1067938
Sequences flagged as poor quality0
Sequence length25
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTAAAGGTATAAGGGAGCTTGAC25360.23746696905625608No Hit
GTATCAACGCAGAGTACTTTTTTTT23340.21855201331912524No Hit
CCCTTATACCTTTACACTCTGCGAA22970.21508739271380922No Hit
CCTTTACACTCTGCGAATGATTTCC21030.1969215441345846No Hit
GTATAAGGGAGCTTGACTGCGAGAG16710.1564697576076514No Hit
GTCCTAAGGTAGCGAAATTCCTTGT15480.144952235054844No Hit
TATCAACGCAGAGTACTTTTTTTTT15450.1446713198706292No Hit
GTCTCAACGAGAGACTCGGTGAAAT12740.11929531489655767No Hit
GTCCCAAGGGTTGGGCTGTTCGCCC10980.10281495742262192No Hit
GTACTTTTTTTTTTTTTTTTTTTTT10860.10169129668576266No Hit
GGTATAAGGGAGCTTGACTGCGAGA10820.10131674310680956No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCTTAT2950.014.8081161
CGAATGA2850.014.66814914
CTTATAC2850.014.6633393
TTATACC3050.014.6373964
CGTCGTA1300.014.61686210
AGGTATA3650.014.5768177
GTGTAAA4450.014.511521
GCGAAAT2500.014.440783512
TGTAAAG3750.014.4340192
CCTTATA3100.014.3972612
CGAAATT2450.014.34771713
CCGTCGT1400.014.2521099
TTACAAT954.8876245E-914.00141516
TAGGCCC1900.013.99813654
GTAAAGG3800.013.9968233
TTACGCT759.648411E-713.93539615
ACCGTTA1850.013.8669178
TTGGGGC551.961461E-413.8163414
GGACCGT2000.013.7744576
GACCGTT1900.013.5013657
Produced by FastQC (version 0.11.5)