##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4063996_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1067938
Sequences flagged as poor quality	0
Sequence length	25
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.25693907324208	32.0	32.0	32.0	32.0	32.0
2	30.863040738320016	32.0	32.0	32.0	32.0	32.0
3	30.8673209493435	32.0	32.0	32.0	32.0	32.0
4	30.90565650814935	32.0	32.0	32.0	32.0	32.0
5	30.808620912449975	32.0	32.0	32.0	32.0	32.0
6	34.461748715749415	36.0	36.0	36.0	32.0	36.0
7	34.41247525605419	36.0	36.0	36.0	32.0	36.0
8	34.362028507272896	36.0	36.0	36.0	32.0	36.0
9	34.49550629343651	36.0	36.0	36.0	32.0	36.0
10	34.212410271008245	36.0	36.0	36.0	32.0	36.0
11	34.477958458262556	36.0	36.0	36.0	32.0	36.0
12	34.29964848146615	36.0	36.0	36.0	32.0	36.0
13	34.38062415608397	36.0	36.0	36.0	32.0	36.0
14	34.284956617331716	36.0	36.0	36.0	32.0	36.0
15	34.212736132621934	36.0	36.0	36.0	32.0	36.0
16	34.21063395066006	36.0	36.0	36.0	32.0	36.0
17	34.135877738220756	36.0	36.0	36.0	32.0	36.0
18	34.14146514123479	36.0	36.0	36.0	32.0	36.0
19	34.149743711713604	36.0	36.0	36.0	32.0	36.0
20	34.13116959973332	36.0	36.0	36.0	32.0	36.0
21	34.111611348224336	36.0	36.0	36.0	32.0	36.0
22	34.087634300867656	36.0	36.0	36.0	32.0	36.0
23	34.04732765385256	36.0	36.0	36.0	32.0	36.0
24	34.01687082957999	36.0	36.0	36.0	32.0	36.0
25	33.644536480582204	36.0	36.0	36.0	27.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
3	4.0
4	15.0
5	14.0
6	39.0
7	19.0
8	65.0
9	58.0
10	100.0
11	32.0
12	57.0
13	49.0
14	87.0
15	156.0
16	299.0
17	376.0
18	482.0
19	653.0
20	1044.0
21	1489.0
22	2410.0
23	3680.0
24	5571.0
25	8045.0
26	11778.0
27	15698.0
28	21017.0
29	28566.0
30	38302.0
31	51782.0
32	73026.0
33	104800.0
34	224429.0
35	473796.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.361841643052855	17.05299641694574	10.998805648579658	26.586356291421747
2	16.977264448392013	19.043868873766606	38.09466731966959	25.884199358171788
3	19.273425999797738	23.298929878379948	28.822304375999607	28.605339745822704
4	12.486456976494836	15.515037555260685	36.80602831931664	35.19247714892784
5	14.585744734249573	36.32290858964418	33.694809354175334	15.39653732193092
6	33.308422137998654	36.667406510437885	16.766732878001637	13.257438473561823
7	29.831769853817164	30.74082329934139	20.156710099428306	19.27069674741314
8	28.36857708898287	33.175403839578856	18.934865792715197	19.521153278723073
9	28.553615492854828	13.602527182008442	17.989777353916026	39.8540799712207
10	16.88927989866226	26.002274852387565	31.02643813207261	26.08200711687757
11	37.81008808665883	20.73755946926898	22.637578019745952	18.814774424326234
12	24.660295151089247	23.71187631763879	29.31384399156711	22.313984539704848
13	29.792056488525297	19.551637322079603	25.024712792840738	25.631593396554358
14	23.585196484741385	20.468070233960155	24.196871723419346	31.74986155787911
15	25.822996395540475	27.178767887610572	22.08417776253667	24.91405795431228
16	25.881247253349628	25.85557280720084	23.700137461359493	24.563042478090047
17	23.887001944203696	25.792321566606546	25.023728654751586	25.29694783443817
18	24.491872870992076	24.994541305682198	26.68397205523355	23.829613768092177
19	26.905377027243567	24.007848936092454	24.4053559430671	24.68141809359688
20	25.411957642207856	24.389373067191453	24.971043013775652	25.22762627682504
21	27.972243566917182	22.59647281518826	23.91701182600221	25.514271791892345
22	25.611127458238297	24.650884712770562	24.699238890252882	25.03874893873825
23	24.746361532045697	24.766885021601208	25.600007497165134	24.886745949187965
24	25.318403442533693	25.448191910758013	24.236707927217253	24.99669671949104
25	24.709642837731717	24.784797410634464	25.074451755824477	25.431107995809338
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	95.0
1	95.0
2	216.0
3	337.0
4	337.0
5	337.0
6	900.0
7	1463.0
8	1463.0
9	1463.0
10	1458.0
11	1453.0
12	1453.0
13	1453.0
14	1559.5
15	1666.0
16	1666.0
17	1666.0
18	3125.0
19	4584.0
20	4584.0
21	4584.0
22	8046.0
23	11508.0
24	11508.0
25	11508.0
26	17799.0
27	24090.0
28	24090.0
29	24090.0
30	32425.0
31	40760.0
32	40760.0
33	40760.0
34	51373.0
35	61986.0
36	61986.0
37	61986.0
38	72712.5
39	83439.0
40	83439.0
41	83439.0
42	105416.5
43	127394.0
44	127394.0
45	127394.0
46	137152.0
47	146910.0
48	146910.0
49	146910.0
50	152169.0
51	157428.0
52	157428.0
53	157428.0
54	144073.5
55	130719.0
56	130719.0
57	130719.0
58	117638.0
59	104557.0
60	104557.0
61	104557.0
62	90644.0
63	76731.0
64	76731.0
65	76731.0
66	62209.5
67	47688.0
68	47688.0
69	47688.0
70	34826.5
71	21965.0
72	21965.0
73	21965.0
74	16370.0
75	10775.0
76	10775.0
77	10775.0
78	8808.5
79	6842.0
80	6842.0
81	6842.0
82	4738.0
83	2634.0
84	2634.0
85	2634.0
86	2072.0
87	1510.0
88	1510.0
89	1510.0
90	1054.0
91	598.0
92	598.0
93	598.0
94	418.5
95	239.0
96	239.0
97	239.0
98	403.0
99	567.0
100	567.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.03867265702690605
2	0.004775558131651837
3	0.002060044684241969
4	0.00421372776322221
5	0.008802009105397504
6	0.01357756723704934
7	0.02191138436875549
8	0.03492712123737521
9	0.047193750948088746
10	0.057681251158775135
11	0.05431026894819736
12	0.06442321557993068
13	0.06339319323780969
14	0.06601506829048129
15	0.06077131818513809
16	0.0682623897641998
17	0.06208225571147388
18	0.07800078281698
19	0.07369341665901953
20	0.07846897479067136
21	0.07472343900114052
22	0.07603437652747631
23	0.08127812663281951
24	0.07650256850116767
25	0.07528526936957014
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	1067938.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	42.0071566559173
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.42554597108186	32.52427038777162
2	12.902101846851814	10.83961226942608
3	3.870649731183231	4.877849688539945
4	1.6769697909174381	2.817789308572388
5	0.9267124630594061	1.9464277805363726
6	0.5537436874889978	1.3956718696545387
7	0.3804974355601717	1.118853076792565
8	0.2782861506614754	0.9352007940807039
9	0.1980555516554093	0.7487775526466609
>10	1.347157565720868	11.320874540957428
>50	0.21376922371976648	6.3661713537386255
>100	0.20258498262639255	17.06634547417116
>500	0.02034793973891844	5.809919658382834
>1k	0.00357765973431533	2.232236244729008
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTGTAAAGGTATAAGGGAGCTTGAC	2536	0.23746696905625608	No Hit
GTATCAACGCAGAGTACTTTTTTTT	2334	0.21855201331912524	No Hit
CCCTTATACCTTTACACTCTGCGAA	2297	0.21508739271380922	No Hit
CCTTTACACTCTGCGAATGATTTCC	2103	0.1969215441345846	No Hit
GTATAAGGGAGCTTGACTGCGAGAG	1671	0.1564697576076514	No Hit
GTCCTAAGGTAGCGAAATTCCTTGT	1548	0.144952235054844	No Hit
TATCAACGCAGAGTACTTTTTTTTT	1545	0.1446713198706292	No Hit
GTCTCAACGAGAGACTCGGTGAAAT	1274	0.11929531489655767	No Hit
GTCCCAAGGGTTGGGCTGTTCGCCC	1098	0.10281495742262192	No Hit
GTACTTTTTTTTTTTTTTTTTTTTT	1086	0.10169129668576266	No Hit
GGTATAAGGGAGCTTGACTGCGAGA	1082	0.10131674310680956	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	1.8727678947654265E-4
7	0.0	0.0	0.0	0.0	1.8727678947654265E-4
8	0.0	0.0	0.0	0.0	1.8727678947654265E-4
9	0.0	0.0	0.0	0.0	1.8727678947654265E-4
10	0.0	0.0	0.0	0.0	1.8727678947654265E-4
11	0.0	0.0	0.0	0.0	1.8727678947654265E-4
12	0.0	0.0	0.0	0.0	5.61830368429628E-4
13	0.0	0.0	0.0	0.0	6.554687631678993E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CCCTTAT	295	0.0	14.808116	1
CGAATGA	285	0.0	14.668149	14
CTTATAC	285	0.0	14.663339	3
TTATACC	305	0.0	14.637396	4
CGTCGTA	130	0.0	14.616862	10
AGGTATA	365	0.0	14.576817	7
GTGTAAA	445	0.0	14.51152	1
GCGAAAT	250	0.0	14.4407835	12
TGTAAAG	375	0.0	14.434019	2
CCTTATA	310	0.0	14.397261	2
CGAAATT	245	0.0	14.347717	13
CCGTCGT	140	0.0	14.252109	9
TTACAAT	95	4.8876245E-9	14.001415	16
TAGGCCC	190	0.0	13.9981365	4
GTAAAGG	380	0.0	13.996823	3
TTACGCT	75	9.648411E-7	13.935396	15
ACCGTTA	185	0.0	13.866917	8
TTGGGGC	55	1.961461E-4	13.816341	4
GGACCGT	200	0.0	13.774457	6
GACCGTT	190	0.0	13.501365	7
>>END_MODULE