FastQCFastQC Report
Thu 2 Feb 2017
SRR4063996_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4063996_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1067938
Sequences flagged as poor quality0
Sequence length50
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTAAAGGTATAAGGGAGCTTGACTGCGAGAGCTACAACTCGAGCAGGG23820.22304665626656228No Hit
CCCTTATACCTTTACACTCTGCGAATGATTTCCAACCATTCTGAGGGAAC21280.19926250400304138No Hit
CCTTTACACTCTGCGAATGATTTCCAACCATTCTGAGGGAACCTTTGGGC20120.1884004502134019No Hit
GTATAAGGGAGCTTGACTGCGAGAGCTACAACTCGAGCAGGGACGAAAGT16790.15721886476555755No Hit
GTCCTAAGGTAGCGAAATTCCTTGTCGGGTAAGTTCCGACCCGCACGAAA13310.12463270339663914No Hit
GTCTCAACGAGAGACTCGGTGAAATTTTAGTACCTGTGAAGATGCAGGTT12760.1194825916860342No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCATAGG608.018105E-932.9957851
CCTTATA5200.025.3813762
CCCTTAT5300.024.4874381
CGTATAA450.001398384524.44361144
GTAGGAC6000.023.097053
TATACCT5650.022.1918565
GCATACG500.00257724622.00440237
TCCGCTA602.8731357E-421.99925244
TCGATTA500.002581454321.9982220
CGCGTAT500.002581454321.9982242
TATATTG500.002582156321.997195
TAGGACG6000.021.997194
GTATCAA18450.021.937581
TTATACC5750.021.805914
TGTAGGA6500.021.3203542
CCGCACG3750.021.12125841
CGCACGA3650.021.09418542
ACCTTTA6100.020.554758
GATATAC2250.020.530711
ATACGCG550.004481729620.0011939
Produced by FastQC (version 0.11.5)