##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4063996_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1067938
Sequences flagged as poor quality	0
Sequence length	50
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.330765456421627	32.0	32.0	32.0	32.0	32.0
2	31.416130898984772	32.0	32.0	32.0	32.0	32.0
3	31.47664564796833	32.0	32.0	32.0	32.0	32.0
4	31.55472415065294	32.0	32.0	32.0	32.0	32.0
5	31.50859038633329	32.0	32.0	32.0	32.0	32.0
6	35.095543936071195	36.0	36.0	36.0	36.0	36.0
7	35.121611928782386	36.0	36.0	36.0	36.0	36.0
8	35.067517028142085	36.0	36.0	36.0	36.0	36.0
9	35.166088293515166	36.0	36.0	36.0	36.0	36.0
10	35.028551282939645	36.0	36.0	36.0	36.0	36.0
11	35.16464907138804	36.0	36.0	36.0	36.0	36.0
12	35.07190024139978	36.0	36.0	36.0	36.0	36.0
13	35.111288295762485	36.0	36.0	36.0	36.0	36.0
14	35.0592824677088	36.0	36.0	36.0	36.0	36.0
15	35.03086040575389	36.0	36.0	36.0	36.0	36.0
16	35.05161910148342	36.0	36.0	36.0	36.0	36.0
17	35.01267957503151	36.0	36.0	36.0	36.0	36.0
18	35.00598255703983	36.0	36.0	36.0	36.0	36.0
19	35.00943500465383	36.0	36.0	36.0	36.0	36.0
20	34.99183192282698	36.0	36.0	36.0	36.0	36.0
21	34.98831954664035	36.0	36.0	36.0	36.0	36.0
22	34.97194219140062	36.0	36.0	36.0	36.0	36.0
23	34.936519723055085	36.0	36.0	36.0	36.0	36.0
24	34.89902690980188	36.0	36.0	36.0	32.0	36.0
25	34.8665877607127	36.0	36.0	36.0	32.0	36.0
26	34.82252715045255	36.0	36.0	36.0	32.0	36.0
27	34.805565491629665	36.0	36.0	36.0	32.0	36.0
28	34.78227387732247	36.0	36.0	36.0	32.0	36.0
29	34.76032691036371	36.0	36.0	36.0	32.0	36.0
30	34.74052238987657	36.0	36.0	36.0	32.0	36.0
31	34.74124059636421	36.0	36.0	36.0	32.0	36.0
32	34.688967898885515	36.0	36.0	36.0	32.0	36.0
33	34.67435188185082	36.0	36.0	36.0	32.0	36.0
34	34.66539911492989	36.0	36.0	36.0	32.0	36.0
35	34.6113735067017	36.0	36.0	36.0	32.0	36.0
36	34.59747382338675	36.0	36.0	36.0	32.0	36.0
37	34.58022282192412	36.0	36.0	36.0	32.0	36.0
38	34.54443703660699	36.0	36.0	36.0	32.0	36.0
39	34.53764825298847	36.0	36.0	36.0	32.0	36.0
40	34.51592601817708	36.0	36.0	36.0	32.0	36.0
41	34.5103947981999	36.0	36.0	36.0	32.0	36.0
42	34.43457953551611	36.0	36.0	36.0	32.0	36.0
43	34.440526509965935	36.0	36.0	36.0	32.0	36.0
44	34.36292743586238	36.0	36.0	36.0	32.0	36.0
45	34.33666092975435	36.0	36.0	36.0	32.0	36.0
46	34.295398234728985	36.0	36.0	36.0	32.0	36.0
47	34.270545668381494	36.0	36.0	36.0	32.0	36.0
48	34.21908387940124	36.0	36.0	36.0	32.0	36.0
49	34.2052179059084	36.0	36.0	36.0	32.0	36.0
50	33.61350565294989	36.0	36.0	36.0	27.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
15	1.0
16	0.0
17	0.0
18	0.0
19	1.0
20	4.0
21	26.0
22	95.0
23	339.0
24	762.0
25	1842.0
26	3891.0
27	7137.0
28	11972.0
29	18594.0
30	26694.0
31	38559.0
32	55681.0
33	89774.0
34	185640.0
35	626926.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.581649279868145	17.936620905362126	11.512745116401026	26.968984698368697
2	16.233025636415736	19.796541733852465	37.50681221873261	26.463620410999184
3	18.586694333742845	23.713593323944508	29.1449113055464	28.554801036766246
4	12.174489530291083	16.057673760087194	36.918622616668756	34.84921409295296
5	14.16814459266362	36.73181401916591	33.50147667748502	15.598564710685453
6	33.35496382277231	36.97474663767619	16.49245227012455	13.177837269426943
7	29.68880215892683	30.85019916886561	20.1948053164135	19.266193355794062
8	28.223454919667624	33.365232813140835	18.84659970897187	19.56471255821967
9	28.449496406111297	13.418183316415774	17.9699117165641	40.16240856090883
10	16.691559083047654	26.22927179615873	31.020788835212826	26.058380285580785
11	37.9940595802378	20.602319610314456	22.66629710713543	18.737323702312306
12	24.479438316823245	23.751115472599874	29.366261741139194	22.40318446943769
13	29.930435710854518	19.43586348581793	25.08715434395229	25.546546459375257
14	23.607803845739674	20.46182586018812	24.24810498766311	31.682265306409096
15	25.885843085512338	27.20846236612506	22.103492804909912	24.802201743452688
16	25.994050166256372	25.83514447121485	23.671611127706054	24.499194234822728
17	24.019840103077144	25.89654081042158	24.87859782122183	25.20502126527944
18	24.474406706019835	24.96629011476343	26.684183321847005	23.875119857369732
19	26.94894272888501	24.05420539394609	24.451887656399528	24.54496422076937
20	25.493500984614176	24.30859297089419	25.06613254976211	25.131773494729526
21	27.887573997741445	22.533986055370256	24.069093898709475	25.509346048178827
22	25.588213536940852	24.52110939128432	24.79912204558883	25.091555026186
23	24.748110359499893	24.545006779483263	25.757354428388428	24.94952843262842
24	25.401312629163066	25.30617557468493	24.280268518717936	25.01224327743407
25	24.826719554424386	24.669030135159684	25.07617597539895	25.428074335016976
26	24.943018262248966	25.08142254884885	25.390163369498243	24.585395819403942
27	26.43615731172515	24.101128698611344	24.54313130755047	24.919582682113035
28	24.211789008823917	25.08107085634984	25.40703984387949	25.30010029094675
29	25.517366664544138	24.078478670447268	24.57365241200553	25.83050225300307
30	23.797189798622522	25.627706843837643	26.37843608219909	24.19666727534074
31	25.870258202508982	23.857698315484285	25.216604672592624	25.05543880941411
32	25.22937498010117	24.934216810156737	24.152403497699225	25.684004712042864
33	24.476653428686742	24.138883608471684	25.005922911319267	26.378540051522304
34	25.160875883974587	24.45650545367374	25.09588876902793	25.286729893323745
35	25.930066944031644	24.125977262064954	25.62301773469038	24.32093805921302
36	24.430403928597517	25.23181834356874	24.773706714912603	25.564071012921147
37	25.673680100158908	24.40446370129122	24.428342274532426	25.493513924017442
38	25.022381546368194	24.910473814527215	25.266892916942922	24.800251722161665
39	26.463295183622403	24.203435159537708	23.911085141090254	25.42218451574963
40	25.822705867260225	24.999133821327472	24.937143520655784	24.241016790756515
41	25.258613828009047	24.703320988290155	25.387089675579755	24.65097550812104
42	26.38383622727941	25.655222914846536	24.00903064745547	23.951910210418582
43	25.382263569666126	24.208387371654112	25.03064382464897	25.378705234030797
44	24.34746862586828	25.140038841933553	24.643558775111945	25.86893375708622
45	25.222769495842385	24.846055884335904	24.865626638699528	25.065547981122183
46	25.447948123697827	24.609312451717116	24.810543811597256	25.1321956129878
47	25.44680731522319	24.7728741186055	24.493543463400474	25.286775102770832
48	25.59796313758466	25.7213809687457	23.91758910102564	24.763066792643997
49	25.131234701769962	25.23582918045497	24.432970576833398	25.199965540941673
50	24.816279749196575	25.666805252788578	24.1889902690069	25.32792472900795
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	21.0
1	20.5
2	20.0
3	47.5
4	75.0
5	97.0
6	119.0
7	119.5
8	120.0
9	165.5
10	211.0
11	323.5
12	436.0
13	788.0
14	1140.0
15	1646.5
16	2153.0
17	2346.5
18	2540.0
19	2517.5
20	2495.0
21	2697.5
22	2900.0
23	3144.0
24	3388.0
25	3729.5
26	4071.0
27	5269.5
28	6468.0
29	8041.5
30	9615.0
31	11754.5
32	13894.0
33	17565.5
34	21237.0
35	25061.5
36	28886.0
37	33189.5
38	37493.0
39	39693.5
40	41894.0
41	46821.0
42	51748.0
43	55015.5
44	58283.0
45	63890.5
46	69498.0
47	74824.0
48	80150.0
49	84059.5
50	87969.0
51	90884.5
52	93800.0
53	91415.0
54	89030.0
55	87301.5
56	85573.0
57	80661.0
58	75749.0
59	68513.5
60	61278.0
61	52990.5
62	44703.0
63	37169.0
64	29635.0
65	25639.0
66	21643.0
67	17338.0
68	13033.0
69	11709.0
70	10385.0
71	8001.5
72	5618.0
73	4772.0
74	3926.0
75	3106.0
76	2286.0
77	1977.0
78	1668.0
79	1425.0
80	1182.0
81	942.0
82	702.0
83	586.0
84	470.0
85	344.0
86	218.0
87	157.5
88	97.0
89	72.0
90	47.0
91	36.5
92	26.0
93	21.0
94	16.0
95	17.0
96	18.0
97	11.5
98	5.0
99	5.5
100	6.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.007303794789585163
2	3.745535789530853E-4
3	1.8727678947654265E-4
4	0.0
5	0.0
6	9.363839473827133E-5
7	0.0
8	0.0
9	0.0013109375263357986
10	8.427455526444419E-4
11	0.0
12	4.681919736913566E-4
13	4.681919736913566E-4
14	2.80915184214814E-4
15	0.005337388500081465
16	8.427455526444419E-4
17	0.0
18	1.8727678947654265E-4
19	0.0
20	6.554687631678993E-4
21	0.0
22	9.363839473827133E-5
23	9.363839473827132E-4
24	4.681919736913566E-4
25	0.0014982143158123412
26	0.004869196526390109
27	0.006648326026417264
28	0.00421372776322221
29	0.0028091518421481396
30	0.004026450973745667
31	0.008802009105397504
32	0.0033709822105777677
33	0.004775558131651837
34	0.0031837054211012253
35	0.0029027902368864112
36	0.007116518000108621
37	0.003277343815839496
38	0.008802009105397504
39	0.003745535789530853
40	0.0027155134474098684
41	0.0030900670263629536
42	0.0017791295000271553
43	0.0021536830789802403
44	0.0018727678947654265
45	0.0016854911052888838
46	0.0012172991315975272
47	0.002621875052671597
48	0.001966406289503698
49	3.745535789530853E-4
50	9.363839473827132E-4
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	1067938.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	46.37737027145189
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.78411575783628	36.538001080101004
2	12.363114067310683	11.467374376157261
3	3.6944953804406446	5.140229406745928
4	1.501147413468773	2.784770777058943
5	0.823186005661704	1.9088601093425166
6	0.5061747242793329	1.408503156597161
7	0.3529376065926406	1.145782264456684
8	0.23743918144347886	0.8809443867803736
9	0.191186948184209	0.7980073098307153
>10	1.18612114067473	11.029234779274013
>50	0.17730312946610335	5.822348081461452
>100	0.16632856669535045	15.279042392968101
>500	0.014622291507681857	4.488555997146718
>1k	0.0018277864384602322	1.308345882079173
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTGTAAAGGTATAAGGGAGCTTGACTGCGAGAGCTACAACTCGAGCAGGG	2382	0.22304665626656228	No Hit
CCCTTATACCTTTACACTCTGCGAATGATTTCCAACCATTCTGAGGGAAC	2128	0.19926250400304138	No Hit
CCTTTACACTCTGCGAATGATTTCCAACCATTCTGAGGGAACCTTTGGGC	2012	0.1884004502134019	No Hit
GTATAAGGGAGCTTGACTGCGAGAGCTACAACTCGAGCAGGGACGAAAGT	1679	0.15721886476555755	No Hit
GTCCTAAGGTAGCGAAATTCCTTGTCGGGTAAGTTCCGACCCGCACGAAA	1331	0.12463270339663914	No Hit
GTCTCAACGAGAGACTCGGTGAAATTTTAGTACCTGTGAAGATGCAGGTT	1276	0.1194825916860342	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	1.8727678947654265E-4
13	0.0	0.0	0.0	0.0	3.745535789530853E-4
14	0.0	0.0	0.0	0.0	3.745535789530853E-4
15	0.0	0.0	0.0	9.363839473827133E-5	4.681919736913566E-4
16	0.0	0.0	0.0	9.363839473827133E-5	4.681919736913566E-4
17	0.0	0.0	0.0	9.363839473827133E-5	4.681919736913566E-4
18	0.0	0.0	0.0	9.363839473827133E-5	4.681919736913566E-4
19	0.0	0.0	0.0	9.363839473827133E-5	4.681919736913566E-4
20	0.0	0.0	0.0	9.363839473827133E-5	4.681919736913566E-4
21	0.0	0.0	0.0	9.363839473827133E-5	4.681919736913566E-4
22	0.0	0.0	0.0	2.80915184214814E-4	4.681919736913566E-4
23	0.0	0.0	0.0	4.681919736913566E-4	4.681919736913566E-4
24	0.0	0.0	0.0	0.0015918527105506124	4.681919736913566E-4
25	0.0	0.0	0.0	0.001779129500027155	4.681919736913566E-4
26	0.0	0.0	0.0	0.002060044684241969	4.681919736913566E-4
27	0.0	0.0	0.0	0.002340959868456783	4.681919736913566E-4
28	0.0	0.0	0.0	0.003839174184269124	4.681919736913566E-4
29	0.0	0.0	0.0	0.008614732315920961	4.681919736913566E-4
30	0.0	0.0	0.0	0.01685491105288884	4.681919736913566E-4
31	0.0	0.0	0.0	0.03239888457944188	4.681919736913566E-4
32	0.0	0.0	0.0	0.05075200994814306	4.681919736913566E-4
33	0.0	0.0	0.0	0.06938605050105905	4.681919736913566E-4
34	0.0	0.0	0.0	0.09139107326455281	6.554687631678993E-4
35	0.0	0.0	0.0	0.12041897563341693	6.554687631678993E-4
36	0.0	0.0	0.0	0.15796797192346373	6.554687631678993E-4
37	0.0	0.0	0.0	0.21630469184540677	6.554687631678993E-4
38	0.0	0.0	0.0	0.28737623345175467	6.554687631678993E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GCATAGG	60	8.018105E-9	32.995785	1
CCTTATA	520	0.0	25.381376	2
CCCTTAT	530	0.0	24.487438	1
CGTATAA	45	0.0013983845	24.443611	44
GTAGGAC	600	0.0	23.09705	3
TATACCT	565	0.0	22.191856	5
GCATACG	50	0.002577246	22.004402	37
TCCGCTA	60	2.8731357E-4	21.999252	44
TCGATTA	50	0.0025814543	21.99822	20
CGCGTAT	50	0.0025814543	21.99822	42
TATATTG	50	0.0025821563	21.99719	5
TAGGACG	600	0.0	21.99719	4
GTATCAA	1845	0.0	21.93758	1
TTATACC	575	0.0	21.80591	4
TGTAGGA	650	0.0	21.320354	2
CCGCACG	375	0.0	21.121258	41
CGCACGA	365	0.0	21.094185	42
ACCTTTA	610	0.0	20.55475	8
GATATAC	225	0.0	20.53071	1
ATACGCG	55	0.0044817296	20.00119	39
>>END_MODULE