FastQCFastQC Report
Thu 2 Feb 2017
SRR4063995_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4063995_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20305
Sequences flagged as poor quality0
Sequence length25
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGG1680.8273824181236148No Hit
GTATCAACGCAGAGTACTTTTTTTT630.3102684067963556No Hit
TATCAACGCAGAGTACTTTTTTTTT410.2019207091849298No Hit
GTATCAACGCAGAGTACATGGGGGG360.17729623245506032No Hit
GCCCTTGGAAGGGTACCGTTGTCTC280.13789706968726914No Hit
GCTTAAGATTGTCCACATGCAACTA270.13297217434129527No Hit
GTACATGGGACATCTGGCCCCATGT250.12312238364934745No Hit
GTGCTGGAGGGATGCCTGTGAGCTC230.11327259295739965No Hit
GTCCTAGTGTTGGGATTGCGGCATA230.11327259295739965No Hit
GGTATCAACGCAGAGTACTTTTTTT220.10834769761142576No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers