##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4063995_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	20305
Sequences flagged as poor quality	0
Sequence length	25
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.215611918246736	32.0	32.0	32.0	32.0	32.0
2	30.86771731100714	32.0	32.0	32.0	32.0	32.0
3	30.86619059344989	32.0	32.0	32.0	32.0	32.0
4	30.882344250184683	32.0	32.0	32.0	32.0	32.0
5	30.852351637527704	32.0	32.0	32.0	32.0	32.0
6	34.49943363703521	36.0	36.0	36.0	32.0	36.0
7	34.44496429450874	36.0	36.0	36.0	32.0	36.0
8	34.39541984732824	36.0	36.0	36.0	32.0	36.0
9	34.497709923664125	36.0	36.0	36.0	32.0	36.0
10	34.22831814823935	36.0	36.0	36.0	32.0	36.0
11	34.483920216695395	36.0	36.0	36.0	32.0	36.0
12	34.38931297709924	36.0	36.0	36.0	32.0	36.0
13	34.4495444471805	36.0	36.0	36.0	32.0	36.0
14	34.3218419108594	36.0	36.0	36.0	32.0	36.0
15	34.25658704752524	36.0	36.0	36.0	32.0	36.0
16	34.24722974636789	36.0	36.0	36.0	32.0	36.0
17	34.118098990396454	36.0	36.0	36.0	32.0	36.0
18	34.243388327998034	36.0	36.0	36.0	32.0	36.0
19	34.23944841172125	36.0	36.0	36.0	32.0	36.0
20	34.16380201920709	36.0	36.0	36.0	32.0	36.0
21	34.223885742427974	36.0	36.0	36.0	32.0	36.0
22	34.17941393745383	36.0	36.0	36.0	32.0	36.0
23	34.07200196995814	36.0	36.0	36.0	32.0	36.0
24	34.11775424772224	36.0	36.0	36.0	32.0	36.0
25	33.70933267668062	36.0	36.0	36.0	27.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
4	2.0
5	0.0
6	0.0
7	0.0
8	0.0
9	2.0
10	3.0
11	0.0
12	0.0
13	1.0
14	0.0
15	4.0
16	7.0
17	9.0
18	10.0
19	11.0
20	17.0
21	34.0
22	58.0
23	70.0
24	110.0
25	141.0
26	220.0
27	296.0
28	383.0
29	558.0
30	729.0
31	932.0
32	1298.0
33	1867.0
34	3933.0
35	9610.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.919018767548394	18.45721885621398	13.033840697502585	24.58992167873504
2	17.084461955183453	21.004678650578676	36.92194040876631	24.98891898547156
3	19.71629808402699	23.331527360488597	28.64601290449687	28.306161650987537
4	13.480766389203566	16.371964734275725	36.43796483278333	33.70930404373738
5	15.258828744520514	36.442890213268974	33.70930404373738	14.588976998473132
6	33.009900014776136	35.97990444761858	17.268383982662662	13.74181155494262
7	30.740813712934685	30.371391981085605	20.539848290808788	18.34794601517092
8	28.08296792629453	32.32004729763019	20.791249938414545	18.805734837660737
9	27.803508080409934	16.131257390618842	19.22546314544738	36.839771383523846
10	17.226766531979894	26.293485759337738	34.005124667389374	22.474623041292993
11	35.92510470559251	21.729490022172946	23.128849470312886	19.216555801921658
12	24.674748669426375	24.645180366646954	28.700965897890796	21.979105066035874
13	29.62889951209896	21.19658962101424	24.754817406732048	24.419693460154747
14	23.79121691557001	20.24249593375721	26.812558529252307	29.15372862142047
15	25.49154880993446	27.704134430591832	22.234267974178287	24.570048785295423
16	24.592183726775417	28.00256271253265	24.562613966783303	22.84263959390863
17	24.74003252673599	26.469863486274704	25.563057513183185	23.22704647380612
18	23.264984227129336	27.183556782334385	26.45406151419558	23.097397476340696
19	24.99630305121506	26.67718243209937	26.06102430127668	22.26549021540888
20	26.004436775942814	25.388217894996302	26.418535863938867	22.188809465122013
21	25.772738476706923	25.8121764850875	25.757949223564207	22.65713581464136
22	25.335239597712484	25.788799053441135	25.877538946953266	22.99842240189312
23	25.24281417936203	25.898535719568113	25.854163585268452	23.00448651580141
24	25.849233348124045	25.301977025094907	26.14997781393285	22.6988118128482
25	24.486073453290608	25.718511215183632	25.8121764850875	23.983238846438255
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	2.0
1	2.0
2	4.5
3	7.0
4	7.0
5	7.0
6	10.0
7	13.0
8	13.0
9	13.0
10	17.0
11	21.0
12	21.0
13	21.0
14	36.5
15	52.0
16	52.0
17	52.0
18	115.5
19	179.0
20	179.0
21	179.0
22	243.5
23	308.0
24	308.0
25	308.0
26	419.0
27	530.0
28	530.0
29	530.0
30	720.0
31	910.0
32	910.0
33	910.0
34	1123.0
35	1336.0
36	1336.0
37	1336.0
38	1788.0
39	2240.0
40	2240.0
41	2240.0
42	2463.5
43	2687.0
44	2687.0
45	2687.0
46	2837.5
47	2988.0
48	2988.0
49	2988.0
50	2757.5
51	2527.0
52	2527.0
53	2527.0
54	2452.5
55	2378.0
56	2378.0
57	2378.0
58	2086.0
59	1794.0
60	1794.0
61	1794.0
62	1431.5
63	1069.0
64	1069.0
65	1069.0
66	837.5
67	606.0
68	606.0
69	606.0
70	440.5
71	275.0
72	275.0
73	275.0
74	200.0
75	125.0
76	125.0
77	125.0
78	89.5
79	54.0
80	54.0
81	54.0
82	35.0
83	16.0
84	16.0
85	16.0
86	11.5
87	7.0
88	7.0
89	7.0
90	6.0
91	5.0
92	5.0
93	5.0
94	3.5
95	2.0
96	2.0
97	2.0
98	88.0
99	174.0
100	174.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.01969958138389559
2	0.0
3	0.009849790691947795
4	0.009849790691947795
5	0.009849790691947795
6	0.009849790691947795
7	0.014774686037921694
8	0.03939916276779118
9	0.04432405811376508
10	0.05417384880571288
11	0.04924895345973898
12	0.06402363949766067
13	0.06894853484363457
14	0.07879832553558236
15	0.059098744151686776
16	0.06894853484363457
17	0.06894853484363457
18	0.08372322088155626
19	0.08864811622753016
20	0.09849790691947796
21	0.09849790691947796
22	0.10342280226545185
23	0.10834769761142576
24	0.10834769761142576
25	0.09849790691947796
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	20305.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	51.85914799310515
#Duplication Level	Percentage of deduplicated	Percentage of total
1	64.78632478632478	33.59763605023393
2	16.533713200379868	17.148485594681112
3	7.578347578347579	11.790199458261512
4	3.903133903133903	8.09652794878109
5	2.4976258309591644	6.476237379955677
6	1.3200379867046534	4.107362718542231
7	1.00664767331434	3.654272346712632
8	0.5982905982905984	2.4821472543708447
9	0.4748338081671415	2.216202905688254
>10	1.282051282051282	9.293277517852745
>50	0.00949667616334283	0.3102684067963556
>100	0.00949667616334283	0.8273824181236148
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GGGGGGGGGGGGGGGGGGGGGGGGG	168	0.8273824181236148	No Hit
GTATCAACGCAGAGTACTTTTTTTT	63	0.3102684067963556	No Hit
TATCAACGCAGAGTACTTTTTTTTT	41	0.2019207091849298	No Hit
GTATCAACGCAGAGTACATGGGGGG	36	0.17729623245506032	No Hit
GCCCTTGGAAGGGTACCGTTGTCTC	28	0.13789706968726914	No Hit
GCTTAAGATTGTCCACATGCAACTA	27	0.13297217434129527	No Hit
GTACATGGGACATCTGGCCCCATGT	25	0.12312238364934745	No Hit
GTGCTGGAGGGATGCCTGTGAGCTC	23	0.11327259295739965	No Hit
GTCCTAGTGTTGGGATTGCGGCATA	23	0.11327259295739965	No Hit
GGTATCAACGCAGAGTACTTTTTTT	22	0.10834769761142576	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	pass
>>END_MODULE