FastQCFastQC Report
Thu 2 Feb 2017
SRR4063995_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4063995_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20305
Sequences flagged as poor quality0
Sequence length50
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACATGCGCAGATCTCGTATGCCGT350.17237133710908645No Hit
ACACATCTCCGAGCCCACGAGACATGCGCAGATCTCGTATGCCGTCTTCT260.12804727899532134RNA PCR Primer, Index 47 (95% over 24bp)
GTACATGGGACATCTGGCCCCATGTACCAGCAAAGGGCCTGAGAAGTATA260.12804727899532134No Hit
ATACACATCTCCGAGCCCACGAGACATGCGCAGATCTCGTATGCCGTCTT250.12312238364934745RNA PCR Primer, Index 47 (95% over 22bp)
CACTAGGACTGAGGGTTACAAGTTTGAGATCAGCCTGAGTAACTTAGTGA240.11819748830337355No Hit
GATATAGACTGTGCTGACTGGAAGCCCAAGACCTGAGGCAAGATGTGTCC230.11327259295739965No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTAGTA250.002298892235.231
GTATCAA401.7339506E-533.01
CAGGGCT300.005614652829.3333324
TATCAAC507.951923E-526.42
AACGCAG654.7064223E-420.3076926
CAACGCA654.7064223E-420.3076925
TCAACGC707.754016E-418.8571434
ATCAACG707.754016E-418.8571433
ACGCAGA800.00189916916.57
AGAGTAC800.00189916916.511
CGCAGAG800.00189916916.58
CAGAGTA850.002847188915.52941210
GCAGAGT900.004166139814.6666689
Produced by FastQC (version 0.11.5)