##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4063995_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	20305
Sequences flagged as poor quality	0
Sequence length	50
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.338685052942626	32.0	32.0	32.0	32.0	32.0
2	31.436641221374046	32.0	32.0	32.0	32.0	32.0
3	31.48135927111549	32.0	32.0	32.0	32.0	32.0
4	31.58857424279734	32.0	32.0	32.0	32.0	32.0
5	31.537010588524993	32.0	32.0	32.0	32.0	32.0
6	35.10746121644915	36.0	36.0	36.0	36.0	36.0
7	35.10716572272839	36.0	36.0	36.0	36.0	36.0
8	35.084018714602315	36.0	36.0	36.0	36.0	36.0
9	35.119379463186405	36.0	36.0	36.0	36.0	36.0
10	35.03186407288845	36.0	36.0	36.0	36.0	36.0
11	35.19812853976853	36.0	36.0	36.0	36.0	36.0
12	35.09711893622261	36.0	36.0	36.0	36.0	36.0
13	35.1244028564393	36.0	36.0	36.0	36.0	36.0
14	35.05048017729623	36.0	36.0	36.0	36.0	36.0
15	35.035508495444475	36.0	36.0	36.0	36.0	36.0
16	35.010933267668065	36.0	36.0	36.0	36.0	36.0
17	35.04535828613642	36.0	36.0	36.0	36.0	36.0
18	35.009751292785026	36.0	36.0	36.0	36.0	36.0
19	34.98872198965772	36.0	36.0	36.0	36.0	36.0
20	34.99876877616351	36.0	36.0	36.0	36.0	36.0
21	34.962078305836	36.0	36.0	36.0	36.0	36.0
22	34.983452351637524	36.0	36.0	36.0	36.0	36.0
23	34.90199458261512	36.0	36.0	36.0	32.0	36.0
24	34.94449642945087	36.0	36.0	36.0	36.0	36.0
25	34.87146023147008	36.0	36.0	36.0	32.0	36.0
26	34.87909381925634	36.0	36.0	36.0	32.0	36.0
27	34.82999261265698	36.0	36.0	36.0	32.0	36.0
28	34.80344742674218	36.0	36.0	36.0	32.0	36.0
29	34.79295739965526	36.0	36.0	36.0	32.0	36.0
30	34.779906426988425	36.0	36.0	36.0	32.0	36.0
31	34.75065254863334	36.0	36.0	36.0	32.0	36.0
32	34.73341541492243	36.0	36.0	36.0	32.0	36.0
33	34.655257325781825	36.0	36.0	36.0	32.0	36.0
34	34.59960600837232	36.0	36.0	36.0	32.0	36.0
35	34.657178034966755	36.0	36.0	36.0	32.0	36.0
36	34.57197734548141	36.0	36.0	36.0	32.0	36.0
37	34.56503324304359	36.0	36.0	36.0	32.0	36.0
38	34.551440531888694	36.0	36.0	36.0	32.0	36.0
39	34.514257572026594	36.0	36.0	36.0	32.0	36.0
40	34.507165722728395	36.0	36.0	36.0	32.0	36.0
41	34.519724205860626	36.0	36.0	36.0	32.0	36.0
42	34.489682344250184	36.0	36.0	36.0	32.0	36.0
43	34.44841172125092	36.0	36.0	36.0	32.0	36.0
44	34.38749076582123	36.0	36.0	36.0	32.0	36.0
45	34.45407535089879	36.0	36.0	36.0	32.0	36.0
46	34.304457030288106	36.0	36.0	36.0	32.0	36.0
47	34.240827382418125	36.0	36.0	36.0	32.0	36.0
48	34.255454321595664	36.0	36.0	36.0	32.0	36.0
49	34.193154395469094	36.0	36.0	36.0	32.0	36.0
50	33.622408273824185	36.0	36.0	36.0	27.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	1.0
19	0.0
20	0.0
21	2.0
22	3.0
23	7.0
24	21.0
25	32.0
26	76.0
27	147.0
28	244.0
29	412.0
30	500.0
31	750.0
32	1025.0
33	1635.0
34	3080.0
35	12370.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.73561859732072	19.483845547675337	13.337273443656422	25.44326241134752
2	16.18320610687023	21.26569810391529	37.43905442009357	25.11204136912091
3	17.71484855946811	24.56045309037183	30.115735040630387	27.60896330952967
4	12.459985225313961	17.690224082738244	36.025609455799064	33.82418123614873
5	13.64688500369367	36.78896823442502	34.00147746860379	15.562669293277517
6	32.13986702782566	37.2075843388328	17.217434129524747	13.435114503816795
7	29.91873922679143	31.13026348190101	19.82270376754494	19.128293523762622
8	26.865304112287614	33.31691701551342	21.063777394730362	18.754001477468606
9	27.205121891159813	15.99606008372322	18.68505294262497	38.113765082491994
10	16.104407781334647	27.34794385619306	34.16892391036691	22.37872445210539
11	35.62669293277518	22.462447672986947	23.201181974883035	18.70967741935484
12	23.93006648608717	25.23516375277025	28.574242797340553	22.26052696380202
13	28.948534843634572	21.245998522531394	25.087416892391033	24.718049741442993
14	23.565624230485103	20.408766313715834	26.614134449642947	29.411475006156117
15	25.315208825847122	27.506895193065407	22.0596926713948	25.11820330969267
16	24.796848066978576	27.318394484117213	24.191085939423786	23.693671509480424
17	23.63949766067471	27.633587786259543	25.264713124846093	23.46220142821965
18	22.6791430682098	27.810884018714603	26.372814577690225	23.137158335385376
19	24.860871706476235	26.25461708938685	26.195518345235165	22.68899285890175
20	25.285657998423954	25.295508274231675	26.191883372734438	23.22695035460993
21	25.574981531642454	25.37306082245752	25.983747845358284	23.068209800541737
22	24.663875892637282	26.5845850775671	26.195518345235165	22.556020684560455
23	24.44718049741443	25.944348682590494	26.87022900763359	22.738241812361487
24	25.48140852006895	25.993597636050236	25.417384880571287	23.107608963309527
25	24.731553541523002	25.204413358289823	26.568810954585754	23.495222145601417
26	24.70570851598286	25.59227700339851	26.109441954390977	23.592572526227652
27	23.833916170024132	26.912278973550706	25.513470915628233	23.740333940796926
28	25.04063438900655	26.07988967147712	25.799142983795498	23.080332955720827
29	24.325256107171	26.457840819542948	25.59594168636722	23.620961386918836
30	24.38675992513053	26.657472170229536	26.5786622007684	22.37710570387154
31	24.49019801004827	25.44576888976455	26.307752930745735	23.756280169441435
32	23.636900950598434	27.16839875880412	24.95690292075063	24.23779736984682
33	23.47783251231527	25.615763546798032	26.290640394088673	24.615763546798032
34	23.443656422379828	26.221434200157606	26.083530338849485	24.25137903861308
35	22.991676106979263	26.78914446140964	26.956607397921488	23.2625720336896
36	25.58368633632154	25.524578859225695	25.88907496798345	23.002659836469313
37	25.09604965028076	25.672347551965323	25.958033691261946	23.27356910649197
38	24.625615763546797	24.822660098522167	26.88177339901478	23.669950738916256
39	23.34745345286179	25.652645059600037	25.80533937543099	25.19456211210718
40	23.385706545830665	26.109441954390977	27.188100280746685	23.31675121903167
41	24.695857755011573	26.375412500615674	25.46914249125745	23.459587253115302
42	24.605988967691093	25.75847123719464	25.87174940898345	23.76379038613081
43	24.67001576044129	25.438337273443658	26.5908195429472	23.30082742316785
44	23.2625720336896	25.656306949711865	26.670935329754226	24.410185686844308
45	23.12352245862884	25.162529550827422	27.433018124507484	24.28092986603625
46	24.21197793538219	25.216706067769895	26.54156816390859	24.02974783293932
47	23.659197242058603	25.579906426988426	27.495690716572273	23.265205614380694
48	23.18262411347518	25.04432624113475	27.654649330181247	24.118400315208827
49	23.64066193853428	25.201930654058312	27.132584712371948	24.02482269503546
50	23.256501182033094	25.940701339637513	26.787825059101657	24.01497241922774
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	2.0
1	1.5
2	1.0
3	3.0
4	5.0
5	6.5
6	8.0
7	8.0
8	8.0
9	11.5
10	15.0
11	12.0
12	9.0
13	27.5
14	46.0
15	50.5
16	55.0
17	49.5
18	44.0
19	42.0
20	40.0
21	54.5
22	69.0
23	82.0
24	95.0
25	132.0
26	169.0
27	167.5
28	166.0
29	237.5
30	309.0
31	346.5
32	384.0
33	425.0
34	466.0
35	528.0
36	590.0
37	678.0
38	766.0
39	922.5
40	1079.0
41	1187.5
42	1296.0
43	1385.5
44	1475.0
45	1640.5
46	1806.0
47	1757.5
48	1709.0
49	1678.0
50	1647.0
51	1660.0
52	1673.0
53	1614.5
54	1556.0
55	1444.0
56	1332.0
57	1201.0
58	1070.0
59	953.0
60	836.0
61	687.0
62	538.0
63	439.0
64	340.0
65	287.0
66	234.0
67	200.0
68	166.0
69	141.0
70	116.0
71	99.0
72	82.0
73	64.5
74	47.0
75	36.0
76	25.0
77	21.0
78	17.0
79	10.5
80	4.0
81	4.0
82	4.0
83	3.0
84	2.0
85	1.5
86	1.0
87	0.5
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.5
94	1.0
95	1.0
96	1.0
97	0.5
98	0.0
99	0.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.004924895345973897
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.004924895345973897
16	0.0
17	0.0
18	0.0
19	0.0
20	0.004924895345973897
21	0.0
22	0.0
23	0.0
24	0.0
25	0.014774686037921694
26	0.009849790691947795
27	0.009849790691947795
28	0.009849790691947795
29	0.004924895345973897
30	0.014774686037921694
31	0.014774686037921694
32	0.009849790691947795
33	0.02462447672986949
34	0.004924895345973897
35	0.009849790691947795
36	0.014774686037921694
37	0.014774686037921694
38	0.02462447672986949
39	0.014774686037921694
40	0.009849790691947795
41	0.009849790691947795
42	0.004924895345973897
43	0.004924895345973897
44	0.009849790691947795
45	0.004924895345973897
46	0.004924895345973897
47	0.0
48	0.004924895345973897
49	0.004924895345973897
50	0.004924895345973897
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	20305.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	55.144053188869734
#Duplication Level	Percentage of deduplicated	Percentage of total
1	67.64311869250692	37.30115735040631
2	15.682772171117263	17.296232455060327
3	6.778601411092257	11.213986702782567
4	3.3312494418147716	7.347943856193057
5	2.0898454943288383	5.76212755478946
6	1.500401893364294	4.964294508741689
7	0.8395105831919264	3.2405811376508247
8	0.6162364919174779	2.718542230977592
9	0.31258372778422794	1.5513420339817778
>10	1.205680092882022	8.6037921694164
>50	0.0	0.0
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CTTATACACATCTCCGAGCCCACGAGACATGCGCAGATCTCGTATGCCGT	35	0.17237133710908645	No Hit
ACACATCTCCGAGCCCACGAGACATGCGCAGATCTCGTATGCCGTCTTCT	26	0.12804727899532134	RNA PCR Primer, Index 47 (95% over 24bp)
GTACATGGGACATCTGGCCCCATGTACCAGCAAAGGGCCTGAGAAGTATA	26	0.12804727899532134	No Hit
ATACACATCTCCGAGCCCACGAGACATGCGCAGATCTCGTATGCCGTCTT	25	0.12312238364934745	RNA PCR Primer, Index 47 (95% over 22bp)
CACTAGGACTGAGGGTTACAAGTTTGAGATCAGCCTGAGTAACTTAGTGA	24	0.11819748830337355	No Hit
GATATAGACTGTGCTGACTGGAAGCCCAAGACCTGAGGCAAGATGTGTCC	23	0.11327259295739965	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.0	0.0	0.0	0.0	0.0
15	0.0	0.0	0.0	0.0	0.0
16	0.0	0.0	0.0	0.0	0.0
17	0.0	0.0	0.0	0.0	0.0
18	0.0	0.0	0.0	0.0	0.0
19	0.0	0.0	0.0	0.0	0.0
20	0.0	0.0	0.0	0.0	0.0
21	0.0	0.0	0.0	0.0	0.0
22	0.0	0.0	0.0	0.0	0.0
23	0.0	0.0	0.0	0.0	0.0
24	0.0	0.0	0.0	0.0	0.0
25	0.0	0.0	0.0	0.0	0.0
26	0.0	0.0	0.0	0.0	0.0
27	0.0	0.0	0.0	0.004924895345973898	0.0
28	0.0	0.0	0.0	0.004924895345973898	0.0
29	0.0	0.0	0.0	0.014774686037921694	0.0
30	0.0	0.0	0.0	0.02462447672986949	0.0
31	0.0	0.0	0.0	0.10834769761142576	0.0
32	0.0	0.0	0.0	0.17729623245506032	0.0
33	0.0	0.0	0.0	0.241319871952721	0.0
34	0.0	0.0	0.0	0.2954937207584339	0.0
35	0.0	0.0	0.0	0.34474267421817284	0.0
36	0.0	0.0	0.0	0.4432405811376508	0.0
37	0.0	0.0	0.0	0.6205368135927112	0.0
38	0.0	0.0	0.0	0.7978330460477715	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTTAGTA	25	0.0022988922	35.2	31
GTATCAA	40	1.7339506E-5	33.0	1
CAGGGCT	30	0.0056146528	29.333332	4
TATCAAC	50	7.951923E-5	26.4	2
AACGCAG	65	4.7064223E-4	20.307692	6
CAACGCA	65	4.7064223E-4	20.307692	5
TCAACGC	70	7.754016E-4	18.857143	4
ATCAACG	70	7.754016E-4	18.857143	3
ACGCAGA	80	0.001899169	16.5	7
AGAGTAC	80	0.001899169	16.5	11
CGCAGAG	80	0.001899169	16.5	8
CAGAGTA	85	0.0028471889	15.529412	10
GCAGAGT	90	0.0041661398	14.666668	9
>>END_MODULE