FastQCFastQC Report
Thu 2 Feb 2017
SRR4063994_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4063994_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1060161
Sequences flagged as poor quality0
Sequence length25
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT24980.23562458909542985No Hit
TATCAACGCAGAGTACTTTTTTTTT17550.16554089426040006No Hit
GTACTTTTTTTTTTTTTTTTTTTTT11750.1108322226529744No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTAAC250.006024522219.00344711
TCGCGTA250.006027809319.0016529
CGTTATT551.1337808E-515.5365372
AGACCGT456.769883E-414.7748786
TAATCTC400.00528451314.2472035
GCGTTAT655.4738615E-513.1475411
AGGCCCG1950.012.66657310
CGTCGTA1051.9908839E-812.66657310
CGGTCCA1650.012.66657310
CATAATA604.109961E-412.6594012
CAAGACG1301.9826984E-1012.418884
GTATCAA15550.012.3959851
CGAACGA701.0879432E-412.21707916
ACGAACG701.0879432E-412.21707915
CGAGCCG2100.012.21707815
AAGACGG1405.2750693E-1112.2118895
TAAGGTG701.09226734E-412.2118895
CCGTCGT1103.8036887E-812.0919619
GTCTAGT550.003078370612.0851141
TTAGAAC951.0429703E-611.9959473
Produced by FastQC (version 0.11.5)