##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4063994_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1060161
Sequences flagged as poor quality	0
Sequence length	25
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.2696750776533	32.0	32.0	32.0	32.0	32.0
2	30.908487484448116	32.0	32.0	32.0	32.0	32.0
3	30.932141438894657	32.0	32.0	32.0	32.0	32.0
4	30.944550874819956	32.0	32.0	32.0	32.0	32.0
5	30.896112948882294	32.0	32.0	32.0	32.0	32.0
6	34.53857385812155	36.0	36.0	36.0	32.0	36.0
7	34.469796568634386	36.0	36.0	36.0	32.0	36.0
8	34.442236603685664	36.0	36.0	36.0	32.0	36.0
9	34.550628630934355	36.0	36.0	36.0	32.0	36.0
10	34.29183869242502	36.0	36.0	36.0	32.0	36.0
11	34.52664736771113	36.0	36.0	36.0	32.0	36.0
12	34.36709141347399	36.0	36.0	36.0	32.0	36.0
13	34.43918706687003	36.0	36.0	36.0	32.0	36.0
14	34.34196126814701	36.0	36.0	36.0	32.0	36.0
15	34.30326242900843	36.0	36.0	36.0	32.0	36.0
16	34.29686245768331	36.0	36.0	36.0	32.0	36.0
17	34.2335833896927	36.0	36.0	36.0	32.0	36.0
18	34.23322872658021	36.0	36.0	36.0	32.0	36.0
19	34.23697344082644	36.0	36.0	36.0	32.0	36.0
20	34.21272901002772	36.0	36.0	36.0	32.0	36.0
21	34.191161531125935	36.0	36.0	36.0	32.0	36.0
22	34.17409619859625	36.0	36.0	36.0	32.0	36.0
23	34.12209749273931	36.0	36.0	36.0	32.0	36.0
24	34.11714541470588	36.0	36.0	36.0	32.0	36.0
25	33.73184921912804	36.0	36.0	36.0	27.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
3	1.0
4	3.0
5	16.0
6	27.0
7	13.0
8	58.0
9	73.0
10	105.0
11	37.0
12	58.0
13	59.0
14	86.0
15	162.0
16	287.0
17	386.0
18	484.0
19	685.0
20	916.0
21	1432.0
22	2123.0
23	3411.0
24	5253.0
25	7623.0
26	10977.0
27	14830.0
28	19919.0
29	27481.0
30	36118.0
31	49295.0
32	69450.0
33	99367.0
34	215954.0
35	493472.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.635967060248944	17.737151958994218	11.65988678493951	25.96699419581733
2	15.980269804480892	19.883765792127353	38.68706849630365	25.448895907088097
3	18.582188453575185	24.31125637288887	29.0702774594281	28.036277714107843
4	12.218407176478356	15.91389735221152	37.0288549517512	34.83884051955892
5	13.94114622884014	37.21031804053448	34.312720206398545	14.53581552422683
6	33.10969460672822	36.41654159312405	17.465989587214857	13.00777421293287
7	29.466340234634814	30.85641236101873	21.39441197420621	18.282835430140242
8	27.212355562097684	33.925238583201704	19.901622762073526	18.960783092627086
9	27.44110111309813	14.16791314154135	18.9645686567798	39.42641708858072
10	15.611380293812866	27.046978725653226	32.58782203615374	24.753818944380164
11	36.288547710636884	21.150985847436193	23.68242235720029	18.878044084726632
12	24.705920106919265	23.797276239250735	29.777883908405073	21.718919745424927
13	29.475247029464203	20.16170059339927	25.40772013678292	24.955332240353602
14	22.912383832548976	20.446509643573812	26.0297207450574	30.611385778819816
15	24.396005776146026	28.674695383802252	22.551791832227497	24.377507007824224
16	24.740390042606048	25.93437875776093	25.157578194462538	24.16765300517049
17	23.290823357080026	26.528244972232606	26.033113232834616	24.147818437852756
18	23.74879646585739	25.392116143404632	27.58585210217297	23.273235288565008
19	25.210824893100874	25.094061789109034	25.956334186009194	23.738779131780895
20	25.556698792678667	24.876200005663744	25.654209577390336	23.912891624267253
21	26.0493263129676	24.60104550337268	24.86836814254187	24.481260041117846
22	25.418479456205617	25.342396573060803	25.2880246863469	23.95109928438668
23	23.66249355719979	25.285135209124327	26.18221843783099	24.870152795844888
24	24.485124346081015	25.349968189000755	26.089833506704853	24.075073958213373
25	24.26404094524461	25.24764812161236	26.12317178387828	24.36513914926475
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	166.0
1	166.0
2	291.5
3	417.0
4	417.0
5	417.0
6	1113.0
7	1809.0
8	1809.0
9	1809.0
10	1917.5
11	2026.0
12	2026.0
13	2026.0
14	2520.0
15	3014.0
16	3014.0
17	3014.0
18	5305.5
19	7597.0
20	7597.0
21	7597.0
22	12606.0
23	17615.0
24	17615.0
25	17615.0
26	26622.0
27	35629.0
28	35629.0
29	35629.0
30	45652.0
31	55675.0
32	55675.0
33	55675.0
34	66513.0
35	77351.0
36	77351.0
37	77351.0
38	86278.5
39	95206.0
40	95206.0
41	95206.0
42	106307.0
43	117408.0
44	117408.0
45	117408.0
46	128873.0
47	140338.0
48	140338.0
49	140338.0
50	140262.0
51	140186.0
52	140186.0
53	140186.0
54	128503.0
55	116820.0
56	116820.0
57	116820.0
58	105744.0
59	94668.0
60	94668.0
61	94668.0
62	81503.5
63	68339.0
64	68339.0
65	68339.0
66	55381.0
67	42423.0
68	42423.0
69	42423.0
70	31805.0
71	21187.0
72	21187.0
73	21187.0
74	15991.5
75	10796.0
76	10796.0
77	10796.0
78	8627.0
79	6458.0
80	6458.0
81	6458.0
82	4442.5
83	2427.0
84	2427.0
85	2427.0
86	1850.5
87	1274.0
88	1274.0
89	1274.0
90	923.0
91	572.0
92	572.0
93	572.0
94	398.5
95	225.0
96	225.0
97	225.0
98	380.0
99	535.0
100	535.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.03848472071694771
2	0.00565951775249231
3	5.65951775249231E-4
4	0.002924084172121027
5	0.00716872248649026
6	0.011130384913234877
7	0.020374263908972318
8	0.034240082402578476
9	0.04772859971268515
10	0.05423704512805131
11	0.053293792169302585
12	0.06319794823616412
13	0.06338659882791386
14	0.06593338181653542
15	0.05951926169704412
16	0.06480147826603695
17	0.060839815839292335
18	0.0736680560782749
19	0.07083829720202875
20	0.07461130903702362
21	0.0723475019360267
22	0.07451698374114875
23	0.07913892323901747
24	0.07347940548652517
25	0.07498861022052311
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	1060161.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	59.10373352336398
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.3176075373257	46.87966739596864
2	13.140578799022542	15.533145353603892
3	3.597876455346774	6.379437938004033
4	1.412941914142371	3.3404056950985
5	0.6821890244832162	2.0159959157809815
6	0.4069445584407345	1.4431165644518513
7	0.26687893219771314	1.1041478904129491
8	0.1904174320918841	0.9003504931649581
9	0.13376033039973056	0.7115161431548517
>10	0.7013502346344082	7.529233448884385
>50	0.07096752351431854	2.9361115999624134
>100	0.07206703396534835	8.244511710447568
>500	0.005778201991759378	2.3688691341988175
>1k	6.420224435288198E-4	0.6134907168661148
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	2498	0.23562458909542985	No Hit
TATCAACGCAGAGTACTTTTTTTTT	1755	0.16554089426040006	No Hit
GTACTTTTTTTTTTTTTTTTTTTTT	1175	0.1108322226529744	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	9.432529587487183E-5
3	0.0	0.0	0.0	0.0	9.432529587487183E-5
4	0.0	0.0	0.0	0.0	9.432529587487183E-5
5	0.0	0.0	0.0	0.0	9.432529587487183E-5
6	0.0	0.0	0.0	0.0	9.432529587487183E-5
7	0.0	0.0	0.0	0.0	9.432529587487183E-5
8	0.0	0.0	0.0	0.0	9.432529587487183E-5
9	0.0	0.0	0.0	0.0	2.829758876246155E-4
10	0.0	0.0	0.0	0.0	3.7730118349948733E-4
11	0.0	0.0	0.0	0.0	3.7730118349948733E-4
12	0.0	0.0	0.0	0.0	5.65951775249231E-4
13	0.0	0.0	0.0	0.0	6.602770711241028E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GCGTAAC	25	0.0060245222	19.003447	11
TCGCGTA	25	0.0060278093	19.001652	9
CGTTATT	55	1.1337808E-5	15.536537	2
AGACCGT	45	6.769883E-4	14.774878	6
TAATCTC	40	0.005284513	14.247203	5
GCGTTAT	65	5.4738615E-5	13.147541	1
AGGCCCG	195	0.0	12.666573	10
CGTCGTA	105	1.9908839E-8	12.666573	10
CGGTCCA	165	0.0	12.666573	10
CATAATA	60	4.109961E-4	12.659401	2
CAAGACG	130	1.9826984E-10	12.41888	4
GTATCAA	1555	0.0	12.395985	1
CGAACGA	70	1.0879432E-4	12.217079	16
ACGAACG	70	1.0879432E-4	12.217079	15
CGAGCCG	210	0.0	12.217078	15
AAGACGG	140	5.2750693E-11	12.211889	5
TAAGGTG	70	1.09226734E-4	12.211889	5
CCGTCGT	110	3.8036887E-8	12.091961	9
GTCTAGT	55	0.0030783706	12.085114	1
TTAGAAC	95	1.0429703E-6	11.995947	3
>>END_MODULE