FastQCFastQC Report
Thu 2 Feb 2017
SRR4063994_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4063994_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1060161
Sequences flagged as poor quality0
Sequence length50
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTAAAC801.3081808E-724.75257332
TAACGGC1301.8189894E-1223.6947736
ATTGTCG904.080448E-722.00021243
TAGGACG7650.021.2792434
TTAACGG1451.2732926E-1121.24358735
GTATTAG2800.021.214491
GTATCAA18950.021.1294921
GGACGTG8250.020.5315956
AGTCGAA550.00448545119.99830815
TTAGGAC5350.019.3254683
CGCGGGA2050.019.31725944
AGGACGT8800.019.2483715
GACGTGA5200.019.036857
TGTAGGA9100.018.372072
GTAAACG2400.018.33437527
TAGACAG1852.1827873E-1117.8363275
GTAGGAC9250.017.8363273
CGCAATA2650.017.43577636
CGCGGTT3700.017.24178110
GGTATCA10350.017.0049951
Produced by FastQC (version 0.11.5)