FastQCFastQC Report
Thu 2 Feb 2017
SRR4063993_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4063993_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2045491
Sequences flagged as poor quality0
Sequence length25
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT44770.218871654776286No Hit
GTTCAAAGCAGGCCCGAGCCGCCTG29420.1438285477667709No Hit
TATCAACGCAGAGTACTTTTTTTTT28440.13903752204238495No Hit
GATTAAGAGGGACGGCCGGGGGCAT27380.13385539217723275No Hit
GAATAGGACCGCGGTTCTATTTTGT26570.12989546275197494No Hit
GTATCTGATCGTCTTCGAACCTCCG25600.12515332504518473No Hit
GTCTTGCGCCGGTCCAAGAATTTCA25440.12437111676365234No Hit
CTATTGGAGCTGGAATTACCGCGGC24770.1210956195847354No Hit
GCGCAAGACGGACCAGAGCGAAAGC24510.11982453112724524No Hit
GAACTACGACGGTATCTGATCGTCT23480.11478906531488038No Hit
GAATAACGCCGCCGCATCGCCAGTC23350.1141535210861353No Hit
GTCCTATTCCATTATTCCTAGCTGC23020.11254021650547472No Hit
GGGTAGGCACACGCTGAGCCAGTCA22760.11126912804798456No Hit
ATCAGATACCGTCGTAGTTCCGACC22660.11078024787202681No Hit
GTACATGGGGAATAATTGCAATCCC22300.1090202792385789No Hit
GTCCTAAAGTGTGTATTTCTCATTT22290.10897139122098314No Hit
TCGTAGTTCCGACCATAAACGATGC21960.10735808664032254No Hit
CTGTAGGACGTGGAATATGGCAAGA20470.10007377201855201No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTAAGGT954.9258233E-913.99572854
CGCCAGT4450.013.45191518
ATTGCGA500.00149830213.30179810
CGCATCG4600.013.011991513
CGAACGA1850.012.84020116
GCGGGTA604.0866522E-412.66918
CCGCATC5100.012.1094812
GTGCGAA550.003067031712.09195211
TCGGGAC550.003077726612.0863343
CCTAACC550.003077726612.0863343
GTATAGA1501.4551915E-1112.0287791
TCGCCAG5250.011.94505717
GTCTAAA1603.6379788E-1211.8705051
CGACCAT3950.011.78640410
CGTTATT2100.011.7565912
CTCGTAG1800.011.61268110
ACTCTAA4750.011.60156910
GCGTTAT2300.011.5608411
GTATCAA30650.011.55681
CGTCGTA3700.011.55561610
Produced by FastQC (version 0.11.5)