##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4063993_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2045491
Sequences flagged as poor quality	0
Sequence length	25
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.275978725890262	32.0	32.0	32.0	32.0	32.0
2	30.885515507034743	32.0	32.0	32.0	32.0	32.0
3	30.902452760730796	32.0	32.0	32.0	32.0	32.0
4	30.931270291582802	32.0	32.0	32.0	32.0	32.0
5	30.841765131208106	32.0	32.0	32.0	32.0	32.0
6	34.50227255949794	36.0	36.0	36.0	32.0	36.0
7	34.445693478974	36.0	36.0	36.0	32.0	36.0
8	34.412803576256266	36.0	36.0	36.0	32.0	36.0
9	34.54087600483209	36.0	36.0	36.0	32.0	36.0
10	34.24391551955007	36.0	36.0	36.0	32.0	36.0
11	34.50588342847757	36.0	36.0	36.0	32.0	36.0
12	34.317521318842275	36.0	36.0	36.0	32.0	36.0
13	34.41101867473384	36.0	36.0	36.0	32.0	36.0
14	34.30596712476369	36.0	36.0	36.0	32.0	36.0
15	34.24413893779049	36.0	36.0	36.0	32.0	36.0
16	34.24329415284643	36.0	36.0	36.0	32.0	36.0
17	34.154058854328866	36.0	36.0	36.0	32.0	36.0
18	34.164299916254826	36.0	36.0	36.0	32.0	36.0
19	34.16988537226514	36.0	36.0	36.0	32.0	36.0
20	34.1517684507045	36.0	36.0	36.0	32.0	36.0
21	34.126509967533465	36.0	36.0	36.0	32.0	36.0
22	34.104308940982875	36.0	36.0	36.0	32.0	36.0
23	34.0534321588313	36.0	36.0	36.0	32.0	36.0
24	34.023013056522856	36.0	36.0	36.0	32.0	36.0
25	33.640096192063424	36.0	36.0	36.0	27.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
3	14.0
4	16.0
5	21.0
6	86.0
7	33.0
8	101.0
9	117.0
10	166.0
11	60.0
12	138.0
13	96.0
14	193.0
15	359.0
16	640.0
17	821.0
18	1065.0
19	1465.0
20	2159.0
21	3074.0
22	4770.0
23	7303.0
24	10833.0
25	15581.0
26	21767.0
27	29358.0
28	39956.0
29	53446.0
30	70600.0
31	96221.0
32	136067.0
33	194180.0
34	418931.0
35	935854.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.1968152719828	17.392216598284385	11.43496167771918	25.97600645201364
2	16.59402310089837	19.63287905640775	37.86772596712094	25.905371875572943
3	18.70254403800828	23.95356716300431	28.50811728867354	28.835771510313872
4	12.424917400584919	15.356548845368224	36.05998590016495	36.158547853881906
5	14.576642110929608	36.68115694729179	33.440865524947945	15.30133541683066
6	34.18724101260253	35.62356219975798	16.843721350951608	13.345475436687895
7	30.47755638531547	30.257804752581375	20.764328843110828	18.50031001899233
8	28.126931744379846	33.16267146065306	19.22320401568087	19.48719277928622
9	27.652732018062153	13.948083048340521	18.574748984590823	39.82443594900651
10	16.399450387384935	26.17444477435115	31.031247600091767	26.394857238172154
11	37.340705642271814	20.63278006776537	22.97698061100358	19.04953367895923
12	25.55305367615658	23.197167065918354	28.67964197959047	22.5701372783346
13	29.671264492641964	19.310820177993932	25.229243132000505	25.7886721973636
14	23.605568869979635	20.224797703102855	24.54605804175723	31.62357538516028
15	24.797184031431154	28.36840820587172	21.740502196062202	25.093905566634923
16	25.624183661988365	25.41701113888631	24.37151145441471	24.58729374471062
17	23.85198628361224	26.17540735812784	25.299789166793037	24.67281719146688
18	24.857029771174247	24.332507973504335	26.979236843045136	23.831225412276286
19	25.991089211115835	24.379720739183547	25.0260391313473	24.603150918353318
20	26.213367555172194	24.069642681534837	24.736009597249208	24.98098016604376
21	27.064539533454695	23.86087495957687	23.95529752157898	25.119287985389455
22	26.31847002876769	24.546468619738153	24.474549404097928	24.66051194739623
23	24.085999514646712	24.450454823002616	25.601750011742418	25.861795650608254
24	25.163286453609036	24.657893475097776	25.354729118169978	24.82409095312321
25	24.894542842856847	24.509163104134235	25.344552089622198	25.251741963386724
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	144.0
1	144.0
2	361.0
3	578.0
4	578.0
5	578.0
6	1596.0
7	2614.0
8	2614.0
9	2614.0
10	2795.0
11	2976.0
12	2976.0
13	2976.0
14	3673.0
15	4370.0
16	4370.0
17	4370.0
18	7742.5
19	11115.0
20	11115.0
21	11115.0
22	19205.5
23	27296.0
24	27296.0
25	27296.0
26	43516.0
27	59736.0
28	59736.0
29	59736.0
30	76503.0
31	93270.0
32	93270.0
33	93270.0
34	113639.5
35	134009.0
36	134009.0
37	134009.0
38	151433.0
39	168857.0
40	168857.0
41	168857.0
42	192043.5
43	215230.0
44	215230.0
45	215230.0
46	240308.0
47	265386.0
48	265386.0
49	265386.0
50	269260.5
51	273135.0
52	273135.0
53	273135.0
54	250878.5
55	228622.0
56	228622.0
57	228622.0
58	211792.5
59	194963.0
60	194963.0
61	194963.0
62	173404.0
63	151845.0
64	151845.0
65	151845.0
66	126031.0
67	100217.0
68	100217.0
69	100217.0
70	75429.5
71	50642.0
72	50642.0
73	50642.0
74	39479.0
75	28316.0
76	28316.0
77	28316.0
78	23230.0
79	18144.0
80	18144.0
81	18144.0
82	12399.5
83	6655.0
84	6655.0
85	6655.0
86	5177.5
87	3700.0
88	3700.0
89	3700.0
90	2713.0
91	1726.0
92	1726.0
93	1726.0
94	1217.5
95	709.0
96	709.0
97	709.0
98	972.5
99	1236.0
100	1236.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.041945919097175204
2	0.005671010041109934
3	0.0020532967390225624
4	0.0037643773548746975
5	0.010364259730304363
6	0.013004212680476227
7	0.02224404800607776
8	0.034563828440213135
9	0.048643577507796416
10	0.05583011609437539
11	0.05299461107382042
12	0.06111002199471911
13	0.06179445424105997
14	0.06560771961353044
15	0.05622122023514159
16	0.06360331089210365
17	0.05969226948444163
18	0.0749942189919193
19	0.07098540154906573
20	0.07685196366055877
21	0.07274537018251363
22	0.07484755493913198
23	0.0791497004875602
24	0.07514088304470662
25	0.07494533097432353
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	2045491.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	44.760183751591704
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.23149971402093	33.67375751107395
2	13.836881808593194	12.386827446033756
3	4.519704156618695	6.069083656592567
4	1.9608295806886094	3.5106836934871466
5	1.085035992681062	2.428320520474752
6	0.6865445840680108	1.8437917041926557
7	0.48100037588978284	1.5070765646587954
8	0.3354081730620121	1.2010345166433063
9	0.24402861186630204	0.9830488957003378
>10	1.384450882833844	11.173433827293113
>50	0.1077470837832599	3.348277256257562
>100	0.10101452141790944	9.984970489678963
>500	0.01676161319872954	5.212933587217764
>1k	0.009092901277742168	6.676760330695439
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	4477	0.218871654776286	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTG	2942	0.1438285477667709	No Hit
TATCAACGCAGAGTACTTTTTTTTT	2844	0.13903752204238495	No Hit
GATTAAGAGGGACGGCCGGGGGCAT	2738	0.13385539217723275	No Hit
GAATAGGACCGCGGTTCTATTTTGT	2657	0.12989546275197494	No Hit
GTATCTGATCGTCTTCGAACCTCCG	2560	0.12515332504518473	No Hit
GTCTTGCGCCGGTCCAAGAATTTCA	2544	0.12437111676365234	No Hit
CTATTGGAGCTGGAATTACCGCGGC	2477	0.1210956195847354	No Hit
GCGCAAGACGGACCAGAGCGAAAGC	2451	0.11982453112724524	No Hit
GAACTACGACGGTATCTGATCGTCT	2348	0.11478906531488038	No Hit
GAATAACGCCGCCGCATCGCCAGTC	2335	0.1141535210861353	No Hit
GTCCTATTCCATTATTCCTAGCTGC	2302	0.11254021650547472	No Hit
GGGTAGGCACACGCTGAGCCAGTCA	2276	0.11126912804798456	No Hit
ATCAGATACCGTCGTAGTTCCGACC	2266	0.11078024787202681	No Hit
GTACATGGGGAATAATTGCAATCCC	2230	0.1090202792385789	No Hit
GTCCTAAAGTGTGTATTTCTCATTT	2229	0.10897139122098314	No Hit
TCGTAGTTCCGACCATAAACGATGC	2196	0.10735808664032254	No Hit
CTGTAGGACGTGGAATATGGCAAGA	2047	0.10007377201855201	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	9.777603519155058E-5
2	0.0	0.0	0.0	0.0	9.777603519155058E-5
3	0.0	0.0	0.0	0.0	9.777603519155058E-5
4	0.0	0.0	0.0	0.0	9.777603519155058E-5
5	0.0	0.0	0.0	0.0	9.777603519155058E-5
6	0.0	0.0	0.0	0.0	1.9555207038310116E-4
7	0.0	0.0	0.0	0.0	1.9555207038310116E-4
8	0.0	0.0	0.0	0.0	1.9555207038310116E-4
9	0.0	0.0	0.0	0.0	1.9555207038310116E-4
10	0.0	0.0	0.0	0.0	1.9555207038310116E-4
11	0.0	0.0	0.0	0.0	1.9555207038310116E-4
12	0.0	0.0	0.0	0.0	5.377681935535282E-4
13	0.0	0.0	0.0	0.0	5.866562111493035E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTAAGGT	95	4.9258233E-9	13.9957285	4
CGCCAGT	445	0.0	13.451915	18
ATTGCGA	50	0.001498302	13.301798	10
CGCATCG	460	0.0	13.0119915	13
CGAACGA	185	0.0	12.840201	16
GCGGGTA	60	4.0866522E-4	12.669	18
CCGCATC	510	0.0	12.10948	12
GTGCGAA	55	0.0030670317	12.091952	11
TCGGGAC	55	0.0030777266	12.086334	3
CCTAACC	55	0.0030777266	12.086334	3
GTATAGA	150	1.4551915E-11	12.028779	1
TCGCCAG	525	0.0	11.945057	17
GTCTAAA	160	3.6379788E-12	11.870505	1
CGACCAT	395	0.0	11.786404	10
CGTTATT	210	0.0	11.756591	2
CTCGTAG	180	0.0	11.612681	10
ACTCTAA	475	0.0	11.601569	10
GCGTTAT	230	0.0	11.560841	1
GTATCAA	3065	0.0	11.5568	1
CGTCGTA	370	0.0	11.555616	10
>>END_MODULE