FastQCFastQC Report
Thu 2 Feb 2017
SRR4063993_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4063993_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2045491
Sequences flagged as poor quality0
Sequence length50
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAA28360.13864641790161872No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA27560.1347353764939567No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT26420.1291621424880383No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATACGAATGCC25740.1258377572915256No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCG24710.12080229147916074No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA23980.11723346619466916No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTGATTAATGAAAAC23970.11718457817707338No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT23270.11376241694536912No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCGGCGGCGTTATT22740.11117135201279302No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTGA22210.10858028708021691No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGAC21950.10730919862272675No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA21570.1054514539540873No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTAGGAC10500.021.9987533
GTATCAA35950.020.929931
TAGGACG15850.020.5419254
GTCCTAA8700.019.7249491
GTATAGG2150.019.442661
AGGACGT16200.019.0117345
GTCCTAC15950.018.897331
GACGTGA10300.018.5819247
TAATACC1801.4551915E-1118.3327434
ATAATAC1801.4551915E-1118.3322943
GTAGGAC17000.018.1166213
TCCTACA17000.017.9872152
GGACGTG16700.017.915596
TGTAGGA16750.017.730342
TATGGCG20650.017.68595716
TTTAGGA13100.017.4646592
ATACCGT8200.017.170186
CCTACAG16950.017.1317733
TGGCGAG21200.017.12250918
CGGTCCA8500.017.08263810
Produced by FastQC (version 0.11.5)