##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4063993_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2045491
Sequences flagged as poor quality	0
Sequence length	50
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.343642186643695	32.0	32.0	32.0	32.0	32.0
2	31.42737171661963	32.0	32.0	32.0	32.0	32.0
3	31.503137388529208	32.0	32.0	32.0	32.0	32.0
4	31.578848305859083	32.0	32.0	32.0	32.0	32.0
5	31.533632756145103	32.0	32.0	32.0	32.0	32.0
6	35.13041220909796	36.0	36.0	36.0	36.0	36.0
7	35.14300185138923	36.0	36.0	36.0	36.0	36.0
8	35.096302550341214	36.0	36.0	36.0	36.0	36.0
9	35.20287793981983	36.0	36.0	36.0	36.0	36.0
10	35.055570031840766	36.0	36.0	36.0	36.0	36.0
11	35.19694684552511	36.0	36.0	36.0	36.0	36.0
12	35.09502363980091	36.0	36.0	36.0	36.0	36.0
13	35.14018296829465	36.0	36.0	36.0	36.0	36.0
14	35.08062611861895	36.0	36.0	36.0	36.0	36.0
15	35.05689734151849	36.0	36.0	36.0	36.0	36.0
16	35.0647013357673	36.0	36.0	36.0	36.0	36.0
17	35.027386089696805	36.0	36.0	36.0	36.0	36.0
18	35.0314447729176	36.0	36.0	36.0	36.0	36.0
19	35.023323495434596	36.0	36.0	36.0	36.0	36.0
20	35.0131318104064	36.0	36.0	36.0	36.0	36.0
21	35.010685942886084	36.0	36.0	36.0	36.0	36.0
22	34.99509506519462	36.0	36.0	36.0	36.0	36.0
23	34.94285528511247	36.0	36.0	36.0	36.0	36.0
24	34.90535328681476	36.0	36.0	36.0	32.0	36.0
25	34.87525733430262	36.0	36.0	36.0	32.0	36.0
26	34.81602705658446	36.0	36.0	36.0	32.0	36.0
27	34.80544328965515	36.0	36.0	36.0	32.0	36.0
28	34.77734099050057	36.0	36.0	36.0	32.0	36.0
29	34.74252245548868	36.0	36.0	36.0	32.0	36.0
30	34.72571231063837	36.0	36.0	36.0	32.0	36.0
31	34.72163162780966	36.0	36.0	36.0	32.0	36.0
32	34.68440389129065	36.0	36.0	36.0	32.0	36.0
33	34.668812524719	36.0	36.0	36.0	32.0	36.0
34	34.65900901055052	36.0	36.0	36.0	32.0	36.0
35	34.614973617581306	36.0	36.0	36.0	32.0	36.0
36	34.58481411064629	36.0	36.0	36.0	32.0	36.0
37	34.56011637303708	36.0	36.0	36.0	32.0	36.0
38	34.51310076651523	36.0	36.0	36.0	32.0	36.0
39	34.5030948559539	36.0	36.0	36.0	32.0	36.0
40	34.48273299662526	36.0	36.0	36.0	32.0	36.0
41	34.47334747500722	36.0	36.0	36.0	32.0	36.0
42	34.399704031941475	36.0	36.0	36.0	32.0	36.0
43	34.40245104965018	36.0	36.0	36.0	32.0	36.0
44	34.31875183024516	36.0	36.0	36.0	32.0	36.0
45	34.299808701187146	36.0	36.0	36.0	32.0	36.0
46	34.2622035491723	36.0	36.0	36.0	32.0	36.0
47	34.23648796303675	36.0	36.0	36.0	32.0	36.0
48	34.193425441617684	36.0	36.0	36.0	32.0	36.0
49	34.16866659398648	36.0	36.0	36.0	32.0	36.0
50	33.58954353746851	36.0	36.0	36.0	27.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	2.0
18	1.0
19	5.0
20	16.0
21	55.0
22	241.0
23	659.0
24	1708.0
25	3786.0
26	7728.0
27	14031.0
28	23066.0
29	35781.0
30	51215.0
31	72833.0
32	104936.0
33	170107.0
34	356208.0
35	1203113.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.27246289370661	18.270959426932247	12.036046043731082	26.420531635630063
2	15.773145022471466	20.36580113352201	37.42734601069772	26.433707833308805
3	17.945501353715162	24.419672233732683	28.88175012050949	28.753076292042657
4	12.134641511500172	15.934853783272573	36.15655116546589	35.77395353976136
5	14.235457403625828	37.06420610014906	33.201270501801275	15.499065994423834
6	34.34386742319429	35.913616617273355	16.55425654343271	13.188259416099646
7	30.353396812794582	30.421497821305493	20.828935448750446	18.39616991714948
8	28.085530564544158	33.333659253450634	19.151392012969012	19.429418169036186
9	27.611013605674977	13.75287831158609	18.489784743848602	40.146323338890326
10	16.249950500543395	26.34544411890234	31.057401280972602	26.34720409958166
11	37.50053165719135	20.58464202482436	22.95038208430152	18.964444233682766
12	25.433881792511126	23.235922185806025	28.731041198970615	22.599154822712233
13	29.698027754773193	19.313258560557248	25.336570379851047	25.652143304818516
14	23.619276788010282	20.12925045685964	24.59620843163923	31.65526432349085
15	24.925980393115495	28.403337433582802	21.66662429524925	25.004057878052453
16	25.67232549896723	25.40691037534069	24.315232403231523	24.60553172246055
17	23.867081302239903	26.31881538466803	25.16852921865704	24.645574094435027
18	25.028734016626863	24.27580744908909	26.975167258620814	23.72029127566323
19	26.01852173245615	24.342932179053516	25.057040150301468	24.58150593818886
20	26.301969515194003	23.965889635576623	24.801293389613576	24.930847459615794
21	27.157000446347602	23.837650715647246	23.999421165871667	25.005927672133488
22	26.38239560320295	24.548995374700134	24.51765812478092	24.550950897315996
23	24.134114373266296	24.279997144921627	25.678643329334268	25.90724515247781
24	25.27106080620607	24.623585052325076	25.31642908615182	24.788925055317033
25	24.98473446276309	24.477708404527693	25.38713842432278	25.15041870838644
26	24.35437614220494	25.653495232684687	25.817327389548968	24.174801235561404
27	25.310325102488324	24.782788317920364	24.830457304477708	25.07642927511361
28	24.291026497221598	24.77444752232058	25.588949370838876	25.34557660961894
29	24.67572912363385	24.429522326546678	25.33470875388365	25.56003979593583
30	24.162362345233877	24.831856460148867	25.977975237420097	25.02780595719716
31	25.72871426832291	24.33374451010561	24.49684625948921	25.440694962082265
32	24.88182185741964	24.906853281145423	24.462203283516562	25.74912157791837
33	24.115409624476268	24.67456402383874	25.57443250986355	25.635593841821446
34	25.31997942744949	24.578917829087473	25.617567628348663	24.48353511511437
35	25.80692788799297	24.457880273368655	25.57089100014031	24.164300838498065
36	24.352451682613562	25.09707479966558	25.29298450616771	25.257489011553147
37	25.620731295926003	24.822651693303545	24.53072978033744	25.02588723043302
38	24.726780431352744	24.93867746718994	25.103100570909483	25.231441530547837
39	25.92311565896324	24.193535770334552	24.49914687030962	25.384201700392587
40	26.181512047954214	24.453286830141614	25.21659037542332	24.148610746480852
41	24.947945058141666	24.852317477700343	25.83729133411719	24.362446130040798
42	26.06984754003388	25.556367654140523	25.0645455412121	23.309239264613495
43	24.852026167180732	24.5314141590437	25.479121352965073	25.137438320810496
44	24.471538514939873	25.250774151220305	24.82314543483001	25.454541899009808
45	24.694030602317515	25.39704888763734	24.838692014435832	25.070228495609314
46	24.582089326038524	24.700399369730132	25.138879858106165	25.57863144612518
47	25.42501566889307	24.39341094935403	25.428682336537513	24.75289104521539
48	25.660045838010348	25.333860679043973	24.195537051739848	24.81055643120583
49	24.96951824651941	25.34644578354484	24.476236943200785	25.207799026734968
50	24.832715463021405	25.98751287603271	24.226399154814036	24.953372506131853
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	35.0
1	37.0
2	39.0
3	109.5
4	180.0
5	218.5
6	257.0
7	257.5
8	258.0
9	333.5
10	409.0
11	637.5
12	866.0
13	1648.0
14	2430.0
15	3262.0
16	4094.0
17	4411.0
18	4728.0
19	4717.5
20	4707.0
21	5115.5
22	5524.0
23	6363.0
24	7202.0
25	8766.5
26	10331.0
27	13635.0
28	16939.0
29	20803.0
30	24667.0
31	29464.0
32	34261.0
33	42239.5
34	50218.0
35	58944.0
36	67670.0
37	76874.0
38	86078.0
39	89575.0
40	93072.0
41	98186.5
42	103301.0
43	103993.5
44	104686.0
45	116051.5
46	127417.0
47	136556.5
48	145696.0
49	151773.0
50	157850.0
51	150660.5
52	143471.0
53	142846.0
54	142221.0
55	144450.5
56	146680.0
57	143747.5
58	140815.0
59	128321.0
60	115827.0
61	101941.0
62	88055.0
63	76459.0
64	64863.0
65	55304.0
66	45745.0
67	39457.0
68	33169.0
69	30593.5
70	28018.0
71	21478.0
72	14938.0
73	12868.0
74	10798.0
75	8330.0
76	5862.0
77	5216.0
78	4570.0
79	3850.5
80	3131.0
81	2519.5
82	1908.0
83	1584.5
84	1261.0
85	933.5
86	606.0
87	437.5
88	269.0
89	202.0
90	135.0
91	113.5
92	92.0
93	68.5
94	45.0
95	49.0
96	53.0
97	38.5
98	24.0
99	22.0
100	20.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.010657587835879013
2	3.911041407662023E-4
3	4.3999215836197766E-4
4	0.0
5	0.0
6	2.444400879788764E-4
7	0.0
8	0.0
9	0.0010266483695112812
10	6.844322463408541E-4
11	0.0
12	1.4666405278732588E-4
13	2.444400879788764E-4
14	2.444400879788764E-4
15	0.004253257530832451
16	7.822082815324047E-4
17	0.0
18	2.9332810557465176E-4
19	9.777603519155058E-5
20	6.355442287450788E-4
21	0.0
22	9.777603519155058E-5
23	6.355442287450788E-4
24	4.3999215836197766E-4
25	0.0016621925982563599
26	0.0051332418475564055
27	0.0066487703930254395
28	0.003373273214108495
29	0.0027377289853634163
30	0.003226609161321169
31	0.0069420984986000925
32	0.0030310570909380685
33	0.003959929425257799
34	0.002297736827001439
35	0.002346624844597214
36	0.007870970832919823
37	0.003959929425257799
38	0.007724306780132496
39	0.003959929425257799
40	0.0019555207038310114
41	0.0027377289853634163
42	0.0017599686334479107
43	0.0013688644926817082
44	0.0012222004398943823
45	0.0012222004398943823
46	8.799843167239553E-4
47	0.001808856651043686
48	0.0013199764750859329
49	2.444400879788764E-4
50	0.001173312422298607
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	2045491.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	49.16233721353073
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.56073022228746	37.6390443650225
2	13.43142842705753	13.206408271808098
3	4.277300663060501	6.30846292683117
4	1.8308810637441018	3.6004156901462157
5	1.004984043895461	2.4703682230103214
6	0.6032420978296191	1.7794074860938456
7	0.40738889330542516	1.4019733104809884
8	0.29979478601232046	1.1790889891836789
9	0.22081772029789504	0.9770323705207404
>10	1.1782586318913988	10.353339860510905
>50	0.08359394573253265	2.844177381652226
>100	0.08192722101201719	8.741082485938236
>500	0.01313494483408144	4.4749114024347705
>1k	0.006517339039811402	5.024287236366271
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAA	2836	0.13864641790161872	No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA	2756	0.1347353764939567	No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT	2642	0.1291621424880383	No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATACGAATGCC	2574	0.1258377572915256	No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCG	2471	0.12080229147916074	No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA	2398	0.11723346619466916	No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTGATTAATGAAAAC	2397	0.11718457817707338	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT	2327	0.11376241694536912	No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCGGCGGCGTTATT	2274	0.11117135201279302	No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTGA	2221	0.10858028708021691	No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGAC	2195	0.10730919862272675	No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA	2157	0.1054514539540873	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	9.777603519155058E-5
2	0.0	0.0	0.0	0.0	9.777603519155058E-5
3	0.0	0.0	0.0	0.0	1.4666405278732588E-4
4	0.0	0.0	0.0	0.0	1.4666405278732588E-4
5	0.0	0.0	0.0	0.0	1.4666405278732588E-4
6	0.0	0.0	0.0	0.0	1.9555207038310116E-4
7	0.0	0.0	0.0	0.0	2.444400879788765E-4
8	0.0	0.0	0.0	0.0	2.444400879788765E-4
9	0.0	0.0	0.0	0.0	2.444400879788765E-4
10	0.0	0.0	0.0	0.0	2.444400879788765E-4
11	0.0	0.0	0.0	0.0	2.444400879788765E-4
12	0.0	0.0	0.0	0.0	4.88880175957753E-4
13	0.0	0.0	0.0	0.0	6.355442287450788E-4
14	0.0	0.0	0.0	0.0	6.355442287450788E-4
15	0.0	0.0	0.0	0.0	7.822082815324047E-4
16	0.0	0.0	0.0	0.0	7.822082815324047E-4
17	0.0	0.0	0.0	0.0	7.822082815324047E-4
18	0.0	0.0	0.0	0.0	8.310962991281799E-4
19	0.0	0.0	0.0	0.0	8.310962991281799E-4
20	0.0	0.0	0.0	9.777603519155058E-5	9.77760351915506E-4
21	0.0	0.0	0.0	1.9555207038310116E-4	9.77760351915506E-4
22	0.0	0.0	0.0	6.844322463408541E-4	0.001173312422298607
23	0.0	0.0	0.0	8.310962991281799E-4	0.0012222004398943823
24	0.0	0.0	0.0	0.0019066326862352365	0.0012222004398943823
25	0.0	0.0	0.0	0.0022977368270014386	0.0012222004398943823
26	0.0	0.0	0.0	0.0026888409677676413	0.0012222004398943823
27	0.0	0.0	0.0	0.0034221612317042703	0.0013199764750859329
28	0.0	0.0	0.0	0.005671010041109934	0.0013199764750859329
29	0.0	0.0	0.0	0.010266483695112811	0.0013688644926817082
30	0.0	0.0	0.0	0.01847967065120306	0.0013688644926817082
31	0.0	0.0	0.0	0.04008817442853574	0.0014177525102774834
32	0.0	0.0	0.0	0.060816693889144464	0.0014177525102774834
33	0.0	0.0	0.0	0.08218075757849827	0.0014177525102774834
34	0.0	0.0	0.0	0.10452258161976757	0.001515528545469034
35	0.0	0.0	0.0	0.133806504159637	0.001515528545469034
36	0.0	0.0	0.0	0.17462799885210933	0.001515528545469034
37	0.0	0.0	0.0	0.2313380992632087	0.001515528545469034
38	0.0	0.0	0.0	0.30662564636070266	0.0015644165630648093
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTAGGAC	1050	0.0	21.998753	3
GTATCAA	3595	0.0	20.92993	1
TAGGACG	1585	0.0	20.541925	4
GTCCTAA	870	0.0	19.724949	1
GTATAGG	215	0.0	19.44266	1
AGGACGT	1620	0.0	19.011734	5
GTCCTAC	1595	0.0	18.89733	1
GACGTGA	1030	0.0	18.581924	7
TAATACC	180	1.4551915E-11	18.332743	4
ATAATAC	180	1.4551915E-11	18.332294	3
GTAGGAC	1700	0.0	18.116621	3
TCCTACA	1700	0.0	17.987215	2
GGACGTG	1670	0.0	17.91559	6
TGTAGGA	1675	0.0	17.73034	2
TATGGCG	2065	0.0	17.685957	16
TTTAGGA	1310	0.0	17.464659	2
ATACCGT	820	0.0	17.17018	6
CCTACAG	1695	0.0	17.131773	3
TGGCGAG	2120	0.0	17.122509	18
CGGTCCA	850	0.0	17.082638	10
>>END_MODULE