FastQCFastQC Report
Thu 2 Feb 2017
SRR4063992_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4063992_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1416412
Sequences flagged as poor quality0
Sequence length25
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT29940.21137917498580922No Hit
TATCAACGCAGAGTACTTTTTTTTT20340.14360228521080026No Hit
GTTCAAAGCAGGCCCGAGCCGCCTG14410.10173593558936242No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATTAT1051.378794E-913.5660531
CTTATAC1051.380613E-913.5646163
AACCGCG1001.0064468E-813.3003717
GGTATAT655.472115E-513.1486371
CGCGCTA801.995244E-613.06471116
CGCCAGT2050.012.51475518
CGCATCG2000.012.35209113
GTATGAA1251.4188117E-912.1551861
TATACCG550.00307488612.0874025
CGTTATT951.0467593E-611.9931292
ACGCGCG802.8609753E-511.8770113
CGTGAAA1603.6379788E-1211.8765919
TTATACT1201.0062649E-811.8707164
TAGTATA658.046962E-411.688094
GTATTAC1157.109338E-811.5606381
AGAACCG1657.2759576E-1211.5118115
TCGCCAG2450.011.24719917
CGGTCCA2450.011.24719910
GCGTTAT1104.991871E-711.2228271
TTACCGC2550.011.17836316
Produced by FastQC (version 0.11.5)