##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4063992_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1416412
Sequences flagged as poor quality	0
Sequence length	25
%GC	51
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.228267622697352	32.0	32.0	32.0	32.0	32.0
2	30.770756672493597	32.0	32.0	32.0	32.0	32.0
3	30.78550803014942	32.0	32.0	32.0	32.0	32.0
4	30.823822447141087	32.0	32.0	32.0	32.0	32.0
5	30.707763701521873	32.0	32.0	32.0	32.0	32.0
6	34.36754418912012	36.0	36.0	36.0	32.0	36.0
7	34.306194101716166	36.0	36.0	36.0	32.0	36.0
8	34.26168374738423	36.0	36.0	36.0	32.0	36.0
9	34.42776466169448	36.0	36.0	36.0	32.0	36.0
10	34.075122916213644	36.0	36.0	36.0	32.0	36.0
11	34.41151585838019	36.0	36.0	36.0	32.0	36.0
12	34.19383908071945	36.0	36.0	36.0	32.0	36.0
13	34.28422168126223	36.0	36.0	36.0	32.0	36.0
14	34.16458135062397	36.0	36.0	36.0	32.0	36.0
15	34.09581392984527	36.0	36.0	36.0	32.0	36.0
16	34.10417237357492	36.0	36.0	36.0	32.0	36.0
17	34.0212628811391	36.0	36.0	36.0	32.0	36.0
18	34.015422066460886	36.0	36.0	36.0	32.0	36.0
19	34.03292544824529	36.0	36.0	36.0	32.0	36.0
20	34.0114627664832	36.0	36.0	36.0	32.0	36.0
21	34.002437143994825	36.0	36.0	36.0	32.0	36.0
22	33.97566103647809	36.0	36.0	36.0	32.0	36.0
23	33.91888306509688	36.0	36.0	36.0	32.0	36.0
24	33.901439694100304	36.0	36.0	36.0	32.0	36.0
25	33.48584663219459	36.0	36.0	36.0	27.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	1.0
3	2.0
4	15.0
5	15.0
6	54.0
7	26.0
8	57.0
9	90.0
10	155.0
11	41.0
12	91.0
13	78.0
14	123.0
15	249.0
16	362.0
17	520.0
18	667.0
19	984.0
20	1427.0
21	2215.0
22	3507.0
23	5581.0
24	8118.0
25	12186.0
26	17293.0
27	22433.0
28	30362.0
29	40592.0
30	53710.0
31	72479.0
32	101306.0
33	142228.0
34	298317.0
35	601128.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.58221301830315	16.913566818427743	11.207835448241376	26.296384715027738
2	17.0117508672116	19.530177127925096	37.13882657956777	26.319245425295534
3	19.0967716427584	23.507504310265702	28.29588826774622	29.09983577922967
4	12.917558330903395	15.224035714109204	35.50950006319019	36.34890589179721
5	14.87198858982687	36.259779139718134	33.44084503064366	15.427387239811335
6	34.57351335855494	35.00528862923532	16.74235973128962	13.678838280920122
7	30.478887362782864	30.094379261278377	20.69776392120585	18.728969454732912
8	28.376428511395957	32.730205657614924	19.193557158465623	19.699808672523496
9	27.689750700347382	13.905586250446767	18.190313662389475	40.21434938681638
10	16.178579451452887	26.433638458484566	31.254017748026623	26.13376434203592
11	37.61518698702277	20.724721732256874	22.31595335796778	19.344137922752576
12	25.164641702072345	23.214585961972396	28.526850620770922	23.093921715184337
13	29.986994059372368	19.196443394486923	24.937566539762784	25.878996006377925
14	23.859026404995287	19.30318620559073	24.768089053803337	32.06969833561064
15	25.43518512631513	26.993734577516676	21.8456893342291	25.725390961939095
16	25.955363387804415	25.318807712144014	23.530799827614224	25.195029072437354
17	24.303312071402786	25.599091216719806	24.917150529240512	25.1804461826369
18	25.086661174239484	24.574466431424973	25.949740051805932	24.389132342529614
19	26.041239793914077	24.31125700542346	25.007983992448413	24.639519208214043
20	26.26362973472537	23.904251290196765	24.636823035311544	25.19529593976632
21	27.114061050461345	23.725077208633234	23.9740666604961	25.18679508040932
22	26.418240644632036	23.954730483580015	24.429485245435988	25.197543626351965
23	24.818722045179936	23.935049426594972	25.048366696579354	26.197861831645742
24	25.237050205184442	24.526323524424296	24.954003074940367	25.2826231954509
25	25.206902667247945	24.071296189655893	25.015491645110256	25.706309497985906
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	95.0
1	95.0
2	246.5
3	398.0
4	398.0
5	398.0
6	1066.0
7	1734.0
8	1734.0
9	1734.0
10	1894.0
11	2054.0
12	2054.0
13	2054.0
14	2524.0
15	2994.0
16	2994.0
17	2994.0
18	5206.0
19	7418.0
20	7418.0
21	7418.0
22	12309.0
23	17200.0
24	17200.0
25	17200.0
26	26383.0
27	35566.0
28	35566.0
29	35566.0
30	46021.0
31	56476.0
32	56476.0
33	56476.0
34	70501.5
35	84527.0
36	84527.0
37	84527.0
38	98092.5
39	111658.0
40	111658.0
41	111658.0
42	129299.0
43	146940.0
44	146940.0
45	146940.0
46	165247.5
47	183555.0
48	183555.0
49	183555.0
50	188484.5
51	193414.0
52	193414.0
53	193414.0
54	181541.0
55	169668.0
56	169668.0
57	169668.0
58	156397.0
59	143126.0
60	143126.0
61	143126.0
62	126696.5
63	110267.0
64	110267.0
65	110267.0
66	90899.5
67	71532.0
68	71532.0
69	71532.0
70	54238.0
71	36944.0
72	36944.0
73	36944.0
74	28319.0
75	19694.0
76	19694.0
77	19694.0
78	15750.5
79	11807.0
80	11807.0
81	11807.0
82	8240.0
83	4673.0
84	4673.0
85	4673.0
86	3575.0
87	2477.0
88	2477.0
89	2477.0
90	1743.0
91	1009.0
92	1009.0
93	1009.0
94	683.5
95	358.0
96	358.0
97	358.0
98	593.0
99	828.0
100	828.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.04165454684089093
2	0.006424684343256058
3	0.0018356240980731593
4	0.0037418491229952865
5	0.009319322344063734
6	0.011719753856928633
7	0.022380493811122754
8	0.03635947732721835
9	0.048432235818391824
10	0.05775155816245556
11	0.05499812201534581
12	0.06452924713995646
13	0.06389383879831574
14	0.07045972499526974
15	0.06106980172435704
16	0.06933011016568626
17	0.06304662767612813
18	0.07977904733933347
19	0.07624900099688509
20	0.07935544177823967
21	0.07702561119222373
22	0.08154407051055766
23	0.08345029553547978
24	0.07766101953386444
25	0.07999085011988037
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	1416412.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	54.662122704934035
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.2483987240442	43.31885695223242
2	12.180368929480686	13.316096420292789
3	3.605511698221956	5.9125476858685095
4	1.5137265636918429	3.3097402866496672
5	0.8518732539227516	2.328260016748844
6	0.5299165929247512	1.73798194955
7	0.35187235975231135	1.3463863073678877
8	0.26168861254936093	1.1443564039725689
9	0.20188269906703735	0.9931803181565101
>10	1.0952210469896573	10.712234924406312
>50	0.07563256851292131	2.85691753679057
>100	0.07312638713647726	8.307145267757548
>500	0.008442610131220498	3.0240439657264875
>1k	0.002337953574799522	1.6922519644798635
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	2994	0.21137917498580922	No Hit
TATCAACGCAGAGTACTTTTTTTTT	2034	0.14360228521080026	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTG	1441	0.10173593558936242	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	7.060092684896767E-5
2	0.0	0.0	0.0	0.0	7.060092684896767E-5
3	0.0	0.0	0.0	0.0	7.060092684896767E-5
4	0.0	0.0	0.0	0.0	7.060092684896767E-5
5	0.0	0.0	0.0	0.0	7.060092684896767E-5
6	0.0	0.0	0.0	0.0	7.060092684896767E-5
7	0.0	0.0	0.0	0.0	7.060092684896767E-5
8	0.0	0.0	0.0	0.0	7.060092684896767E-5
9	0.0	0.0	0.0	0.0	7.060092684896767E-5
10	0.0	0.0	0.0	0.0	7.060092684896767E-5
11	0.0	0.0	0.0	0.0	7.060092684896767E-5
12	0.0	0.0	0.0	0.0	7.060092684896767E-5
13	0.0	0.0	0.0	0.0	7.060092684896767E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATTAT	105	1.378794E-9	13.566053	1
CTTATAC	105	1.380613E-9	13.564616	3
AACCGCG	100	1.0064468E-8	13.300371	7
GGTATAT	65	5.472115E-5	13.148637	1
CGCGCTA	80	1.995244E-6	13.064711	16
CGCCAGT	205	0.0	12.514755	18
CGCATCG	200	0.0	12.352091	13
GTATGAA	125	1.4188117E-9	12.155186	1
TATACCG	55	0.003074886	12.087402	5
CGTTATT	95	1.0467593E-6	11.993129	2
ACGCGCG	80	2.8609753E-5	11.87701	13
CGTGAAA	160	3.6379788E-12	11.876591	9
TTATACT	120	1.0062649E-8	11.870716	4
TAGTATA	65	8.046962E-4	11.68809	4
GTATTAC	115	7.109338E-8	11.560638	1
AGAACCG	165	7.2759576E-12	11.511811	5
TCGCCAG	245	0.0	11.247199	17
CGGTCCA	245	0.0	11.247199	10
GCGTTAT	110	4.991871E-7	11.222827	1
TTACCGC	255	0.0	11.178363	16
>>END_MODULE