FastQCFastQC Report
Thu 2 Feb 2017
SRR4063992_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4063992_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1416412
Sequences flagged as poor quality0
Sequence length50
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA14290.10088872446717481No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACGCT651.8143863E-523.6911093
GTATCAA22250.023.4325241
TGTAGGA5700.022.7699762
GTAGGAC5150.021.7853093
TAGGACG4350.021.7460274
TCGATCG654.933246E-420.30738341
ATACCGT3400.020.057816
AGGACGT5000.019.7995
CTGTAGG6950.019.6249071
ATCGTTT3450.019.13014429
GGTATCA11300.018.8841
ACCGTAC708.1270264E-418.856198
CAACGTA600.007413367318.33305420
ACGATTA600.00741487718.33240540
GGTATAT1203.164605E-718.3324051
CGTCGTA3700.017.83756610
ACCGTCG3600.017.7213278
GTCCTAC4350.017.7002541
TACCGTC3750.017.599117
CCTTATA1254.8583024E-717.598492
Produced by FastQC (version 0.11.5)