##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4063992_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1416412
Sequences flagged as poor quality	0
Sequence length	50
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.30865595603539	32.0	32.0	32.0	32.0	32.0
2	31.390130131628368	32.0	32.0	32.0	32.0	32.0
3	31.47345758155113	32.0	32.0	32.0	32.0	32.0
4	31.552732538272764	32.0	32.0	32.0	32.0	32.0
5	31.499325761148594	32.0	32.0	32.0	32.0	32.0
6	35.09666043495819	36.0	36.0	36.0	36.0	36.0
7	35.104725884841415	36.0	36.0	36.0	36.0	36.0
8	35.053483026125164	36.0	36.0	36.0	36.0	36.0
9	35.168570302990936	36.0	36.0	36.0	36.0	36.0
10	35.011956266961874	36.0	36.0	36.0	36.0	36.0
11	35.15846801636812	36.0	36.0	36.0	36.0	36.0
12	35.06341375249574	36.0	36.0	36.0	36.0	36.0
13	35.10942720056029	36.0	36.0	36.0	36.0	36.0
14	35.05011606792374	36.0	36.0	36.0	36.0	36.0
15	35.02456700451564	36.0	36.0	36.0	36.0	36.0
16	35.037897871523256	36.0	36.0	36.0	36.0	36.0
17	34.998029528131646	36.0	36.0	36.0	36.0	36.0
18	34.997025582951856	36.0	36.0	36.0	36.0	36.0
19	34.991752399725506	36.0	36.0	36.0	36.0	36.0
20	34.98650675086063	36.0	36.0	36.0	36.0	36.0
21	34.98030657746475	36.0	36.0	36.0	36.0	36.0
22	34.96878309418446	36.0	36.0	36.0	36.0	36.0
23	34.91670573251286	36.0	36.0	36.0	32.0	36.0
24	34.87743608498092	36.0	36.0	36.0	32.0	36.0
25	34.85528716220986	36.0	36.0	36.0	32.0	36.0
26	34.796585315572024	36.0	36.0	36.0	32.0	36.0
27	34.7933616772521	36.0	36.0	36.0	32.0	36.0
28	34.771495158188436	36.0	36.0	36.0	32.0	36.0
29	34.73157245208315	36.0	36.0	36.0	32.0	36.0
30	34.71377113438745	36.0	36.0	36.0	32.0	36.0
31	34.7163445381711	36.0	36.0	36.0	32.0	36.0
32	34.66777604256389	36.0	36.0	36.0	32.0	36.0
33	34.65295337797195	36.0	36.0	36.0	32.0	36.0
34	34.63840182093911	36.0	36.0	36.0	32.0	36.0
35	34.60532175666402	36.0	36.0	36.0	32.0	36.0
36	34.57707926789663	36.0	36.0	36.0	32.0	36.0
37	34.5735711078415	36.0	36.0	36.0	32.0	36.0
38	34.52613505110095	36.0	36.0	36.0	32.0	36.0
39	34.52083574553167	36.0	36.0	36.0	32.0	36.0
40	34.50552099247959	36.0	36.0	36.0	32.0	36.0
41	34.48914157745063	36.0	36.0	36.0	32.0	36.0
42	34.42439276142817	36.0	36.0	36.0	32.0	36.0
43	34.41259817058879	36.0	36.0	36.0	32.0	36.0
44	34.34354411004708	36.0	36.0	36.0	32.0	36.0
45	34.32440490478759	36.0	36.0	36.0	32.0	36.0
46	34.28942355755246	36.0	36.0	36.0	32.0	36.0
47	34.27556883166762	36.0	36.0	36.0	32.0	36.0
48	34.23338830792171	36.0	36.0	36.0	32.0	36.0
49	34.212983934053085	36.0	36.0	36.0	32.0	36.0
50	33.612929006532	36.0	36.0	36.0	27.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	3.0
18	0.0
19	4.0
20	10.0
21	39.0
22	154.0
23	451.0
24	1157.0
25	2582.0
26	5399.0
27	9820.0
28	16160.0
29	24595.0
30	36026.0
31	51092.0
32	74116.0
33	119385.0
34	249426.0
35	825993.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.71296636258367	17.803259242522664	11.713785409664755	26.76998898522891
2	16.201109430013915	20.196935476696957	36.68948738459323	26.912467708695903
3	18.31264243817442	24.031702819252136	28.651429959248915	29.004224783324535
4	12.459863373086362	15.83508188295496	35.59232765607747	36.11272708788121
5	14.528470529761114	36.66412032657165	33.224019564928845	15.583389578738391
6	34.59410382170687	35.32418955259392	16.541126186009834	13.540580439689384
7	30.467286684443994	30.17062138037618	20.73402423449126	18.62806770068857
8	28.304405780239083	32.960254502221105	19.08484254581294	19.650497171726872
9	27.77021946561517	13.732186289841463	18.154328418273153	40.34326582627021
10	16.079426715617057	26.57427280429257	31.360067777463996	25.986232702626378
11	37.789428499617344	20.620624507558535	22.320341821447432	19.26960517137669
12	25.006742398034465	23.242069739694017	28.61819670858014	23.132991153691375
13	29.980401044336286	19.118795370240935	25.019803699797095	25.880999885625688
14	23.867504184884712	19.253999733127554	24.799016102010228	32.079479979977506
15	25.396956519590297	27.139971433392713	21.843922959122075	25.619149087894915
16	26.01699659912694	25.273916073202436	23.57573879148631	25.133348536184315
17	24.46463317170428	25.592059372555443	24.81064831419107	25.13265914154921
18	25.201460029228823	24.563932759582325	25.866592300252044	24.368014910936804
19	26.16258546242195	24.31135855951517	24.92339799436887	24.60265798369401
20	26.34620367422122	23.862528408108112	24.602498289688924	25.18876962798175
21	27.17126090431315	23.763001160679238	24.051052942223023	25.014684992784588
22	26.42610543557303	23.957540542639492	24.493331733043398	25.123022288744078
23	24.852019621520412	23.773584372820185	25.111903101092626	26.262492904566777
24	25.31659332381159	24.366301493850283	24.976860464541616	25.340244717796512
25	25.300253815170308	24.030330736346404	25.02702301987101	25.642392428612276
26	25.009160929465434	24.743617532783418	25.298356822262434	24.948864715488714
27	25.581727025162692	24.442525574666586	24.582816450534935	25.39293094963579
28	25.052952639159535	24.3158519020588	25.203408737891497	25.42778672089017
29	24.994281179573786	24.42917699819822	25.05471710901625	25.521824713211743
30	24.840947361360676	24.579431004232696	25.32288868256899	25.25673295183764
31	25.612839498502098	24.30112750182339	24.417273816525537	25.66875918314897
32	25.352838594435067	24.527206873910064	24.31793952145273	25.802015010202133
33	24.889257598757368	24.20305715395206	24.961414904508068	25.94627034278251
34	25.654259153485697	24.27171373642395	25.081175650764308	24.99285145932604
35	26.113374330153842	24.16642543968031	24.944400121437198	24.775800108728653
36	25.027589863875882	24.677165054110027	24.690509863508726	25.604735218505365
37	25.764355854036697	24.438982854593057	24.501043522956092	25.295617768414157
38	25.0951593190469	24.471072343676415	24.922880529601944	25.510887807674738
39	25.83289852995272	24.15675797419986	24.419405104250437	25.590938391596985
40	26.05106568048507	24.380600576684657	24.84262608975608	24.725707653074192
41	25.14203692995481	24.64166873182397	25.137659502512435	25.078634835708797
42	26.108754175236005	24.836291211370902	24.62709697279063	24.42785764060246
43	25.25326692982376	24.063200062694552	25.072596479361987	25.610936528119705
44	25.03041175718886	24.64923223992211	24.51035835393143	25.8099976489576
45	25.18012328516631	24.854365778940984	24.648631627848523	25.316879308044175
46	25.091128854046275	24.301945564850634	24.736427042906634	25.870498538196458
47	25.4245150139687	24.3092077626006	25.016573930319595	25.249703293111104
48	25.9217133206814	24.798147959875287	24.179177398219693	25.100961321223625
49	25.202184403472174	24.848613214440785	24.397894669956685	25.551307712130356
50	25.216642308968574	25.268322990136898	24.30262851333319	25.21240618756134
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	13.0
1	17.0
2	21.0
3	75.5
4	130.0
5	174.0
6	218.0
7	190.5
8	163.0
9	241.5
10	320.0
11	435.0
12	550.0
13	1123.0
14	1696.0
15	2185.5
16	2675.0
17	2867.5
18	3060.0
19	2994.5
20	2929.0
21	3298.5
22	3668.0
23	4257.5
24	4847.0
25	5796.0
26	6745.0
27	8511.0
28	10277.0
29	12469.5
30	14662.0
31	17050.5
32	19439.0
33	23499.5
34	27560.0
35	32574.0
36	37588.0
37	43730.0
38	49872.0
39	54150.0
40	58428.0
41	63745.5
42	69063.0
43	71892.0
44	74721.0
45	82407.5
46	90094.0
47	96759.0
48	103424.0
49	107440.5
50	111457.0
51	109088.0
52	106719.0
53	106224.5
54	105730.0
55	105731.5
56	105733.0
57	103527.0
58	101321.0
59	92588.0
60	83855.0
61	73422.0
62	62989.0
63	55239.0
64	47489.0
65	40465.5
66	33442.0
67	28739.5
68	24037.0
69	21668.0
70	19299.0
71	15182.0
72	11065.0
73	9561.0
74	8057.0
75	6317.0
76	4577.0
77	3965.0
78	3353.0
79	2791.5
80	2230.0
81	1766.0
82	1302.0
83	1070.0
84	838.0
85	605.5
86	373.0
87	261.0
88	149.0
89	118.5
90	88.0
91	68.5
92	49.0
93	42.5
94	36.0
95	33.0
96	30.0
97	23.0
98	16.0
99	15.5
100	15.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.009319322344063734
2	6.354083416407091E-4
3	5.648074147917414E-4
4	0.0
5	0.0
6	2.1180278054690305E-4
7	7.060092684896767E-5
8	0.0
9	0.0012002157564324505
10	8.472111221876122E-4
11	0.0
12	1.4120185369793535E-4
13	7.060092684896767E-4
14	3.5300463424483837E-4
15	0.00515386765997464
16	7.766101953386444E-4
17	0.0
18	1.4120185369793535E-4
19	0.0
20	2.1180278054690305E-4
21	0.0
22	1.4120185369793535E-4
23	5.648074147917414E-4
24	3.5300463424483837E-4
25	0.0019062250249221272
26	0.005012665806276705
27	0.00487146395257877
28	0.0036712481961463186
29	0.0025416333665628362
30	0.004024252830391158
31	0.00670708805065193
32	0.0029652389276566423
33	0.004377257464635995
34	0.002753436147109739
35	0.0029652389276566423
36	0.009248721417214766
37	0.00395365190354219
38	0.007271895465443669
39	0.004447858391484963
40	0.002824037073958707
41	0.003883050976693222
42	0.0017650231712241918
43	0.0014826194638283211
44	0.0013414176101303858
45	0.0014826194638283211
46	7.766101953386444E-4
47	0.0021886287323179977
48	0.001976825951771095
49	4.942064879427737E-4
50	0.0015532203906772888
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	1416412.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	57.95028062457499
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.03313720452128	46.379427602671214
2	12.035298931176417	13.948979009246418
3	3.3892332694890834	5.8922105720711455
4	1.4396650696553928	3.337159791677133
5	0.7711528608597609	2.2344262345633488
6	0.4768109228148348	1.6578796069189345
7	0.3257043765461468	1.3212262015051017
8	0.24641890419440773	1.1424035719412953
9	0.18062314203015895	0.9420445591146159
>10	0.9706256056383311	9.943126842460645
>50	0.06440255460718541	2.6028365043991997
>100	0.058959690461881056	7.074597374616013
>500	0.006619127265722836	2.5817084016404923
>1k	0.0013483407393139108	0.9419737271744275
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA	1429	0.10088872446717481	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	7.060092684896767E-5
2	0.0	0.0	0.0	0.0	7.060092684896767E-5
3	0.0	0.0	0.0	7.060092684896767E-5	7.060092684896767E-5
4	0.0	0.0	0.0	7.060092684896767E-5	7.060092684896767E-5
5	0.0	0.0	0.0	7.060092684896767E-5	7.060092684896767E-5
6	0.0	0.0	0.0	7.060092684896767E-5	7.060092684896767E-5
7	0.0	0.0	0.0	7.060092684896767E-5	7.060092684896767E-5
8	0.0	0.0	0.0	7.060092684896767E-5	7.060092684896767E-5
9	0.0	0.0	0.0	1.4120185369793535E-4	7.060092684896767E-5
10	0.0	0.0	0.0	1.4120185369793535E-4	7.060092684896767E-5
11	0.0	0.0	0.0	1.4120185369793535E-4	7.060092684896767E-5
12	0.0	0.0	0.0	1.4120185369793535E-4	4.2360556109380604E-4
13	0.0	0.0	0.0	1.4120185369793535E-4	4.2360556109380604E-4
14	0.0	0.0	0.0	1.4120185369793535E-4	4.942064879427737E-4
15	0.0	0.0	0.0	1.4120185369793535E-4	5.648074147917414E-4
16	0.0	0.0	0.0	2.1180278054690302E-4	5.648074147917414E-4
17	0.0	0.0	0.0	2.824037073958707E-4	5.648074147917414E-4
18	0.0	0.0	0.0	3.5300463424483837E-4	7.060092684896767E-4
19	0.0	0.0	0.0	4.2360556109380604E-4	7.060092684896767E-4
20	0.0	0.0	0.0	4.942064879427737E-4	8.472111221876121E-4
21	0.0	0.0	0.0	6.354083416407091E-4	8.472111221876121E-4
22	0.0	0.0	0.0	7.766101953386444E-4	9.178120490365798E-4
23	0.0	0.0	0.0	9.884129758855474E-4	9.884129758855474E-4
24	0.0	0.0	0.0	0.0014826194638283211	9.884129758855474E-4
25	0.0	0.0	0.0	0.0018356240980731595	9.884129758855474E-4
26	0.0	0.0	0.0	0.0021886287323179977	9.884129758855474E-4
27	0.0	0.0	0.0	0.0026122342934118037	0.0011296148295834828
28	0.0	0.0	0.0	0.004024252830391158	0.0011296148295834828
29	0.0	0.0	0.0	0.008048505660782315	0.0011296148295834828
30	0.0	0.0	0.0	0.01426138722349147	0.0011296148295834828
31	0.0	0.0	0.0	0.029934792983962292	0.0011296148295834828
32	0.0	0.0	0.0	0.05069146547755879	0.0011296148295834828
33	0.0	0.0	0.0	0.07102453241006149	0.0011296148295834828
34	0.0	0.0	0.0	0.09185180583050695	0.0011296148295834828
35	0.0	0.0	0.0	0.11571491910545802	0.0011296148295834828
36	0.0	0.0	0.0	0.15214499735952533	0.0012002157564324505
37	0.0	0.0	0.0	0.2081315323507567	0.0012002157564324505
38	0.0	0.0	0.0	0.2737903943202966	0.0012002157564324505
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTACGCT	65	1.8143863E-5	23.691109	3
GTATCAA	2225	0.0	23.432524	1
TGTAGGA	570	0.0	22.769976	2
GTAGGAC	515	0.0	21.785309	3
TAGGACG	435	0.0	21.746027	4
TCGATCG	65	4.933246E-4	20.307383	41
ATACCGT	340	0.0	20.05781	6
AGGACGT	500	0.0	19.799	5
CTGTAGG	695	0.0	19.624907	1
ATCGTTT	345	0.0	19.130144	29
GGTATCA	1130	0.0	18.884	1
ACCGTAC	70	8.1270264E-4	18.85619	8
CAACGTA	60	0.0074133673	18.333054	20
ACGATTA	60	0.007414877	18.332405	40
GGTATAT	120	3.164605E-7	18.332405	1
CGTCGTA	370	0.0	17.837566	10
ACCGTCG	360	0.0	17.721327	8
GTCCTAC	435	0.0	17.700254	1
TACCGTC	375	0.0	17.59911	7
CCTTATA	125	4.8583024E-7	17.59849	2
>>END_MODULE