FastQCFastQC Report
Thu 2 Feb 2017
SRR4063991_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4063991_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3100795
Sequences flagged as poor quality0
Sequence length25
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT50060.16144246878623064No Hit
TATCAACGCAGAGTACTTTTTTTTT32140.10365083793027273No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA9150.014.2221581
GTATCAA31100.013.4998191
CGAACGA1650.012.09164816
GCGTTAT2200.012.0893081
CGTCGTA3400.012.01563510
ACGAACG1850.011.29835215
CCGTCGT3450.011.2903639
TACCGTC3950.011.0634317
CGTTATT2150.011.0434442
ATTAGAG3700.011.0376523
TTAGAGT2850.010.9981884
TTTGCGC2650.010.75520117
GTATTAG3450.010.7377081
TTGCGCG2350.010.51179118
CGAGACT1451.449007E-810.4810314
TAACGAA2001.8189894E-1210.45114513
TCGCGTA2550.010.4318429
ACCGTCG3700.010.2707328
ATAACGA2250.010.13444312
ATACCGT4500.010.1331366
Produced by FastQC (version 0.11.5)