FastQCFastQC Report
Thu 2 Feb 2017
SRR4063991_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4063991_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3100795
Sequences flagged as poor quality0
Sequence length50
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA31500.028.9118291
GGTATCA12300.025.7539081
TCAACGC44200.019.858044
CAACGCA45650.019.2754675
AACGCAG47350.018.6763426
ATCAACG47100.018.6353593
TATCAAC48550.018.3500612
ATACCGT5900.017.8967846
ACCGTCG5550.017.439968
TATACCG1551.4430043E-817.0308115
TACCGTC5750.016.8333517
CGTCGTA5600.016.49912810
CCGTCGT6250.016.1916689
TACCGTA1704.5021807E-815.5280917
CGCGATT1004.9487804E-415.39918812
TATACTG4000.015.3986915
ACGCAGA57250.015.3314487
GCATATA2455.456968E-1215.2640071
AGAGTAC58200.015.15727511
GTAGGAC5750.014.920473
Produced by FastQC (version 0.11.5)