##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4063991_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3100795
Sequences flagged as poor quality	0
Sequence length	50
%GC	51
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.278032891564905	32.0	32.0	32.0	32.0	32.0
2	31.316851968608052	32.0	32.0	32.0	32.0	32.0
3	31.41327885268133	32.0	32.0	32.0	32.0	32.0
4	31.516602032704515	32.0	32.0	32.0	32.0	32.0
5	31.431064936572717	32.0	32.0	32.0	32.0	32.0
6	35.03898226100081	36.0	36.0	36.0	36.0	36.0
7	35.0597940205657	36.0	36.0	36.0	36.0	36.0
8	34.99238711362731	36.0	36.0	36.0	36.0	36.0
9	35.12623117619836	36.0	36.0	36.0	36.0	36.0
10	34.95734771244149	36.0	36.0	36.0	36.0	36.0
11	35.122928474794364	36.0	36.0	36.0	36.0	36.0
12	35.0286100822531	36.0	36.0	36.0	36.0	36.0
13	35.07762331918105	36.0	36.0	36.0	36.0	36.0
14	35.0165805865915	36.0	36.0	36.0	36.0	36.0
15	34.977211328062644	36.0	36.0	36.0	36.0	36.0
16	35.000302503067765	36.0	36.0	36.0	36.0	36.0
17	34.96113867572671	36.0	36.0	36.0	36.0	36.0
18	34.95847161776254	36.0	36.0	36.0	36.0	36.0
19	34.95534177525441	36.0	36.0	36.0	36.0	36.0
20	34.95467065704118	36.0	36.0	36.0	36.0	36.0
21	34.95446103338015	36.0	36.0	36.0	36.0	36.0
22	34.933460290022396	36.0	36.0	36.0	36.0	36.0
23	34.88002915381378	36.0	36.0	36.0	32.0	36.0
24	34.85314443553992	36.0	36.0	36.0	32.0	36.0
25	34.829589185999076	36.0	36.0	36.0	32.0	36.0
26	34.766692090254274	36.0	36.0	36.0	32.0	36.0
27	34.76043305023389	36.0	36.0	36.0	32.0	36.0
28	34.74380150896786	36.0	36.0	36.0	32.0	36.0
29	34.718109065578346	36.0	36.0	36.0	32.0	36.0
30	34.69575157338682	36.0	36.0	36.0	32.0	36.0
31	34.69823319503546	36.0	36.0	36.0	32.0	36.0
32	34.65699119096877	36.0	36.0	36.0	32.0	36.0
33	34.637193042429445	36.0	36.0	36.0	32.0	36.0
34	34.63541220880452	36.0	36.0	36.0	32.0	36.0
35	34.59629062869361	36.0	36.0	36.0	32.0	36.0
36	34.57591972381277	36.0	36.0	36.0	32.0	36.0
37	34.56951136724614	36.0	36.0	36.0	32.0	36.0
38	34.53721126356305	36.0	36.0	36.0	32.0	36.0
39	34.53373796074877	36.0	36.0	36.0	32.0	36.0
40	34.51875051398109	36.0	36.0	36.0	32.0	36.0
41	34.50442838046372	36.0	36.0	36.0	32.0	36.0
42	34.452632953807004	36.0	36.0	36.0	32.0	36.0
43	34.444913643114106	36.0	36.0	36.0	32.0	36.0
44	34.38931112827517	36.0	36.0	36.0	32.0	36.0
45	34.36738513832743	36.0	36.0	36.0	32.0	36.0
46	34.354220127418934	36.0	36.0	36.0	32.0	36.0
47	34.335243703630844	36.0	36.0	36.0	32.0	36.0
48	34.293076775472095	36.0	36.0	36.0	32.0	36.0
49	34.285061089172295	36.0	36.0	36.0	32.0	36.0
50	33.65552769531685	36.0	36.0	36.0	27.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	1.0
18	2.0
19	9.0
20	21.0
21	81.0
22	254.0
23	899.0
24	2282.0
25	5668.0
26	10973.0
27	20818.0
28	34397.0
29	53873.0
30	78931.0
31	114001.0
32	166622.0
33	273071.0
34	567218.0
35	1771674.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.62120486435318	17.58343371988202	11.808264110962211	26.98709730480259
2	16.43767052161069	19.57301066183347	36.036094144054076	27.953224672501758
3	18.474559490296482	22.955588959304794	28.493609517338044	30.07624203306068
4	12.862636839907186	15.565459825625364	34.59361228330154	36.97829105116591
5	14.981093558264897	35.97909568352632	33.09354536497898	15.9462653932298
6	35.88367101050312	34.56526590625879	15.882103668998113	13.668959414239975
7	31.170671502418895	29.86442500353942	20.042614905283905	18.92228858875778
8	28.784811637015668	32.869280297472095	18.41227814157337	19.933629923938863
9	27.326145210066038	13.905609683810082	17.63775561047394	41.13048949564994
10	15.898007137591163	26.610263399430977	31.495249883415905	25.996479579561953
11	37.92615697785793	20.579535435117585	21.854370203244716	19.639937383779767
12	24.907168818268893	22.91777256763599	28.417467806591223	23.757590807503895
13	29.96331888319641	19.088564224849378	24.69412515181674	26.25399174013748
14	23.980145679192308	18.60824965024997	24.49108064858394	32.92052402197378
15	25.699885056433292	26.607855380094254	21.497962364044724	26.194297199427734
16	26.33050059517494	25.303432793607005	22.9284412995615	25.437625311656557
17	24.86265618978359	25.510909299066853	24.365751363763163	25.2606831473864
18	25.488842850471748	24.78005619209544	24.98090810347808	24.75019285395473
19	26.05164999674277	24.569964983162375	24.58402589788293	24.794359122211922
20	26.313156852551558	24.0031192232128	24.22483820158335	25.458885722652298
21	26.935285950860987	23.904837307851697	24.01097137992031	25.148905361367003
22	26.48511961774952	23.671582181854067	24.3185777578259	25.524720442570516
23	25.393215451204064	23.757434014204808	24.69555250757793	26.153798027013192
24	25.486467092904192	24.292314018269625	24.432827277051704	25.788391611774475
25	25.636225312013323	23.890582565696224	24.635402607563528	25.83778951472693
26	25.499918243133635	24.46726613101675	24.724243926367865	25.308571699481753
27	25.85948493311148	24.298532513149844	24.251509462502018	25.590473091236653
28	25.560135196150526	24.074332081994918	24.804300992040456	25.561231729814104
29	25.483694109755156	24.287262313269174	24.653564333445136	25.575479243530534
30	25.468508924470957	24.411388698250196	24.66852517892803	25.451577198350815
31	25.831849730371285	24.080391929199887	24.222914570271175	25.86484377015765
32	25.728534989900698	24.263773220898834	24.025569635847802	25.98212215335267
33	25.348238914584027	24.151079459201277	24.376740146413375	26.12394147980132
34	25.947570852944384	23.96393351475368	24.554183901922073	25.534311730379866
35	25.945499667012285	24.0059792660725	24.795861599663304	25.25265946725191
36	25.443975533491475	24.349207321891335	24.439577947890143	25.767239196727047
37	26.0724576077134	24.023037512392435	24.385119144755897	25.51938573513827
38	25.43504448411489	24.037419227909933	24.546942553647828	25.98059373432735
39	26.017382655465244	24.010208093264794	24.152435097238442	25.81997415403152
40	26.072969678205848	24.151783122738443	24.45422705547708	25.321020143578632
41	25.37294034212308	24.397288738628767	24.73224568540557	25.497525233842584
42	26.02052087482202	24.32689563314623	24.48463191910331	25.167951572928445
43	25.69451633553764	23.682199980327372	24.655640319857582	25.96764336427741
44	25.397192433864685	24.058683601847804	24.49025465981932	26.05386930446819
45	25.494224174712787	24.25572495081059	24.47012337907145	25.77992749540517
46	25.506728973771033	24.102916372384925	24.54361336055238	25.846741293291664
47	25.595980047298493	24.005614156394632	24.78984807785772	25.608557718449156
48	25.822175236991967	24.537610078632895	24.15202445353332	25.488190230841816
49	25.438904845416616	24.396846476990028	24.375464801505167	25.78878387608819
50	25.336716597549046	24.514754964358655	24.4218742995653	25.726654138526996
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	10.0
1	25.5
2	41.0
3	143.0
4	245.0
5	310.0
6	375.0
7	365.0
8	355.0
9	459.0
10	563.0
11	827.0
12	1091.0
13	1978.5
14	2866.0
15	3747.0
16	4628.0
17	4526.5
18	4425.0
19	4382.0
20	4339.0
21	4832.0
22	5325.0
23	6495.5
24	7666.0
25	9982.0
26	12298.0
27	15266.5
28	18235.0
29	21813.0
30	25391.0
31	30103.0
32	34815.0
33	40961.5
34	47108.0
35	56445.5
36	65783.0
37	78274.5
38	90766.0
39	103271.5
40	115777.0
41	130034.0
42	144291.0
43	154362.0
44	164433.0
45	181326.5
46	198220.0
47	213062.5
48	227905.0
49	240289.5
50	252674.0
51	252833.5
52	252993.0
53	255153.5
54	257314.0
55	254344.0
56	251374.0
57	241804.5
58	232235.0
59	214930.5
60	197626.0
61	174342.0
62	151058.0
63	130211.0
64	109364.0
65	92374.0
66	75384.0
67	63580.0
68	51776.0
69	43976.5
70	36177.0
71	27978.5
72	19780.0
73	16685.0
74	13590.0
75	10992.5
76	8395.0
77	7096.5
78	5798.0
79	4743.0
80	3688.0
81	2912.5
82	2137.0
83	1688.5
84	1240.0
85	919.0
86	598.0
87	443.5
88	289.0
89	214.0
90	139.0
91	110.0
92	81.0
93	64.0
94	47.0
95	42.5
96	38.0
97	33.0
98	28.0
99	24.5
100	21.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.009029942321243422
2	4.837469100666119E-4
3	3.869975280532896E-4
4	0.0
5	0.0
6	9.67493820133224E-5
7	6.44995880088816E-5
8	0.0
9	0.0010319934081421055
10	9.352440261287831E-4
11	3.22497940044408E-5
12	1.6124897002220398E-4
13	5.482464980754935E-4
14	2.902481460399672E-4
15	0.004611720542635034
16	8.384946441154608E-4
17	0.0
18	9.67493820133224E-5
19	3.22497940044408E-5
20	7.417452621021383E-4
21	0.0
22	3.22497940044408E-5
23	4.5149711606217114E-4
24	6.449958800888159E-4
25	0.0014834905242042766
26	0.00445047157261283
27	0.00606296127283487
28	0.0037087263105106912
29	0.0024187345503330596
30	0.0034507279584751653
31	0.0075787015910435875
32	0.0027734822843819085
33	0.003934474868541777
34	0.002483234138341941
35	0.0025799835203552638
36	0.0074174526210213825
37	0.00364422672250181
38	0.0080624485011102
39	0.0038054756925240143
40	0.0017737386702442438
41	0.003160479812435198
42	0.00161248970022204
43	0.0012899917601776319
44	9.997436141376648E-4
45	8.384946441154608E-4
46	8.0624485011102E-4
47	0.0019994872282753295
48	0.0018382382582531256
49	2.902481460399672E-4
50	0.0013544913481865135
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	3100795.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	48.13550992177838
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.59643925647073	34.4633111219385
2	14.694697503549694	14.146735149592965
3	5.469040486496018	7.897651578010103
4	2.544269261998965	4.898787932185078
5	1.4543558804205945	3.500308095589113
6	0.8976080077586801	2.5924091498001403
7	0.6117143257015648	2.061162669587115
8	0.42518378932893386	1.637315080785778
9	0.3195118487515183	1.3841879189134016
>10	1.821405965757246	15.745245308729796
>50	0.1068071453269284	3.549223652602198
>100	0.05142329685774908	4.849626968709344
>500	0.005051271148868853	1.6187331444988167
>1k	0.0024919604324366747	1.6553022290575845
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	2.2574855803108557E-4
2	0.0	3.22497940044408E-5	0.0	0.0	2.2574855803108557E-4
3	0.0	3.22497940044408E-5	0.0	0.0	2.2574855803108557E-4
4	0.0	3.22497940044408E-5	0.0	0.0	2.2574855803108557E-4
5	0.0	3.22497940044408E-5	0.0	3.22497940044408E-5	3.2249794004440797E-4
6	0.0	3.22497940044408E-5	0.0	3.22497940044408E-5	3.8699752805328955E-4
7	0.0	3.22497940044408E-5	0.0	3.22497940044408E-5	3.8699752805328955E-4
8	0.0	3.22497940044408E-5	0.0	3.22497940044408E-5	3.8699752805328955E-4
9	0.0	3.22497940044408E-5	0.0	3.22497940044408E-5	4.5149711606217114E-4
10	0.0	3.22497940044408E-5	0.0	6.44995880088816E-5	4.837469100666119E-4
11	0.0	3.22497940044408E-5	0.0	6.44995880088816E-5	4.837469100666119E-4
12	0.0	3.22497940044408E-5	0.0	6.44995880088816E-5	9.997436141376648E-4
13	0.0	3.22497940044408E-5	0.0	6.44995880088816E-5	0.0010642432021465463
14	0.0	3.22497940044408E-5	0.0	6.44995880088816E-5	0.0010642432021465463
15	0.0	3.22497940044408E-5	0.0	9.674938201332239E-5	0.0012899917601776319
16	0.0	3.22497940044408E-5	0.0	9.674938201332239E-5	0.0013867411421909543
17	0.0	3.22497940044408E-5	0.0	9.674938201332239E-5	0.001418990936195395
18	0.0	3.22497940044408E-5	0.0	1.289991760177632E-4	0.001418990936195395
19	0.0	3.22497940044408E-5	0.0	1.289991760177632E-4	0.001418990936195395
20	0.0	3.22497940044408E-5	0.0	1.289991760177632E-4	0.0016447394942264806
21	0.0	3.22497940044408E-5	0.0	1.6124897002220398E-4	0.001741488876239803
22	0.0	3.22497940044408E-5	0.0	2.579983520355264E-4	0.0018382382582531253
23	0.0	3.22497940044408E-5	0.0	3.8699752805328955E-4	0.0018704880522575661
24	0.0	3.22497940044408E-5	0.0	4.1924732205773034E-4	0.0018704880522575661
25	0.0	3.22497940044408E-5	0.0	5.159967040710528E-4	0.0018704880522575661
26	0.0	3.22497940044408E-5	0.0	7.094954680976975E-4	0.001902737846262007
27	0.0	3.22497940044408E-5	0.0	9.997436141376648E-4	0.0019672374342708885
28	0.0	3.22497940044408E-5	0.0	0.0029669810484085533	0.0019672374342708885
29	0.0	3.22497940044408E-5	0.0	0.007094954680976975	0.0019994872282753295
30	0.0	3.22497940044408E-5	0.0	0.01622164638423372	0.0019994872282753295
31	0.0	3.22497940044408E-5	0.0	0.03544252361088043	0.002063986816284211
32	0.0	3.22497940044408E-5	0.0	0.05595339259770478	0.0021284864042930927
33	0.0	3.22497940044408E-5	0.0	0.07926999366291548	0.0021607361982975332
34	0.0	3.22497940044408E-5	0.0	0.10203834823005067	0.002257485580310856
35	0.0	3.22497940044408E-5	0.0	0.1298054208678742	0.002257485580310856
36	0.0	3.22497940044408E-5	0.0	0.16818267573315876	0.002257485580310856
37	0.0	6.44995880088816E-5	0.0	0.22897353743152965	0.002257485580310856
38	0.0	6.44995880088816E-5	0.0	0.312919751225089	0.0024187345503330596
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATCAA	3150	0.0	28.911829	1
GGTATCA	1230	0.0	25.753908	1
TCAACGC	4420	0.0	19.85804	4
CAACGCA	4565	0.0	19.275467	5
AACGCAG	4735	0.0	18.676342	6
ATCAACG	4710	0.0	18.635359	3
TATCAAC	4855	0.0	18.350061	2
ATACCGT	590	0.0	17.896784	6
ACCGTCG	555	0.0	17.43996	8
TATACCG	155	1.4430043E-8	17.030811	5
TACCGTC	575	0.0	16.833351	7
CGTCGTA	560	0.0	16.499128	10
CCGTCGT	625	0.0	16.191668	9
TACCGTA	170	4.5021807E-8	15.528091	7
CGCGATT	100	4.9487804E-4	15.399188	12
TATACTG	400	0.0	15.398691	5
ACGCAGA	5725	0.0	15.331448	7
GCATATA	245	5.456968E-12	15.264007	1
AGAGTAC	5820	0.0	15.157275	11
GTAGGAC	575	0.0	14.92047	3
>>END_MODULE