FastQCFastQC Report
Thu 2 Feb 2017
SRR4063990_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4063990_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1206467
Sequences flagged as poor quality0
Sequence length25
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT26590.2203955847942795No Hit
TATCAACGCAGAGTACTTTTTTTTT18570.15392049678938588No Hit
GTACTTTTTTTTTTTTTTTTTTTTT14960.12399841852284398No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATAAC1101.8553692E-1013.81869410
ACGAACG1051.3624231E-913.57531215
TAACGAA1051.366061E-913.57362213
AACGAAC1001.00335455E-813.30270114
ATTCCGA1153.783498E-1013.2156896
AATTCCG1102.75395E-912.9512655
CGTCGTA1403.6379788E-1212.89333610
AGACCGT604.09529E-412.6650356
GATAACG1155.311449E-912.39227811
TCGCACG701.088865E-412.21625913
TATAGGA1405.2750693E-1112.207652
AGAATTT2200.012.09386617
CTACTAA550.003074537712.0873461
ATAACGA1209.977157E-911.8769212
TCCGATA1209.997166E-911.8754418
CCGATAA1209.997166E-911.8754419
CGGACAT1201.00390025E-811.8719935
GTATAGG1201.00462785E-811.8715021
CCTATTC1850.011.8063523
CCGTCGT1459.640644E-1111.7935419
Produced by FastQC (version 0.11.5)