##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4063990_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1206467
Sequences flagged as poor quality	0
Sequence length	25
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.247339545963545	32.0	32.0	32.0	32.0	32.0
2	30.855398448527808	32.0	32.0	32.0	32.0	32.0
3	30.869887033793713	32.0	32.0	32.0	32.0	32.0
4	30.890425515161212	32.0	32.0	32.0	32.0	32.0
5	30.766795942201487	32.0	32.0	32.0	32.0	32.0
6	34.434376572256014	36.0	36.0	36.0	32.0	36.0
7	34.38313936477334	36.0	36.0	36.0	32.0	36.0
8	34.33008113773522	36.0	36.0	36.0	32.0	36.0
9	34.493695227469956	36.0	36.0	36.0	32.0	36.0
10	34.15873621077079	36.0	36.0	36.0	32.0	36.0
11	34.46900163866894	36.0	36.0	36.0	32.0	36.0
12	34.27370247176259	36.0	36.0	36.0	32.0	36.0
13	34.357347527947304	36.0	36.0	36.0	32.0	36.0
14	34.254447904501326	36.0	36.0	36.0	32.0	36.0
15	34.18130127056936	36.0	36.0	36.0	32.0	36.0
16	34.17957639952025	36.0	36.0	36.0	32.0	36.0
17	34.1060203055699	36.0	36.0	36.0	32.0	36.0
18	34.09749375656359	36.0	36.0	36.0	32.0	36.0
19	34.12053375682882	36.0	36.0	36.0	32.0	36.0
20	34.10585867661528	36.0	36.0	36.0	32.0	36.0
21	34.08440015350607	36.0	36.0	36.0	32.0	36.0
22	34.06151100693181	36.0	36.0	36.0	32.0	36.0
23	33.99388793891586	36.0	36.0	36.0	32.0	36.0
24	33.98238244394584	36.0	36.0	36.0	32.0	36.0
25	33.58264337109925	36.0	36.0	36.0	27.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
3	6.0
4	10.0
5	21.0
6	51.0
7	19.0
8	87.0
9	80.0
10	96.0
11	32.0
12	70.0
13	68.0
14	110.0
15	206.0
16	297.0
17	449.0
18	556.0
19	728.0
20	1092.0
21	1579.0
22	2538.0
23	4005.0
24	5988.0
25	9143.0
26	13098.0
27	17855.0
28	24459.0
29	33332.0
30	44496.0
31	60800.0
32	86639.0
33	121499.0
34	253846.0
35	523212.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.43501459763834	16.94661020532878	11.102248144097471	26.516127052935406
2	16.879406169569922	19.661473551585082	37.02454814096427	26.43457213788073
3	18.763070490924115	23.714667712167792	28.591582334457026	28.930679462451064
4	12.803762565162288	15.259476248144288	35.76409697855872	36.172664208134705
5	14.732062921527147	36.331080767419806	33.75043934982857	15.186416961224477
6	34.369761831732006	35.19153768994189	16.930091437382387	13.50860904094372
7	30.264427700654643	30.290958353507687	21.058456977230065	18.386156968607605
8	28.315732565621452	32.53751218897387	19.544480633627636	19.602274611777037
9	27.70598850265872	13.870782621964366	18.318870434318203	40.104358441058714
10	15.744818491702123	26.629842502052696	31.99099300838496	25.63434599786022
11	37.14094967179057	20.930845880484497	22.58644783273967	19.341756614985258
12	24.69423842850247	23.485209152028983	29.02275731994778	22.79779509952077
13	29.831273778055724	19.34475205298914	25.011756841154504	25.812217327800635
14	23.689080251455056	19.276009167044755	25.269839742606102	31.765070838894083
15	25.354476993000034	26.803486713333115	22.31538002189563	25.526656271771227
16	25.863550480980575	25.35055430128842	23.729204370710864	25.056690847020143
17	24.23926502995333	25.55624947644913	25.141638654103104	25.06284683949444
18	24.94641298829713	24.732562653668637	25.905919125088346	24.41510523294589
19	25.588509761145374	24.838724394361055	25.297521038202635	24.275244806290928
20	25.954941256398833	24.110467667584963	24.825942581168036	25.10864849484817
21	26.720971514839825	24.164606732252768	24.38873865653566	24.72568309637175
22	26.029769923484793	24.066039989647553	24.767567639310105	25.136622447557556
23	24.71685388225203	23.973660131749114	25.339348123101885	25.97013786289697
24	24.833141713824975	24.73434568159308	25.15632336082368	25.276189243758264
25	24.98977613679819	24.308738271590602	25.201138436499647	25.50034715511156
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	230.0
1	230.0
2	355.5
3	481.0
4	481.0
5	481.0
6	1322.0
7	2163.0
8	2163.0
9	2163.0
10	2242.5
11	2322.0
12	2322.0
13	2322.0
14	2672.0
15	3022.0
16	3022.0
17	3022.0
18	5186.5
19	7351.0
20	7351.0
21	7351.0
22	11718.5
23	16086.0
24	16086.0
25	16086.0
26	23867.0
27	31648.0
28	31648.0
29	31648.0
30	39914.5
31	48181.0
32	48181.0
33	48181.0
34	60409.0
35	72637.0
36	72637.0
37	72637.0
38	84330.0
39	96023.0
40	96023.0
41	96023.0
42	111172.5
43	126322.0
44	126322.0
45	126322.0
46	141401.5
47	156481.0
48	156481.0
49	156481.0
50	161616.5
51	166752.0
52	166752.0
53	166752.0
54	156605.5
55	146459.0
56	146459.0
57	146459.0
58	134249.5
59	122040.0
60	122040.0
61	122040.0
62	106160.5
63	90281.0
64	90281.0
65	90281.0
66	74109.0
67	57937.0
68	57937.0
69	57937.0
70	43710.0
71	29483.0
72	29483.0
73	29483.0
74	22269.0
75	15055.0
76	15055.0
77	15055.0
78	11918.5
79	8782.0
80	8782.0
81	8782.0
82	6096.5
83	3411.0
84	3411.0
85	3411.0
86	2554.0
87	1697.0
88	1697.0
89	1697.0
90	1205.5
91	714.0
92	714.0
93	714.0
94	505.5
95	297.0
96	297.0
97	297.0
98	454.5
99	612.0
100	612.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.03729898952893034
2	0.0051389718906526246
3	0.0023208260151334433
4	0.004641652030266887
5	0.011521243432269594
6	0.014670935881379266
7	0.02610929267025124
8	0.038044969319508945
9	0.05163837883671911
10	0.061087456184048136
11	0.05652869079717887
12	0.06539756164072454
13	0.06514890171053166
14	0.0699563270275938
15	0.061916322618024364
16	0.0667237479350865
17	0.06282807569539822
18	0.07882519787113945
19	0.07642148521260839
20	0.08073159066928479
21	0.0768359184295965
22	0.07915674444472995
23	0.08238932353723724
24	0.0789909711579347
25	0.07907385780133232
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	1206467.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	58.27485507806702
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.10735382593444	46.099695798157
2	12.67519406490405	14.772901944373174
3	3.679047468038446	6.431878740758096
4	1.5422614462319824	3.595002490866353
5	0.7946626275596986	2.3154424728498695
6	0.48241251104934574	1.6867511501548218
7	0.3281480053515232	1.3385944219211745
8	0.23072827493996828	1.0756525427631245
9	0.16709698114142907	0.876379712399935
>10	0.8602471316258237	8.903956012355797
>50	0.06309380977605296	2.588829072263703
>100	0.06332164105034119	7.346212578686756
>500	0.006003398237077159	2.4679218387842763
>1k	4.2881415979122557E-4	0.5007812236658252
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	2659	0.2203955847942795	No Hit
TATCAACGCAGAGTACTTTTTTTTT	1857	0.15392049678938588	No Hit
GTACTTTTTTTTTTTTTTTTTTTTT	1496	0.12399841852284398	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	8.288664339762297E-5	0.0
12	0.0	0.0	0.0	8.288664339762297E-5	8.288664339762297E-5
13	0.0	0.0	0.0	8.288664339762297E-5	8.288664339762297E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGATAAC	110	1.8553692E-10	13.818694	10
ACGAACG	105	1.3624231E-9	13.575312	15
TAACGAA	105	1.366061E-9	13.573622	13
AACGAAC	100	1.00335455E-8	13.302701	14
ATTCCGA	115	3.783498E-10	13.215689	6
AATTCCG	110	2.75395E-9	12.951265	5
CGTCGTA	140	3.6379788E-12	12.893336	10
AGACCGT	60	4.09529E-4	12.665035	6
GATAACG	115	5.311449E-9	12.392278	11
TCGCACG	70	1.088865E-4	12.216259	13
TATAGGA	140	5.2750693E-11	12.20765	2
AGAATTT	220	0.0	12.093866	17
CTACTAA	55	0.0030745377	12.087346	1
ATAACGA	120	9.977157E-9	11.87692	12
TCCGATA	120	9.997166E-9	11.875441	8
CCGATAA	120	9.997166E-9	11.875441	9
CGGACAT	120	1.00390025E-8	11.871993	5
GTATAGG	120	1.00462785E-8	11.871502	1
CCTATTC	185	0.0	11.806352	3
CCGTCGT	145	9.640644E-11	11.793541	9
>>END_MODULE