FastQCFastQC Report
Thu 2 Feb 2017
SRR4063990_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4063990_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1206467
Sequences flagged as poor quality0
Sequence length50
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACCG407.0356095E-427.497675
TAACGGC450.001397552324.44642436
TAGCGAG450.001398902924.4423715
ACGTACT550.00448580719.99830419
GTATCAA21200.019.924491
CGAACGT1605.2750693E-1119.248374
GTATAAA1254.8525544E-717.5999661
TATAATA1751.9099389E-1017.5992362
GTATAGA1154.272948E-617.217361
AACCGTG1307.3200863E-716.9223447
CTACACC2109.094947E-1216.7604854
GATTAAT3350.016.4178838
GATATAT2151.2732926E-1116.3720631
TAGTACT1259.447751E-615.8386574
TAGCACT2101.5097612E-1015.7129544
CGCGCAA1704.4896296E-815.52938421
GTACATG41650.015.4765621
AACGCAG27600.015.4624576
ATAATAC1857.574272E-915.458153
TACATGG42200.015.4306112