##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4063990_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1206467
Sequences flagged as poor quality	0
Sequence length	50
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.24700717052352	32.0	32.0	32.0	32.0	32.0
2	31.34454319927524	32.0	32.0	32.0	32.0	32.0
3	31.438828413872905	32.0	32.0	32.0	32.0	32.0
4	31.536346207563074	32.0	32.0	32.0	32.0	32.0
5	31.459495369537667	32.0	32.0	32.0	32.0	32.0
6	35.06337595640826	36.0	36.0	36.0	36.0	36.0
7	35.084006441949924	36.0	36.0	36.0	36.0	36.0
8	35.025080669425684	36.0	36.0	36.0	36.0	36.0
9	35.15653308378928	36.0	36.0	36.0	36.0	36.0
10	34.98132812584182	36.0	36.0	36.0	36.0	36.0
11	35.15225779072283	36.0	36.0	36.0	36.0	36.0
12	35.0495935653441	36.0	36.0	36.0	36.0	36.0
13	35.09526244812332	36.0	36.0	36.0	36.0	36.0
14	35.04127423294628	36.0	36.0	36.0	36.0	36.0
15	35.0106608800738	36.0	36.0	36.0	36.0	36.0
16	35.02386389350061	36.0	36.0	36.0	36.0	36.0
17	34.9955721955097	36.0	36.0	36.0	36.0	36.0
18	34.98068989868766	36.0	36.0	36.0	36.0	36.0
19	34.98217108300517	36.0	36.0	36.0	36.0	36.0
20	34.976805830578044	36.0	36.0	36.0	36.0	36.0
21	34.974696365503576	36.0	36.0	36.0	36.0	36.0
22	34.956404941038585	36.0	36.0	36.0	36.0	36.0
23	34.90189039567597	36.0	36.0	36.0	32.0	36.0
24	34.88119442968602	36.0	36.0	36.0	32.0	36.0
25	34.8447715519778	36.0	36.0	36.0	32.0	36.0
26	34.784976298564324	36.0	36.0	36.0	32.0	36.0
27	34.78474338709637	36.0	36.0	36.0	32.0	36.0
28	34.762596904846966	36.0	36.0	36.0	32.0	36.0
29	34.73712252386514	36.0	36.0	36.0	32.0	36.0
30	34.712907190996525	36.0	36.0	36.0	32.0	36.0
31	34.712525083570455	36.0	36.0	36.0	32.0	36.0
32	34.66395185280658	36.0	36.0	36.0	32.0	36.0
33	34.641385135275144	36.0	36.0	36.0	32.0	36.0
34	34.62557699464635	36.0	36.0	36.0	32.0	36.0
35	34.593648230743156	36.0	36.0	36.0	32.0	36.0
36	34.55994900813698	36.0	36.0	36.0	32.0	36.0
37	34.549318796121234	36.0	36.0	36.0	32.0	36.0
38	34.51457022860965	36.0	36.0	36.0	32.0	36.0
39	34.514572715208956	36.0	36.0	36.0	32.0	36.0
40	34.48930472196919	36.0	36.0	36.0	32.0	36.0
41	34.472417397243355	36.0	36.0	36.0	32.0	36.0
42	34.4040135370466	36.0	36.0	36.0	32.0	36.0
43	34.40015101946427	36.0	36.0	36.0	32.0	36.0
44	34.33431996067858	36.0	36.0	36.0	32.0	36.0
45	34.301436342643434	36.0	36.0	36.0	32.0	36.0
46	34.292133974654924	36.0	36.0	36.0	32.0	36.0
47	34.25116642228922	36.0	36.0	36.0	32.0	36.0
48	34.20370138594756	36.0	36.0	36.0	32.0	36.0
49	34.18653887756565	36.0	36.0	36.0	32.0	36.0
50	33.58319788274358	36.0	36.0	36.0	27.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	3.0
19	2.0
20	11.0
21	32.0
22	106.0
23	354.0
24	929.0
25	2195.0
26	4496.0
27	8301.0
28	13871.0
29	21458.0
30	31051.0
31	43903.0
32	64601.0
33	104335.0
34	216974.0
35	693845.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.50314706791748	18.024538260106223	11.655714997417855	26.816599674558443
2	16.122845145557836	20.566996722648536	36.447563205471866	26.86259492632176
3	18.03584690467349	24.365832714444856	28.88391199405867	28.71440838682298
4	12.47443983134226	16.027790233798356	35.85220316842483	35.645566766434555
5	14.328862704077277	36.92094354839378	33.380109029090725	15.370084718438218
6	34.266529516446006	35.76214106856157	16.61244480767334	13.35888460731908
7	30.180626723007524	30.53388988997618	20.981776527477773	18.303706859538522
8	28.3317322396717	32.79575819313748	19.31051574556121	19.5619938216296
9	27.815869395116906	13.703947455671525	18.099495378167976	40.3806877710436
10	15.631882272881366	26.90096821172182	32.004780964995675	25.462368550401138
11	37.38469431820348	20.8696135078705	22.481924495241064	19.263767678684953
12	24.72005012988348	23.524160647709923	29.01300160965995	22.74278761274665
13	29.90965303449762	19.362846675397442	24.949190192795452	25.778310097309483
14	23.638603090190085	19.217083325390004	25.34234369392182	31.801969890498093
15	25.494753437681585	26.890963180811816	22.180122993114278	25.43416038839233
16	25.874486406824644	25.496766963598187	23.586453158893317	25.042293470683845
17	24.33120839608543	25.626892405677072	25.07014282197524	24.97175637626226
18	25.042044389260177	24.77829821552754	25.863453748685206	24.31620364652708
19	25.739369580767647	24.77821606392881	25.28805180746759	24.194362547835954
20	25.960723139827973	24.07529128583518	24.95687800931816	25.00710756501868
21	26.82460440277272	24.150349740191814	24.439043919145735	24.586001937889723
22	26.024665407342262	24.104264766462737	24.861185593969832	25.009884232225165
23	24.74592876804981	23.93512221362954	25.339072471119888	25.97987654720076
24	25.08775667012858	24.59582199507485	25.09173524879793	25.224686085998638
25	25.110801108384084	24.231686423123882	25.34371759580452	25.313794872687517
26	24.970283241737746	24.84146917663442	25.417651008380375	24.77059657324746
27	25.484071781634494	24.52003139969877	24.713005261190286	25.28289155747645
28	24.924734997563007	24.533821174332974	25.358420562266037	25.183023265837978
29	24.96371528914625	24.60207007280151	25.25332986304231	25.180884775009925
30	24.957311291039183	24.750416111298996	25.422410991969553	24.86986160569227
31	25.377767719998047	24.5432759459632	24.65078919031834	25.42816714372042
32	25.10479360819085	24.668875407300447	24.553409719177168	25.67292126533153
33	24.9038467914669	24.28084031273002	25.09532430156299	25.71998859424009
34	25.381436889984872	24.46443003087635	25.11751662971175	25.036616449427022
35	25.827385299161737	24.3158068369168	25.152082067536952	24.704725796384512
36	25.09798037739853	24.73366247666542	24.86587796054922	25.302479185386833
37	25.708817310186994	24.628919994927102	24.712473609739966	24.94978908514594
38	24.987918310370524	24.52670032684804	25.220765419593143	25.26461594318829
39	25.403677694444998	24.47637946697966	24.687087618782368	25.43285521979297
40	25.598771249696416	24.667261811511494	25.048552380620826	24.685414558171264
41	24.8446427550101	24.847543917381525	25.37290297767017	24.934910349938207
42	25.78661184973973	24.884204767746425	24.900533801929644	24.428649580584196
43	25.260228009303386	24.26208636635661	25.1358954678302	25.341790156509802
44	25.115213866776298	24.68950277342157	24.734179229675114	25.461104130127016
45	25.152410594763957	24.84290622448599	24.82690881059642	25.17777437015363
46	24.999108959720832	24.6024095386898	24.871628034199368	25.526853467389998
47	24.98731805974603	24.58547461954179	25.301382580153174	25.125824740559
48	25.526008666792656	24.962451634721543	24.56467104979593	24.94686864868987
49	24.957458218707444	25.004703840405945	24.663706493620598	25.374131447266013
50	25.061627292062806	25.135895017265586	24.677523900779647	25.12495378989196
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	70.0
1	56.0
2	42.0
3	119.5
4	197.0
5	228.0
6	259.0
7	241.5
8	224.0
9	293.5
10	363.0
11	546.5
12	730.0
13	1367.0
14	2004.0
15	2516.0
16	3028.0
17	3139.5
18	3251.0
19	3317.5
20	3384.0
21	3677.5
22	3971.0
23	4509.5
24	5048.0
25	5738.0
26	6428.0
27	8131.5
28	9835.0
29	11603.0
30	13371.0
31	15335.0
32	17299.0
33	20222.0
34	23145.0
35	27021.0
36	30897.0
37	36184.0
38	41471.0
39	45742.0
40	50013.0
41	55047.5
42	60082.0
43	62669.0
44	65256.0
45	71696.0
46	78136.0
47	83744.5
48	89353.0
49	92367.0
50	95381.0
51	93832.0
52	92283.0
53	91182.5
54	90082.0
55	89503.0
56	88924.0
57	86861.5
58	84799.0
59	77196.0
60	69593.0
61	61400.0
62	53207.0
63	46075.5
64	38944.0
65	33136.0
66	27328.0
67	23446.5
68	19565.0
69	17248.5
70	14932.0
71	11692.5
72	8453.0
73	7105.5
74	5758.0
75	4535.0
76	3312.0
77	2846.5
78	2381.0
79	2004.5
80	1628.0
81	1272.0
82	916.0
83	763.0
84	610.0
85	425.0
86	240.0
87	178.0
88	116.0
89	80.5
90	45.0
91	41.0
92	37.0
93	31.0
94	25.0
95	22.5
96	20.0
97	16.5
98	13.0
99	15.5
100	18.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.008785984200148035
2	4.1443321698811483E-4
3	2.4865993019286894E-4
4	0.0
5	0.0
6	3.315465735904919E-4
7	8.288664339762297E-5
8	0.0
9	0.0015748462245548365
10	0.0013261862943619676
11	0.0
12	8.288664339762297E-5
13	5.802065037833608E-4
14	3.315465735904919E-4
15	0.004807425317062133
16	0.0013261862943619676
17	0.0
18	3.315465735904919E-4
19	0.0
20	4.973198603857379E-4
21	0.0
22	0.0
23	0.0011604130075667217
24	4.973198603857379E-4
25	0.0021550527283381972
26	0.006382271541616969
27	0.007128251332195575
28	0.005221858534050248
29	0.0029839191623144273
30	0.004890311960459756
31	0.008620210913352789
32	0.003978558883085903
33	0.004890311960459756
34	0.0034812390227001652
35	0.003812785596290657
36	0.008537324269955167
37	0.004641652030266887
38	0.007957117766171806
39	0.004890311960459756
40	0.003149692449109673
41	0.00447587874347164
42	0.00223793937173582
43	0.0020721660849405744
44	0.0012432996509643447
45	0.0018235061547477057
46	9.946397207714758E-4
47	0.00223793937173582
48	0.0019063927981453285
49	4.973198603857379E-4
50	0.0017406195113500826
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	1206467.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	61.3004047271698
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.82627984897476	48.933832626064714
2	12.50794088027734	15.33483676529027
3	3.534843965135258	6.500620971305551
4	1.4459887649816934	3.5455878609727285
5	0.7213758891015354	2.211031698117304
6	0.4363057352788571	1.6047430894427606
7	0.2983637599365539	1.2802873468021618
8	0.20760488277268793	1.0181010669841928
9	0.15535212817584385	0.8570833499165746
>10	0.7548552282405532	8.169769543397855
>50	0.05517032027548708	2.3645213751120377
>100	0.05183695503830488	6.408874357041248
>500	0.0039455870841467984	1.6844721798839015
>1k	1.360547270395448E-4	0.08623776966876043
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	8.288664339762297E-5
2	0.0	0.0	0.0	0.0	8.288664339762297E-5
3	0.0	0.0	0.0	0.0	8.288664339762297E-5
4	0.0	0.0	0.0	0.0	8.288664339762297E-5
5	0.0	0.0	0.0	0.0	8.288664339762297E-5
6	0.0	0.0	0.0	0.0	8.288664339762297E-5
7	0.0	0.0	0.0	0.0	8.288664339762297E-5
8	0.0	0.0	0.0	0.0	8.288664339762297E-5
9	0.0	0.0	0.0	8.288664339762297E-5	8.288664339762297E-5
10	0.0	0.0	0.0	8.288664339762297E-5	8.288664339762297E-5
11	0.0	0.0	0.0	8.288664339762297E-5	8.288664339762297E-5
12	0.0	0.0	0.0	8.288664339762297E-5	8.288664339762297E-5
13	0.0	0.0	0.0	8.288664339762297E-5	8.288664339762297E-5
14	0.0	0.0	0.0	8.288664339762297E-5	8.288664339762297E-5
15	0.0	8.288664339762297E-5	0.0	8.288664339762297E-5	8.288664339762297E-5
16	0.0	8.288664339762297E-5	0.0	8.288664339762297E-5	8.288664339762297E-5
17	0.0	8.288664339762297E-5	0.0	1.6577328679524594E-4	8.288664339762297E-5
18	0.0	8.288664339762297E-5	0.0	3.315465735904919E-4	8.288664339762297E-5
19	0.0	8.288664339762297E-5	0.0	3.315465735904919E-4	8.288664339762297E-5
20	0.0	8.288664339762297E-5	0.0	4.144332169881149E-4	8.288664339762297E-5
21	0.0	8.288664339762297E-5	0.0	4.973198603857379E-4	8.288664339762297E-5
22	0.0	8.288664339762297E-5	0.0	8.288664339762298E-4	8.288664339762297E-5
23	0.0	8.288664339762297E-5	0.0	0.0013261862943619676	8.288664339762297E-5
24	0.0	8.288664339762297E-5	0.0	0.0019063927981453285	8.288664339762297E-5
25	0.0	8.288664339762297E-5	0.0	0.0019892794415429516	8.288664339762297E-5
26	0.0	8.288664339762297E-5	0.0	0.0024865993019286894	8.288664339762297E-5
27	0.0	8.288664339762297E-5	0.0	0.003149692449109673	2.4865993019286894E-4
28	0.0	8.288664339762297E-5	0.0	0.00530474517744787	2.4865993019286894E-4
29	0.0	8.288664339762297E-5	0.0	0.009366190703931396	2.4865993019286894E-4
30	0.0	8.288664339762297E-5	0.0	0.01674310196631984	2.4865993019286894E-4
31	0.0	8.288664339762297E-5	0.0	0.04061445526483526	4.144332169881149E-4
32	0.0	8.288664339762297E-5	0.0	0.0631596222689887	4.144332169881149E-4
33	0.0	8.288664339762297E-5	0.0	0.08520746941275642	4.144332169881149E-4
34	0.0	8.288664339762297E-5	0.0	0.11032212236223618	4.144332169881149E-4
35	0.0	8.288664339762297E-5	0.0	0.14090729377595906	4.144332169881149E-4
36	0.0	8.288664339762297E-5	0.0	0.18525164799368735	4.144332169881149E-4
37	0.0	8.288664339762297E-5	0.0	0.25222405585896673	4.144332169881149E-4
38	0.0	8.288664339762297E-5	0.0	0.3477094690530284	4.144332169881149E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TATACCG	40	7.0356095E-4	27.49767	5
TAACGGC	45	0.0013975523	24.446424	36
TAGCGAG	45	0.0013989029	24.442371	5
ACGTACT	55	0.004485807	19.998304	19
GTATCAA	2120	0.0	19.92449	1
CGAACGT	160	5.2750693E-11	19.24837	4
GTATAAA	125	4.8525544E-7	17.599966	1
TATAATA	175	1.9099389E-10	17.599236	2
GTATAGA	115	4.272948E-6	17.21736	1
AACCGTG	130	7.3200863E-7	16.922344	7
CTACACC	210	9.094947E-12	16.760485	4
GATTAAT	335	0.0	16.41788	38
GATATAT	215	1.2732926E-11	16.372063	1
TAGTACT	125	9.447751E-6	15.838657	4
TAGCACT	210	1.5097612E-10	15.712954	4
CGCGCAA	170	4.4896296E-8	15.529384	21
GTACATG	4165	0.0	15.476562	1
AACGCAG	2760	0.0	15.462457	6
ATAATAC	185	7.574272E-9	15.45815	3
TACATGG	4220	0.0	15.430611	2
>>END_MODULE