FastQCFastQC Report
Thu 2 Feb 2017
SRR4063989_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4063989_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2045186
Sequences flagged as poor quality0
Sequence length25
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT42380.20721831657365147No Hit
TATCAACGCAGAGTACTTTTTTTTT31280.15294452436110945No Hit
GTTCAAAGCAGGCCCGAGCCGCCTG25470.12453635023904916No Hit
GAATAGGACCGCGGTTCTATTTTGT23400.11441502142103457No Hit
GATTAAGAGGGACGGCCGGGGGCAT23170.11329042933014405No Hit
GCGCAAGACGGACCAGAGCGAAAGC22680.11089455922346426No Hit
GTATCTGATCGTCTTCGAACCTCCG22150.10830310788358613No Hit
CTATTGGAGCTGGAATTACCGCGGC22030.1077163641839911No Hit
GTCTTGCGCCGGTCCAAGAATTTCA21730.10624950493500346No Hit
GTCCTATTCCATTATTCCTAGCTGC20530.10038206793905298No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGTAA751.4763642E-512.66896619
CGTTATT1650.012.660912
CGTCTTA1600.012.47131915
CCGAGTA550.00306652912.09221714
AAGACGG4750.011.9992445
CGCATCG3300.011.8034413
GTCCTAC8200.011.699051
CCGATAA1950.011.6924299
GTCCTAA4900.011.6304961
CCGTCGT4500.011.611239
ACCGTCG4550.011.4833548
CGTCGTA4650.011.44097710
CGAACGA2000.011.40234916
CGCAAGA4700.011.3140062
CTAGGAC2100.011.3063213
TACCGTC4750.011.19984157
CAAGACG5100.011.1754934
TAATATA3750.011.145694
GTTATTC2050.011.1188013
ACGAACA600.005877349511.08317711
Produced by FastQC (version 0.11.5)