##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4063989_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2045186
Sequences flagged as poor quality	0
Sequence length	25
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.272061807581316	32.0	32.0	32.0	32.0	32.0
2	30.90103345123622	32.0	32.0	32.0	32.0	32.0
3	30.916870641594457	32.0	32.0	32.0	32.0	32.0
4	30.943184629662046	32.0	32.0	32.0	32.0	32.0
5	30.868504869483754	32.0	32.0	32.0	32.0	32.0
6	34.52053358472041	36.0	36.0	36.0	32.0	36.0
7	34.4573535121011	36.0	36.0	36.0	32.0	36.0
8	34.42328815080878	36.0	36.0	36.0	32.0	36.0
9	34.543453749438925	36.0	36.0	36.0	32.0	36.0
10	34.2633379066745	36.0	36.0	36.0	32.0	36.0
11	34.5195361204311	36.0	36.0	36.0	32.0	36.0
12	34.33998325824644	36.0	36.0	36.0	32.0	36.0
13	34.42647759176916	36.0	36.0	36.0	32.0	36.0
14	34.327350177441076	36.0	36.0	36.0	32.0	36.0
15	34.27156894287366	36.0	36.0	36.0	32.0	36.0
16	34.26238835978732	36.0	36.0	36.0	32.0	36.0
17	34.183638065193094	36.0	36.0	36.0	32.0	36.0
18	34.189482032441056	36.0	36.0	36.0	32.0	36.0
19	34.19521843001077	36.0	36.0	36.0	32.0	36.0
20	34.179563130199405	36.0	36.0	36.0	32.0	36.0
21	34.157327010844	36.0	36.0	36.0	32.0	36.0
22	34.13678951449893	36.0	36.0	36.0	32.0	36.0
23	34.07975900480445	36.0	36.0	36.0	32.0	36.0
24	34.0567537622495	36.0	36.0	36.0	32.0	36.0
25	33.66890786461476	36.0	36.0	36.0	27.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
3	7.0
4	22.0
5	14.0
6	88.0
7	37.0
8	97.0
9	147.0
10	189.0
11	54.0
12	124.0
13	114.0
14	217.0
15	365.0
16	679.0
17	826.0
18	1076.0
19	1466.0
20	2087.0
21	3129.0
22	4763.0
23	7029.0
24	10617.0
25	15200.0
26	21877.0
27	28586.0
28	39101.0
29	52735.0
30	69565.0
31	94657.0
32	134516.0
33	191108.0
34	418837.0
35	945857.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.72114916631978	17.050982479190402	11.116828713407413	26.111039641082407
2	16.7647474454582	19.533143642580768	37.562680830069	26.13942808189203
3	18.773460958666664	23.65123782915019	28.59414430118431	28.981156910998834
4	12.44181247775183	15.262098114919867	35.96076889676458	36.335320510563726
5	14.536192927233705	36.57097142834789	33.69815828760797	15.194677356810443
6	34.38417974662127	35.5603120508694	16.78513078487552	13.270377417633819
7	30.508942090622053	30.335274601019606	20.708931135534	18.44685217282434
8	28.222562437024934	33.10419377243869	19.26616319223658	19.407080598299796
9	27.628935980361558	13.832959747651104	18.391862843993163	40.14624142799417
10	15.979048554671218	26.322136789101965	31.61102641729502	26.087788238931804
11	37.547222980529604	20.638974259622923	22.69628664214643	19.117516117701047
12	25.03034688656356	23.32984484802583	28.969937867777112	22.669870397633503
13	29.695609798103945	19.287668605515933	25.228786823131543	25.787934773248576
14	23.544084670437126	19.88381349683406	24.9566123213668	31.615489511362014
15	25.079418353822525	27.84013879192122	21.92925075944642	25.151192094809833
16	25.810133934054125	25.45353751185923	24.093695612073198	24.64263294201345
17	23.905034312627027	26.0125233994209	25.272450967632714	24.80999132031936
18	24.861125791260026	24.589640903983998	26.55203427716904	23.997199027586934
19	25.864548484345818	24.61158746725816	25.106831490297456	24.417032558098565
20	26.009601033505746	24.1768410544499	24.85752594774728	24.956031964297072
21	26.944435881288232	23.94341662103728	24.184212573747125	24.927934923927367
22	26.22067199166947	24.427679640550075	24.661192904367706	24.690455463412746
23	24.354268281642337	24.274061064167242	25.4794204135443	25.89225024064612
24	25.13995294449251	24.727385365815465	25.327274577890176	24.80538711180185
25	24.896294596259704	24.48866641970826	25.265166288317904	25.349872695714133
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	168.0
1	168.0
2	395.0
3	622.0
4	622.0
5	622.0
6	1765.5
7	2909.0
8	2909.0
9	2909.0
10	3115.5
11	3322.0
12	3322.0
13	3322.0
14	3939.5
15	4557.0
16	4557.0
17	4557.0
18	8019.0
19	11481.0
20	11481.0
21	11481.0
22	19426.5
23	27372.0
24	27372.0
25	27372.0
26	42605.0
27	57838.0
28	57838.0
29	57838.0
30	73820.5
31	89803.0
32	89803.0
33	89803.0
34	110135.5
35	130468.0
36	130468.0
37	130468.0
38	148809.5
39	167151.0
40	167151.0
41	167151.0
42	191629.0
43	216107.0
44	216107.0
45	216107.0
46	240551.0
47	264995.0
48	264995.0
49	264995.0
50	271097.0
51	277199.0
52	277199.0
53	277199.0
54	256169.0
55	235139.0
56	235139.0
57	235139.0
58	215917.5
59	196696.0
60	196696.0
61	196696.0
62	173756.0
63	150816.0
64	150816.0
65	150816.0
66	124954.5
67	99093.0
68	99093.0
69	99093.0
70	74808.5
71	50524.0
72	50524.0
73	50524.0
74	39154.5
75	27785.0
76	27785.0
77	27785.0
78	22625.0
79	17465.0
80	17465.0
81	17465.0
82	12124.5
83	6784.0
84	6784.0
85	6784.0
86	5214.5
87	3645.0
88	3645.0
89	3645.0
90	2584.0
91	1523.0
92	1523.0
93	1523.0
94	1042.0
95	561.0
96	561.0
97	561.0
98	862.0
99	1163.0
100	1163.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.03994746688076292
2	0.005036216754857505
3	0.001613545173886385
4	0.003618252814169469
5	0.009143422652022849
6	0.012859466082791492
7	0.023176376134004438
8	0.035497993825500465
9	0.05045995816517422
10	0.05975006674209583
11	0.056718557627521406
12	0.06449291164715582
13	0.06659540990403806
14	0.06860011754432115
15	0.06175477438237891
16	0.06938244247711456
17	0.06512855065505045
18	0.07872144636233576
19	0.07564104193946175
20	0.08097063054411677
21	0.07564104193946175
22	0.07916150413703203
23	0.08453998804998665
24	0.08028609622792254
25	0.08101952585241635
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	2045186.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	48.809832951023054
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.57122944958518	37.3742891828871
2	13.585450261786027	13.262071156844165
3	4.279149808257863	6.265937619404058
4	1.7636088919619184	3.443258216304004
5	0.9584457157831403	2.33907876399994
6	0.5846953181272403	1.712332848302153
7	0.39351405259561384	1.3445148619750407
8	0.27894329177376925	1.0892140379428927
9	0.20649389778455413	0.9071039390642743
>10	1.176586546016148	10.462600036325362
>50	0.09683347533062091	3.3147103767863033
>100	0.0853290202106399	9.037429990461836
>500	0.013079771440526513	4.396366604387982
>1k	0.006640499346728844	5.051092365314911
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	4238	0.20721831657365147	No Hit
TATCAACGCAGAGTACTTTTTTTTT	3128	0.15294452436110945	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTG	2547	0.12453635023904916	No Hit
GAATAGGACCGCGGTTCTATTTTGT	2340	0.11441502142103457	No Hit
GATTAAGAGGGACGGCCGGGGGCAT	2317	0.11329042933014405	No Hit
GCGCAAGACGGACCAGAGCGAAAGC	2268	0.11089455922346426	No Hit
GTATCTGATCGTCTTCGAACCTCCG	2215	0.10830310788358613	No Hit
CTATTGGAGCTGGAATTACCGCGGC	2203	0.1077163641839911	No Hit
GTCTTGCGCCGGTCCAAGAATTTCA	2173	0.10624950493500346	No Hit
GTCCTATTCCATTATTCCTAGCTGC	2053	0.10038206793905298	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	9.779061659917485E-5	0.0	0.0	0.0	4.889530829958742E-5
7	9.779061659917485E-5	0.0	0.0	0.0	4.889530829958742E-5
8	9.779061659917485E-5	0.0	0.0	0.0	4.889530829958742E-5
9	9.779061659917485E-5	0.0	0.0	0.0	4.889530829958742E-5
10	9.779061659917485E-5	0.0	0.0	0.0	4.889530829958742E-5
11	9.779061659917485E-5	0.0	0.0	0.0	4.889530829958742E-5
12	9.779061659917485E-5	0.0	0.0	0.0	1.955812331983497E-4
13	9.779061659917485E-5	0.0	0.0	4.889530829958742E-5	2.4447654149793713E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGGGTAA	75	1.4763642E-5	12.668966	19
CGTTATT	165	0.0	12.66091	2
CGTCTTA	160	0.0	12.471319	15
CCGAGTA	55	0.003066529	12.092217	14
AAGACGG	475	0.0	11.999244	5
CGCATCG	330	0.0	11.80344	13
GTCCTAC	820	0.0	11.69905	1
CCGATAA	195	0.0	11.692429	9
GTCCTAA	490	0.0	11.630496	1
CCGTCGT	450	0.0	11.61123	9
ACCGTCG	455	0.0	11.483354	8
CGTCGTA	465	0.0	11.440977	10
CGAACGA	200	0.0	11.402349	16
CGCAAGA	470	0.0	11.314006	2
CTAGGAC	210	0.0	11.306321	3
TACCGTC	475	0.0	11.1998415	7
CAAGACG	510	0.0	11.175493	4
TAATATA	375	0.0	11.14569	4
GTTATTC	205	0.0	11.118801	3
ACGAACA	60	0.0058773495	11.083177	11
>>END_MODULE