FastQCFastQC Report
Thu 2 Feb 2017
SRR4063989_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4063989_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2045186
Sequences flagged as poor quality0
Sequence length50
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA24050.11759321646050776No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAA23970.11720205399411104No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT23520.11500176512062961No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATACGAATGCC22410.1095743858993754No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA21900.10708072517609646No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT21780.10649398147650141No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCG20740.10140886941334432No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA34200.020.6496851
TAGGACG9500.019.2192274
TTAGGAC7650.018.403483
TGTAGGA11250.018.1849382
GTCCTAC9200.018.1744081
GTCCTAA7150.018.154341
TGGCGAG12000.017.41501218
ACCGTCG5850.017.2975038
CGTCGTA6100.016.95003910
GTAGGAC10850.016.8278943
AGGACGT10900.016.5488875
TACCGTC5950.016.2673637
TATGGCG12750.016.21806716
TTTAGGA9650.016.1849922
CGGTCCA8200.016.09681710
ATACCGT6150.016.0960316
CTGTAGG12350.016.0328241
CTTTAGG9200.016.0221751
CGCAATA7450.015.94830236
TCCTACA11450.015.9460832
Produced by FastQC (version 0.11.5)