##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4063989_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2045186
Sequences flagged as poor quality	0
Sequence length	50
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.309884284363378	32.0	32.0	32.0	32.0	32.0
2	31.409801846873584	32.0	32.0	32.0	32.0	32.0
3	31.482906200218466	32.0	32.0	32.0	32.0	32.0
4	31.565648796735356	32.0	32.0	32.0	32.0	32.0
5	31.51422706785593	32.0	32.0	32.0	32.0	32.0
6	35.10798675523889	36.0	36.0	36.0	36.0	36.0
7	35.125718638793735	36.0	36.0	36.0	36.0	36.0
8	35.0702972736954	36.0	36.0	36.0	36.0	36.0
9	35.18214773619612	36.0	36.0	36.0	36.0	36.0
10	35.03025250515112	36.0	36.0	36.0	36.0	36.0
11	35.17442227748479	36.0	36.0	36.0	36.0	36.0
12	35.07763010308109	36.0	36.0	36.0	36.0	36.0
13	35.1210124653699	36.0	36.0	36.0	36.0	36.0
14	35.06562483803429	36.0	36.0	36.0	36.0	36.0
15	35.03734281380764	36.0	36.0	36.0	36.0	36.0
16	35.05114253666903	36.0	36.0	36.0	36.0	36.0
17	35.009583969379804	36.0	36.0	36.0	36.0	36.0
18	35.01130117260728	36.0	36.0	36.0	36.0	36.0
19	35.00558482211398	36.0	36.0	36.0	36.0	36.0
20	35.00054420478138	36.0	36.0	36.0	36.0	36.0
21	34.993154167884974	36.0	36.0	36.0	36.0	36.0
22	34.97696688711931	36.0	36.0	36.0	36.0	36.0
23	34.92112697818194	36.0	36.0	36.0	32.0	36.0
24	34.891999554074786	36.0	36.0	36.0	32.0	36.0
25	34.86097645886487	36.0	36.0	36.0	32.0	36.0
26	34.80645476743925	36.0	36.0	36.0	32.0	36.0
27	34.78998829446319	36.0	36.0	36.0	32.0	36.0
28	34.76708133147792	36.0	36.0	36.0	32.0	36.0
29	34.7338755497055	36.0	36.0	36.0	32.0	36.0
30	34.72408719793701	36.0	36.0	36.0	32.0	36.0
31	34.71394288832409	36.0	36.0	36.0	32.0	36.0
32	34.67155750137152	36.0	36.0	36.0	32.0	36.0
33	34.65289171742815	36.0	36.0	36.0	32.0	36.0
34	34.646334367632086	36.0	36.0	36.0	32.0	36.0
35	34.60254079580048	36.0	36.0	36.0	32.0	36.0
36	34.57687026999011	36.0	36.0	36.0	32.0	36.0
37	34.55919119336823	36.0	36.0	36.0	32.0	36.0
38	34.517591065066945	36.0	36.0	36.0	32.0	36.0
39	34.50029141603746	36.0	36.0	36.0	32.0	36.0
40	34.48593379770838	36.0	36.0	36.0	32.0	36.0
41	34.47475681918417	36.0	36.0	36.0	32.0	36.0
42	34.401625084466644	36.0	36.0	36.0	32.0	36.0
43	34.397086132997195	36.0	36.0	36.0	32.0	36.0
44	34.316708602542754	36.0	36.0	36.0	32.0	36.0
45	34.29513648147406	36.0	36.0	36.0	32.0	36.0
46	34.26219131169488	36.0	36.0	36.0	32.0	36.0
47	34.24706652597857	36.0	36.0	36.0	32.0	36.0
48	34.19890073567881	36.0	36.0	36.0	32.0	36.0
49	34.177227401321936	36.0	36.0	36.0	32.0	36.0
50	33.587689334857565	36.0	36.0	36.0	27.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	1.0
18	2.0
19	11.0
20	21.0
21	78.0
22	244.0
23	704.0
24	1755.0
25	4183.0
26	8051.0
27	14619.0
28	23853.0
29	36213.0
30	51658.0
31	72763.0
32	105292.0
33	170084.0
34	355245.0
35	1200409.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.77908938430017	17.928547327859796	11.621572721627	26.670790566213036
2	15.928833578299786	20.246061711987906	37.20742155450528	26.61768315520703
3	18.0505784084636	24.13664120681739	28.975626118177317	28.837154266541692
4	12.08457323685963	15.832887571106003	36.13245934599591	35.95007984603845
5	14.195774858619217	36.92358543428324	33.48570741243095	15.394932294666596
6	34.43253622299075	35.8624844084552	16.542708486641022	13.162270881913027
7	30.367683298911004	30.487965874953062	20.71657122293153	18.427779603204407
8	28.14394387600932	33.25056987481823	19.175908694857092	19.42957755431535
9	27.574827666800637	13.660358132298308	18.31836633309961	40.44644786780144
10	15.83526063847993	26.52366306957368	31.617078286890575	26.023998005055816
11	37.653005643496485	20.630250744920023	22.66801161361363	19.048731997969867
12	24.990697663047598	23.352508452780555	28.913179003366444	22.743614880805403
13	29.74755680815266	19.26883447023797	25.289743214877173	25.693865506732195
14	23.502967953920926	19.771707135802227	24.974329887833836	31.750995022443014
15	25.071451168963126	27.841580595207184	21.923443464705688	25.163524771123996
16	25.748190002879955	25.496132593443377	24.029505630097074	24.72617177357959
17	23.968186756607956	26.07850826281815	25.20054410699076	24.752760873583135
18	24.901658878424705	24.582762274365493	26.517411451429385	23.998167395780413
19	25.85932323970692	24.575918559934674	25.09826739390324	24.466490806455162
20	26.023713350971576	24.146174145318476	24.87990037048138	24.95021213322857
21	26.99187262185444	23.92510998999602	24.213445623038687	24.86957176511085
22	26.171020476417254	24.342821323481235	24.747333000192647	24.73882519990886
23	24.34253470991338	24.10460769668951	25.56238738683676	25.99047020656035
24	25.24666520958292	24.677326847843787	25.222657554514733	24.853350388058562
25	24.98566116338939	24.367660972693866	25.3681279322493	25.27854993166744
26	24.69992425676029	25.277264754651068	25.664733722660433	24.35807726592821
27	25.27001652763244	24.77824611502841	24.82998053846831	25.12175681887084
28	24.43939062029362	24.731406251833654	25.49185955084749	25.337343577025234
29	24.641281095287574	24.649984719760408	25.344318806918892	25.36441537803313
30	24.353665256961854	24.910163712545394	25.84082315416929	24.895347876323463
31	25.47032386369471	24.514835256453825	24.56456565121145	25.450275228640013
32	24.90412500837363	24.802761317111884	24.46282866797939	25.830285006535096
33	24.356296889952038	24.53506731518214	25.479430888067515	25.629204906798314
34	25.3194322269723	24.607148847031922	25.49027853228648	24.583140393709296
35	25.755589511643546	24.388875985575453	25.463724711203472	24.391809791577533
36	24.500822244161775	25.08702878661407	25.145512522414283	25.266636446809876
37	25.689050446017962	24.76083041537883	24.63814674805156	24.911972390551647
38	24.725443722747915	24.81331542321935	25.228811159950453	25.23242969408228
39	25.617485579941597	24.349459284483448	24.650818456892388	25.382236678682567
40	25.83970294279869	24.58418250593606	25.223800172898713	24.35231437836654
41	24.752874170216593	24.9907095552713	25.723138879435453	24.53327739507665
42	26.006385870893922	25.321493883059347	24.904456418631487	23.767663827415237
43	24.954196356929565	24.38020226458492	25.312215331641845	25.353386046843667
44	24.58515409819393	25.10359840344775	24.834572602494575	25.476674895863738
45	24.727148086919488	25.2679867609186	24.84552530685908	25.15933984530283
46	24.66653041027517	24.71747986958503	25.032613521458423	25.58337619868138
47	25.144257827035343	24.531727431553016	25.43504718298117	24.888967558430473
48	25.610361339389314	25.227111480333143	24.341451274019736	24.821075906257807
49	24.97701909273334	25.30652618649935	24.595682718748897	25.12077200201841
50	24.742562929061783	25.801746562616128	24.520526511373195	24.935163996948894
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	47.0
1	43.0
2	39.0
3	114.0
4	189.0
5	245.0
6	301.0
7	283.0
8	265.0
9	362.0
10	459.0
11	723.5
12	988.0
13	1799.5
14	2611.0
15	3412.5
16	4214.0
17	4572.0
18	4930.0
19	5004.0
20	5078.0
21	5694.5
22	6311.0
23	7111.5
24	7912.0
25	9370.0
26	10828.0
27	14060.5
28	17293.0
29	21038.0
30	24783.0
31	28785.0
32	32787.0
33	39098.0
34	45409.0
35	53666.0
36	61923.0
37	71122.0
38	80321.0
39	85334.0
40	90347.0
41	97491.5
42	104636.0
43	106944.0
44	109252.0
45	120258.5
46	131265.0
47	139859.5
48	148454.0
49	153870.5
50	159287.0
51	153174.5
52	147062.0
53	146097.5
54	145133.0
55	145976.0
56	146819.0
57	142872.5
58	138926.0
59	126869.5
60	114813.0
61	100546.0
62	86279.0
63	75367.0
64	64455.0
65	55288.5
66	46122.0
67	39853.5
68	33585.0
69	30876.0
70	28167.0
71	21556.0
72	14945.0
73	12879.0
74	10813.0
75	8536.5
76	6260.0
77	5442.0
78	4624.0
79	3829.0
80	3034.0
81	2448.5
82	1863.0
83	1541.0
84	1219.0
85	884.5
86	550.0
87	392.5
88	235.0
89	181.0
90	127.0
91	100.0
92	73.0
93	68.5
94	64.0
95	51.0
96	38.0
97	32.5
98	27.0
99	25.5
100	24.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.009583480426719134
2	3.9116246639669933E-4
3	2.4447654149793713E-4
4	0.0
5	0.0
6	3.4226715809711195E-4
7	9.779061659917483E-5
8	0.0
9	9.779061659917485E-4
10	7.823249327933987E-4
11	0.0
12	4.8895308299587416E-5
13	6.356390078946365E-4
14	2.9337184979752457E-4
15	0.0040583105888657555
16	8.312202410929862E-4
17	0.0
18	4.400577746962868E-4
19	4.8895308299587416E-5
20	4.400577746962868E-4
21	0.0
22	1.9558123319834967E-4
23	0.001026801474291336
24	2.4447654149793713E-4
25	0.002004707640283084
26	0.005818541687650903
27	0.006160808845748014
28	0.0044005777469628676
29	0.0029826138062748328
30	0.0037649387390682318
31	0.0077743540196344
32	0.0036671481224690564
33	0.005134007371456679
34	0.003569357505869882
35	0.0029826138062748328
36	0.008458888335828625
37	0.00435168243866328
38	0.0077743540196344
39	0.005769646379351316
40	0.0028359278813760705
41	0.003618252814169469
42	0.0022491841817810217
43	0.002102498256882259
44	0.0014179639406880352
45	0.0014179639406880352
46	0.0010756967825909234
47	0.003080404422874007
48	0.0022002888734814338
49	4.889530829958743E-4
50	0.001271278015789273
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	2045186.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	52.57496183627202
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.63367397134047	40.81587446252809
2	13.177943761146228	13.856597806456033
3	4.0587359792025905	6.4016366763024095
4	1.7414070253645422	3.6621763159982703
5	0.8790489305952429	2.310798198913031
6	0.5149826168124108	1.6245114855153595
7	0.36704374668422685	1.3508117681915863
8	0.25742299670716173	1.0827203382126243
9	0.1878396097511121	0.8888094282604453
>10	1.0152052407183227	9.657363314697509
>50	0.08022767792254484	2.9497858707862923
>100	0.07155893552387228	8.046884401205041
>500	0.010127213138292079	3.640543760083296
>1k	0.004782295093082369	3.711486172850034
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA	2405	0.11759321646050776	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAA	2397	0.11720205399411104	No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT	2352	0.11500176512062961	No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATACGAATGCC	2241	0.1095743858993754	No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA	2190	0.10708072517609646	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT	2178	0.10649398147650141	No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCG	2074	0.10140886941334432	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	4.889530829958742E-5
2	0.0	0.0	0.0	0.0	4.889530829958742E-5
3	0.0	0.0	0.0	0.0	4.889530829958742E-5
4	0.0	0.0	0.0	4.889530829958742E-5	4.889530829958742E-5
5	0.0	0.0	0.0	4.889530829958742E-5	4.889530829958742E-5
6	0.0	0.0	0.0	4.889530829958742E-5	9.779061659917485E-5
7	0.0	0.0	0.0	4.889530829958742E-5	9.779061659917485E-5
8	0.0	0.0	0.0	4.889530829958742E-5	9.779061659917485E-5
9	0.0	0.0	0.0	4.889530829958742E-5	9.779061659917485E-5
10	0.0	0.0	0.0	4.889530829958742E-5	9.779061659917485E-5
11	0.0	0.0	0.0	4.889530829958742E-5	9.779061659917485E-5
12	0.0	0.0	0.0	4.889530829958742E-5	1.955812331983497E-4
13	0.0	0.0	0.0	9.779061659917485E-5	2.4447654149793713E-4
14	0.0	0.0	0.0	9.779061659917485E-5	2.933718497975245E-4
15	0.0	0.0	0.0	1.4668592489876226E-4	3.911624663966994E-4
16	9.779061659917485E-5	0.0	0.0	1.955812331983497E-4	3.911624663966994E-4
17	9.779061659917485E-5	0.0	0.0	2.4447654149793713E-4	4.889530829958743E-4
18	9.779061659917485E-5	0.0	0.0	2.4447654149793713E-4	4.889530829958743E-4
19	9.779061659917485E-5	0.0	0.0	2.4447654149793713E-4	4.889530829958743E-4
20	9.779061659917485E-5	0.0	0.0	2.4447654149793713E-4	5.86743699595049E-4
21	9.779061659917485E-5	0.0	0.0	3.4226715809711195E-4	6.845343161942239E-4
22	9.779061659917485E-5	0.0	0.0	6.356390078946365E-4	7.334296244938113E-4
23	9.779061659917485E-5	0.0	0.0	0.0010756967825909232	7.334296244938113E-4
24	9.779061659917485E-5	0.0	0.0	0.00151575455728721	7.334296244938113E-4
25	9.779061659917485E-5	0.0	0.0	0.0022491841817810212	7.334296244938113E-4
26	9.779061659917485E-5	0.0	0.0	0.0025914513398781333	7.334296244938113E-4
27	9.779061659917485E-5	0.0	0.0	0.0029826138062748328	7.823249327933988E-4
28	9.779061659917485E-5	0.0	0.0	0.005720751071051728	8.312202410929862E-4
29	9.779061659917485E-5	0.0	0.0	0.010365805359512533	8.312202410929862E-4
30	9.779061659917485E-5	0.0	0.0	0.019362542086636618	8.312202410929862E-4
31	9.779061659917485E-5	0.0	0.0	0.04366351031153157	8.312202410929862E-4
32	9.779061659917485E-5	0.0	0.0	0.06830674569452362	9.29010857692161E-4
33	9.779061659917485E-5	0.0	0.0	0.09153201713682765	9.29010857692161E-4
34	9.779061659917485E-5	0.0	0.0	0.11832664608500156	9.29010857692161E-4
35	9.779061659917485E-5	0.0	0.0	0.15010859647973337	9.29010857692161E-4
36	9.779061659917485E-5	0.0	0.0	0.19269641000867402	9.29010857692161E-4
37	9.779061659917485E-5	0.0	0.0	0.2554290905570447	9.29010857692161E-4
38	9.779061659917485E-5	0.0	0.0	0.3436851220378	9.779061659917485E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATCAA	3420	0.0	20.649685	1
TAGGACG	950	0.0	19.219227	4
TTAGGAC	765	0.0	18.40348	3
TGTAGGA	1125	0.0	18.184938	2
GTCCTAC	920	0.0	18.174408	1
GTCCTAA	715	0.0	18.15434	1
TGGCGAG	1200	0.0	17.415012	18
ACCGTCG	585	0.0	17.297503	8
CGTCGTA	610	0.0	16.950039	10
GTAGGAC	1085	0.0	16.827894	3
AGGACGT	1090	0.0	16.548887	5
TACCGTC	595	0.0	16.267363	7
TATGGCG	1275	0.0	16.218067	16
TTTAGGA	965	0.0	16.184992	2
CGGTCCA	820	0.0	16.096817	10
ATACCGT	615	0.0	16.096031	6
CTGTAGG	1235	0.0	16.032824	1
CTTTAGG	920	0.0	16.022175	1
CGCAATA	745	0.0	15.948302	36
TCCTACA	1145	0.0	15.946083	2
>>END_MODULE