FastQCFastQC Report
Thu 2 Feb 2017
SRR4063988_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4063988_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1042173
Sequences flagged as poor quality0
Sequence length25
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT34050.32672118736524547No Hit
TATCAACGCAGAGTACTTTTTTTTT24140.23163140860490533No Hit
GTACTTTTTTTTTTTTTTTTTTTTT17180.1648478707469873No Hit
GTCCTAAAGTGTGTATTTCTCATTT13350.12809773425333412No Hit
GTACATGGGGTGGTATCAACGCAAA12580.120709325611007No Hit
GGTATCAACGCAGAGTACTTTTTTT11920.11437640391758375No Hit
ACGCAGAGTACTTTTTTTTTTTTTT11670.1119775699428022No Hit
GATTAAGAGGGACGGCCGGGGGCAT11620.1114978031478459No Hit
GAGTACTTTTTTTTTTTTTTTTTTT11500.11034636283995077No Hit
GTCCTACAGTGTGCATTTCTCATTT11260.10804348222416048No Hit
GTTCAAAGCAGGCCCGAGCCGCCTG10990.10545274153139642No Hit
CTTTAGGACGTGAAATATGGCGAGG10930.10487702137744884No Hit
GAATAGGACCGCGGTTCTATTTTGT10830.10391748778753623No Hit
GCGCAAGACGGACCAGAGCGAAAGC10680.10247818740266731No Hit
GTATCTGATCGTCTTCGAACCTCCG10650.10219032732569353No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACGGA350.002171165516.2839031
GTCATAA653.373434E-614.6137591
CTAGTAT400.00529492814.2429433
CGAACGA903.6210622E-813.7259716
CGACCAT1400.013.57317710
CAATTAA500.001500224313.2985211
TAAATTG500.001502941113.2953295
GCGTTAT751.4791645E-512.6652571
TAGACAG751.4828205E-512.6622175
CTAACAC701.0952871E-412.2082373
CTTATAC701.0952871E-412.2082373
GAATTTC2200.012.0936318
GTGTTAC550.003069832212.0895651
CGGTAAT550.003069832212.0895651
TCTAGTA550.003077569912.0855022
CGTCGTA1900.012.00154710
CCGTCGT1750.011.9438239
ACCGTCG1750.011.9426778
AGGACGT6000.011.8708295
GTTATTC1201.0068106E-811.869123
Produced by FastQC (version 0.11.5)