##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4063988_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1042173
Sequences flagged as poor quality	0
Sequence length	25
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.216191553609622	32.0	32.0	32.0	32.0	32.0
2	30.794378668416854	32.0	32.0	32.0	32.0	32.0
3	30.82334698749632	32.0	32.0	32.0	32.0	32.0
4	30.850510423893155	32.0	32.0	32.0	32.0	32.0
5	30.762327367913006	32.0	32.0	32.0	32.0	32.0
6	34.412717466293984	36.0	36.0	36.0	32.0	36.0
7	34.340209351038645	36.0	36.0	36.0	32.0	36.0
8	34.285751981676746	36.0	36.0	36.0	32.0	36.0
9	34.4329962491832	36.0	36.0	36.0	32.0	36.0
10	34.105822161963516	36.0	36.0	36.0	32.0	36.0
11	34.41952439758082	36.0	36.0	36.0	32.0	36.0
12	34.20457160183578	36.0	36.0	36.0	32.0	36.0
13	34.31133314718382	36.0	36.0	36.0	32.0	36.0
14	34.20085916637641	36.0	36.0	36.0	32.0	36.0
15	34.140105337597504	36.0	36.0	36.0	32.0	36.0
16	34.13956895832074	36.0	36.0	36.0	32.0	36.0
17	34.055373723940264	36.0	36.0	36.0	32.0	36.0
18	34.05313705114218	36.0	36.0	36.0	32.0	36.0
19	34.06622988697654	36.0	36.0	36.0	32.0	36.0
20	34.03554016463677	36.0	36.0	36.0	32.0	36.0
21	34.01913022118209	36.0	36.0	36.0	32.0	36.0
22	33.9932554383965	36.0	36.0	36.0	32.0	36.0
23	33.94282139337711	36.0	36.0	36.0	32.0	36.0
24	33.925294552823765	36.0	36.0	36.0	32.0	36.0
25	33.53442950450645	36.0	36.0	36.0	27.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
3	3.0
4	14.0
5	16.0
6	47.0
7	24.0
8	67.0
9	60.0
10	83.0
11	27.0
12	55.0
13	38.0
14	93.0
15	191.0
16	302.0
17	422.0
18	537.0
19	727.0
20	1075.0
21	1728.0
22	2715.0
23	4178.0
24	5913.0
25	8697.0
26	12344.0
27	16148.0
28	21953.0
29	29556.0
30	38017.0
31	52238.0
32	72766.0
33	102251.0
34	216852.0
35	453036.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.536785640919305	17.30127920839981	11.307353672420513	25.854581478260368
2	16.440158330334654	19.838023269761305	38.31594098596617	25.405877413937866
3	18.998357239086964	24.213451448354746	28.403547288868758	28.384644023689532
4	12.352356962936307	15.56569509415857	36.154204150173925	35.9277437927312
5	14.299437077640295	36.95797370981765	33.621128109459896	15.12146110308216
6	33.60079461046813	35.93021247192952	17.12270397881039	13.346288938791961
7	29.976005835380835	30.494087837837835	20.889319717444717	18.64058660933661
8	27.80838695805753	33.57106107843288	19.422868705174082	19.19768325833551
9	27.911734296655478	13.749420403523946	18.504175494612994	39.83466980520758
10	16.111044973290987	26.691730767015237	31.500408987745725	25.696815271948047
11	37.224142847130956	20.714427663911014	23.168976696808905	18.892452792149133
12	25.432034756726914	23.522911115911764	29.12412088043974	21.92093324692158
13	30.147763386556313	19.644177316063868	25.122464067285627	25.08559523009419
14	23.53511621230553	20.27183895519823	25.160273797670474	31.032771034825767
15	24.757096992227105	28.469768656487144	22.19826261943531	24.57487173185044
16	24.955448358502448	25.45963083588097	25.222278125144026	24.362642680472554
17	23.530671203507858	26.068722774872377	25.72135486924738	24.679251152372384
18	24.17360927063288	24.74764642844797	27.61543159374532	23.46331270717383
19	25.75687304774333	24.31067875397413	25.594691110144485	24.337757088138055
20	26.145728305437927	24.231171799229877	25.33392869146045	24.28917120387175
21	26.91941325815335	23.828417571527886	24.401869769121156	24.850299401197603
22	26.061643145016088	24.83231401379514	24.968959487449837	24.13708335373893
23	24.114658856292312	24.716569837230516	25.928170163729774	25.240601142747394
24	25.048469106046273	24.860642317682704	25.91865640469183	24.172232171579193
25	24.62688573705791	24.773999635096075	25.99066614171716	24.60844848612885
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	132.0
1	132.0
2	343.5
3	555.0
4	555.0
5	555.0
6	1398.5
7	2242.0
8	2242.0
9	2242.0
10	2192.5
11	2143.0
12	2143.0
13	2143.0
14	2180.0
15	2217.0
16	2217.0
17	2217.0
18	4022.5
19	5828.0
20	5828.0
21	5828.0
22	10342.0
23	14856.0
24	14856.0
25	14856.0
26	23662.0
27	32468.0
28	32468.0
29	32468.0
30	42416.0
31	52364.0
32	52364.0
33	52364.0
34	61551.5
35	70739.0
36	70739.0
37	70739.0
38	79687.0
39	88635.0
40	88635.0
41	88635.0
42	99928.0
43	111221.0
44	111221.0
45	111221.0
46	122377.0
47	133533.0
48	133533.0
49	133533.0
50	136116.5
51	138700.0
52	138700.0
53	138700.0
54	126484.5
55	114269.0
56	114269.0
57	114269.0
58	105581.0
59	96893.0
60	96893.0
61	96893.0
62	85747.5
63	74602.0
64	74602.0
65	74602.0
66	61618.0
67	48634.0
68	48634.0
69	48634.0
70	36446.0
71	24258.0
72	24258.0
73	24258.0
74	18634.5
75	13011.0
76	13011.0
77	13011.0
78	10623.0
79	8235.0
80	8235.0
81	8235.0
82	5696.5
83	3158.0
84	3158.0
85	3158.0
86	2464.5
87	1771.0
88	1771.0
89	1771.0
90	1267.0
91	763.0
92	763.0
93	763.0
94	538.5
95	314.0
96	314.0
97	314.0
98	473.0
99	632.0
100	632.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.04173971116119876
2	0.004605761231580553
3	0.0023988339747815384
4	0.004605761231580553
5	0.010650822848030029
6	0.014680863925663014
7	0.024276199824789166
8	0.035022976031810454
9	0.04797667949563077
10	0.05536508813795791
11	0.05335006759914141
12	0.06217777662633747
13	0.06169800983138117
14	0.06467256396011027
15	0.05805178218971323
16	0.06572805090901415
17	0.06006680272852971
18	0.0745557599362102
19	0.07196501924344614
20	0.07513148009015778
21	0.07302050619235002
22	0.07599506032107914
23	0.07896961444980824
24	0.07561124688511409
25	0.077050547269983
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	1042173.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	50.741293195914935
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.90899305614052	38.51720472868298
2	14.123341220216313	14.33273195521894
3	4.5100829588290345	6.865423252555308
4	1.9277490044030245	3.9126590976218796
5	0.9332339888325174	2.367674972387199
6	0.5716167494484233	1.740274384767498
7	0.3669964897020444	1.3035313540090108
8	0.24745649061617644	1.0045009874870066
9	0.19713042770435513	0.9002387546984517
>10	1.002876244791015	9.319661287507515
>50	0.09832635256456755	3.530478856022411
>100	0.0994559998199948	10.565090335811469
>500	0.00931805715931614	3.286899805870374
>1k	0.00342295977281001	2.3536302273599743
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	3405	0.32672118736524547	No Hit
TATCAACGCAGAGTACTTTTTTTTT	2414	0.23163140860490533	No Hit
GTACTTTTTTTTTTTTTTTTTTTTT	1718	0.1648478707469873	No Hit
GTCCTAAAGTGTGTATTTCTCATTT	1335	0.12809773425333412	No Hit
GTACATGGGGTGGTATCAACGCAAA	1258	0.120709325611007	No Hit
GGTATCAACGCAGAGTACTTTTTTT	1192	0.11437640391758375	No Hit
ACGCAGAGTACTTTTTTTTTTTTTT	1167	0.1119775699428022	No Hit
GATTAAGAGGGACGGCCGGGGGCAT	1162	0.1114978031478459	No Hit
GAGTACTTTTTTTTTTTTTTTTTTT	1150	0.11034636283995077	No Hit
GTCCTACAGTGTGCATTTCTCATTT	1126	0.10804348222416048	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTG	1099	0.10545274153139642	No Hit
CTTTAGGACGTGAAATATGGCGAGG	1093	0.10487702137744884	No Hit
GAATAGGACCGCGGTTCTATTTTGT	1083	0.10391748778753623	No Hit
GCGCAAGACGGACCAGAGCGAAAGC	1068	0.10247818740266731	No Hit
GTATCTGATCGTCTTCGAACCTCCG	1065	0.10219032732569353	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	9.595335899126153E-5	0.0
8	0.0	0.0	0.0	9.595335899126153E-5	0.0
9	0.0	0.0	0.0	9.595335899126153E-5	0.0
10	0.0	0.0	0.0	9.595335899126153E-5	0.0
11	0.0	0.0	0.0	9.595335899126153E-5	0.0
12	0.0	0.0	0.0	9.595335899126153E-5	0.0
13	0.0	0.0	0.0	9.595335899126153E-5	9.595335899126153E-5
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTACGGA	35	0.0021711655	16.283903	1
GTCATAA	65	3.373434E-6	14.613759	1
CTAGTAT	40	0.005294928	14.242943	3
CGAACGA	90	3.6210622E-8	13.72597	16
CGACCAT	140	0.0	13.573177	10
CAATTAA	50	0.0015002243	13.298521	1
TAAATTG	50	0.0015029411	13.295329	5
GCGTTAT	75	1.4791645E-5	12.665257	1
TAGACAG	75	1.4828205E-5	12.662217	5
CTAACAC	70	1.0952871E-4	12.208237	3
CTTATAC	70	1.0952871E-4	12.208237	3
GAATTTC	220	0.0	12.09363	18
GTGTTAC	55	0.0030698322	12.089565	1
CGGTAAT	55	0.0030698322	12.089565	1
TCTAGTA	55	0.0030775699	12.085502	2
CGTCGTA	190	0.0	12.001547	10
CCGTCGT	175	0.0	11.943823	9
ACCGTCG	175	0.0	11.942677	8
AGGACGT	600	0.0	11.870829	5
GTTATTC	120	1.0068106E-8	11.86912	3
>>END_MODULE