FastQCFastQC Report
Thu 2 Feb 2017
SRR4063988_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4063988_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1042173
Sequences flagged as poor quality0
Sequence length50
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA11660.11188161658381095No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAA11220.10765966878819543No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCG11060.10612441504433524No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT11010.10564464824937894No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTACGGC300.005747746729.3301014
CGATTAA602.871752E-422.00074441
ACCGTCG3750.021.1186898
CGTCGTA3750.021.11767610
TACCGTC3900.020.8704997
ATACCGT4050.020.6397026
CCGTCGT3900.020.306439
CGTGCAA654.9336033E-420.3064299
GTATCAA25700.020.2895981
TGTAGGA11000.019.9977972
AGGCGTG1352.5993359E-919.554347
GTCGTAG4050.019.55340211
GTCCTAC10100.019.3877241
GTGCGCC1152.02308E-719.12832811
ATTTCGT4850.019.04944842
TCCTACA10300.019.0076142
GTAGGAC11000.018.9979083
GATTTCG5100.018.98103341
TGCGAGG708.1152125E-418.8595925
AGTGTGC7700.018.855978
Produced by FastQC (version 0.11.5)