FastQCFastQC Report
Thu 2 Feb 2017
SRR4063987_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4063987_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2102747
Sequences flagged as poor quality0
Sequence length25
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT43890.20872696525069348No Hit
TATCAACGCAGAGTACTTTTTTTTT30230.14376432352536944No Hit
GTTCAAAGCAGGCCCGAGCCGCCTG22070.10495794310965609No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTATT1301.8189894E-1213.8810862
GCATCGC3500.012.2155414
CGTCTTA1103.8107828E-812.09215115
GCGTTAT1459.822543E-1111.792071
AAACCGA658.029788E-411.6915616
GGTATCA13100.011.6020361
TTGCGCG2800.011.536918
ATCGCCA3900.011.44989316
CGCATCG3750.011.400913
TTTGCGC2750.011.40062817
ACTCTAA3700.011.29765110
GTAATAC1604.5474735E-1111.2781143
ATTTGCG2800.011.19757916
TCGCGTA1451.2169039E-911.1385459
CGTCAAT2250.010.97864419
CGAACGA1658.0035534E-1110.94051716
CCTATTC4100.010.8872173
GTACTAA1053.4711466E-610.8561921
TACGCTG700.001493168110.8559345
GTATCAA29650.010.8287311
Produced by FastQC (version 0.11.5)