##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4063987_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2102747
Sequences flagged as poor quality	0
Sequence length	25
%GC	51
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.213511658796804	32.0	32.0	32.0	32.0	32.0
2	30.704231655068348	32.0	32.0	32.0	32.0	32.0
3	30.72956375636251	32.0	32.0	32.0	32.0	32.0
4	30.772936544434494	32.0	32.0	32.0	32.0	32.0
5	30.61922998820115	32.0	32.0	32.0	32.0	32.0
6	34.30094300455547	36.0	36.0	36.0	32.0	36.0
7	34.227721166645345	36.0	36.0	36.0	32.0	36.0
8	34.17047034189087	36.0	36.0	36.0	32.0	36.0
9	34.374020745244195	36.0	36.0	36.0	32.0	36.0
10	33.969242614541834	36.0	36.0	36.0	32.0	36.0
11	34.339827378186726	36.0	36.0	36.0	32.0	36.0
12	34.110396067620115	36.0	36.0	36.0	32.0	36.0
13	34.21973161773623	36.0	36.0	36.0	32.0	36.0
14	34.10588530146518	36.0	36.0	36.0	32.0	36.0
15	34.01499847580332	36.0	36.0	36.0	32.0	36.0
16	34.01645847075278	36.0	36.0	36.0	32.0	36.0
17	33.92851113329373	36.0	36.0	36.0	32.0	36.0
18	33.928225792261266	36.0	36.0	36.0	32.0	36.0
19	33.9519980292446	36.0	36.0	36.0	32.0	36.0
20	33.93255251345026	36.0	36.0	36.0	32.0	36.0
21	33.92430425533838	36.0	36.0	36.0	32.0	36.0
22	33.89201363739908	36.0	36.0	36.0	32.0	36.0
23	33.82966614623633	36.0	36.0	36.0	32.0	36.0
24	33.8096100006325	36.0	36.0	36.0	32.0	36.0
25	33.38037196105856	36.0	36.0	36.0	21.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
3	3.0
4	15.0
5	29.0
6	81.0
7	43.0
8	118.0
9	166.0
10	178.0
11	68.0
12	148.0
13	106.0
14	156.0
15	378.0
16	635.0
17	788.0
18	1090.0
19	1560.0
20	2383.0
21	3586.0
22	5676.0
23	8721.0
24	12946.0
25	18757.0
26	26805.0
27	35004.0
28	46779.0
29	63038.0
30	82981.0
31	112618.0
32	156667.0
33	219006.0
34	449456.0
35	852762.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.806629637118355	16.936332171699753	11.27742426585154	25.97961392533035
2	17.130680561639203	19.448158045800714	36.74933928016851	26.67182211239157
3	18.905007709069878	23.24558024423627	28.414747008395803	29.434665038298053
4	12.847433428656627	15.175046571761888	35.30726899954296	36.670251000038526
5	15.009314830818226	36.15471798440093	33.28478935926235	15.551177825518497
6	34.878647149249645	34.84164294846428	16.638953152111046	13.640756750175031
7	30.95098449140042	30.152317537422945	20.341271959179032	18.555426011997607
8	28.8112443035608	32.27351852142584	19.05102401507918	19.864213159934177
9	27.579138693146945	14.077147438489975	18.268250788651038	40.075463079712044
10	16.206820087385285	26.31670360856874	31.113615145006086	26.362861159039884
11	37.44752085247596	20.923994634433935	22.109405966741676	19.51907854634843
12	24.985057495331656	23.35894180417895	28.31823537585634	23.337765324633054
13	29.646535909143907	19.14069322267405	25.074369035070394	26.13840183311165
14	23.949115333786068	19.245077216448287	24.777887603917765	32.02791984584788
15	25.458714832338714	26.719118863994662	22.023784314686537	25.79838198898009
16	26.18064333140438	25.5402815003198	23.091335318125054	25.187739850150763
17	24.519015255413247	25.612467391066414	24.683142525666003	25.185374827854336
18	25.293912427637405	24.63862133760675	25.572154126672793	24.495312108083052
19	25.91994859957642	24.683911191490374	25.023344359041477	24.372795849891727
20	26.183079707747936	23.940881353867066	24.54781222647774	25.32822671190726
21	27.17434293658314	23.864931662929788	24.099043894972656	24.861681505514415
22	26.325315970710093	23.80526066542444	24.51004609567053	25.359377268194937
23	24.91182660994377	23.82818635547132	25.05204728854331	26.207939746041596
24	25.194864533631595	24.560760563045108	24.744380852658757	25.499994050664544
25	25.246924862116167	24.13547741027794	24.821374516668858	25.796223210937043
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	118.0
1	118.0
2	353.5
3	589.0
4	589.0
5	589.0
6	1587.5
7	2586.0
8	2586.0
9	2586.0
10	2789.0
11	2992.0
12	2992.0
13	2992.0
14	3913.0
15	4834.0
16	4834.0
17	4834.0
18	8146.0
19	11458.0
20	11458.0
21	11458.0
22	18788.0
23	26118.0
24	26118.0
25	26118.0
26	39266.0
27	52414.0
28	52414.0
29	52414.0
30	65868.0
31	79322.0
32	79322.0
33	79322.0
34	100902.5
35	122483.0
36	122483.0
37	122483.0
38	143689.5
39	164896.0
40	164896.0
41	164896.0
42	191307.5
43	217719.0
44	217719.0
45	217719.0
46	244270.5
47	270822.0
48	270822.0
49	270822.0
50	279627.5
51	288433.0
52	288433.0
53	288433.0
54	271398.5
55	254364.0
56	254364.0
57	254364.0
58	234305.0
59	214246.0
60	214246.0
61	214246.0
62	188745.5
63	163245.0
64	163245.0
65	163245.0
66	135270.0
67	107295.0
68	107295.0
69	107295.0
70	81636.0
71	55977.0
72	55977.0
73	55977.0
74	43067.0
75	30157.0
76	30157.0
77	30157.0
78	24221.5
79	18286.0
80	18286.0
81	18286.0
82	12637.0
83	6988.0
84	6988.0
85	6988.0
86	5387.0
87	3786.0
88	3786.0
89	3786.0
90	2730.0
91	1674.0
92	1674.0
93	1674.0
94	1193.0
95	712.0
96	712.0
97	712.0
98	972.5
99	1233.0
100	1233.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.039091721448181835
2	0.005516593294390623
3	0.0013791483235976558
4	0.0037094334220902467
5	0.008845572006522896
6	0.013458585364763333
7	0.023493078339904896
8	0.03690410686592348
9	0.05026757855319732
10	0.05939849159218869
11	0.05697309281620661
12	0.06462974385411084
13	0.06667468792013495
14	0.0687671888249038
15	0.06077763991578635
16	0.06824406359871159
17	0.06320303869176844
18	0.0806563985110905
19	0.07594827147536055
20	0.08008571644615353
21	0.07694696508900024
22	0.07999060276866404
23	0.08431827509443598
24	0.07951503438121657
25	0.08098929638230372
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	2102747.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	53.50466710461761
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.52351807770941	42.013746946312644
2	12.633945390499541	13.519500846731919
3	3.69449129564447	5.930175806830942
4	1.6212816067922358	3.4698453061703245
5	0.872276028234829	2.3335419257021277
6	0.555440383840381	1.7831191700304365
7	0.373074496186789	1.3972858706587954
8	0.2822713826856703	1.2082269091005529
9	0.19236396788322674	0.926313305806386
>10	1.0983086532017734	10.51303854320742
>50	0.07528182861783411	2.7709010894208843
>100	0.06434470932759857	7.4154639030652785
>500	0.009292177700898909	3.377060335472893
>1k	0.0041100016753975906	3.3417800414894394
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	4389	0.20872696525069348	No Hit
TATCAACGCAGAGTACTTTTTTTTT	3023	0.14376432352536944	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTG	2207	0.10495794310965609	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.9022735497898702E-4
2	0.0	0.0	0.0	0.0	1.9022735497898702E-4
3	0.0	0.0	0.0	0.0	1.9022735497898702E-4
4	0.0	0.0	0.0	0.0	1.9022735497898702E-4
5	0.0	0.0	0.0	0.0	2.3778419372373378E-4
6	0.0	0.0	0.0	0.0	2.3778419372373378E-4
7	0.0	0.0	0.0	0.0	2.3778419372373378E-4
8	0.0	0.0	0.0	0.0	2.3778419372373378E-4
9	0.0	0.0	0.0	0.0	2.8534103246848053E-4
10	0.0	0.0	0.0	0.0	3.328978712132273E-4
11	0.0	0.0	0.0	0.0	3.328978712132273E-4
12	0.0	0.0	0.0	0.0	5.706820649369611E-4
13	0.0	0.0	0.0	4.7556838744746755E-5	6.182389036817078E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTTATT	130	1.8189894E-12	13.881086	2
GCATCGC	350	0.0	12.21554	14
CGTCTTA	110	3.8107828E-8	12.092151	15
GCGTTAT	145	9.822543E-11	11.79207	1
AAACCGA	65	8.029788E-4	11.691561	6
GGTATCA	1310	0.0	11.602036	1
TTGCGCG	280	0.0	11.5369	18
ATCGCCA	390	0.0	11.449893	16
CGCATCG	375	0.0	11.4009	13
TTTGCGC	275	0.0	11.400628	17
ACTCTAA	370	0.0	11.297651	10
GTAATAC	160	4.5474735E-11	11.278114	3
ATTTGCG	280	0.0	11.197579	16
TCGCGTA	145	1.2169039E-9	11.138545	9
CGTCAAT	225	0.0	10.978644	19
CGAACGA	165	8.0035534E-11	10.940517	16
CCTATTC	410	0.0	10.887217	3
GTACTAA	105	3.4711466E-6	10.856192	1
TACGCTG	70	0.0014931681	10.855934	5
GTATCAA	2965	0.0	10.828731	1
>>END_MODULE