FastQCFastQC Report
Thu 2 Feb 2017
SRR4063987_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4063987_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2102747
Sequences flagged as poor quality0
Sequence length50
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAA21670.10305566955986623No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA34350.022.8010251
GGTATCA16800.018.7265551
ATACCGT6000.017.9657346
ACCGTCG5900.017.5245158
CGTCGTA6050.016.72799710
GTATTAC1602.131128E-816.500321
TACCGTC6400.016.4991447
CGTTTAT6950.016.46231831
CGCAATA7000.016.34395236
CCGTCGT6350.016.2837819
AACGCAG46950.016.212156
TCGTTTA7200.015.8907130
TATACCG1259.45148E-615.8391785
ATCGTTT7350.015.56529829
CATCGTT7600.015.34276928
GACGGTA6750.015.3177238
TCAACGC49850.015.2690174
CGCATCG7700.015.14315213
AATAACG7850.015.1326132
TATCAAC51500.015.1211862
Produced by FastQC (version 0.11.5)