##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4063987_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2102747
Sequences flagged as poor quality	0
Sequence length	50
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.330872901019475	32.0	32.0	32.0	32.0	32.0
2	31.398648054188165	32.0	32.0	32.0	32.0	32.0
3	31.474797015523027	32.0	32.0	32.0	32.0	32.0
4	31.55547148563284	32.0	32.0	32.0	32.0	32.0
5	31.504075858864617	32.0	32.0	32.0	32.0	32.0
6	35.08926846643938	36.0	36.0	36.0	36.0	36.0
7	35.10458604863067	36.0	36.0	36.0	36.0	36.0
8	35.0496688379534	36.0	36.0	36.0	36.0	36.0
9	35.16152918063847	36.0	36.0	36.0	36.0	36.0
10	35.012564041227975	36.0	36.0	36.0	36.0	36.0
11	35.158560207195634	36.0	36.0	36.0	36.0	36.0
12	35.06250775770932	36.0	36.0	36.0	36.0	36.0
13	35.10748986920443	36.0	36.0	36.0	36.0	36.0
14	35.05066990940898	36.0	36.0	36.0	36.0	36.0
15	35.017396291612826	36.0	36.0	36.0	36.0	36.0
16	35.02986902371041	36.0	36.0	36.0	36.0	36.0
17	34.99164473900093	36.0	36.0	36.0	36.0	36.0
18	34.99245796094347	36.0	36.0	36.0	36.0	36.0
19	34.986971328457486	36.0	36.0	36.0	36.0	36.0
20	34.984581597310566	36.0	36.0	36.0	36.0	36.0
21	34.97975220033604	36.0	36.0	36.0	36.0	36.0
22	34.966691665711565	36.0	36.0	36.0	36.0	36.0
23	34.910872777371694	36.0	36.0	36.0	32.0	36.0
24	34.87862686286082	36.0	36.0	36.0	32.0	36.0
25	34.859616967709385	36.0	36.0	36.0	32.0	36.0
26	34.79392718191965	36.0	36.0	36.0	32.0	36.0
27	34.794354717899964	36.0	36.0	36.0	32.0	36.0
28	34.77099432314016	36.0	36.0	36.0	32.0	36.0
29	34.73750717513805	36.0	36.0	36.0	32.0	36.0
30	34.72032108475247	36.0	36.0	36.0	32.0	36.0
31	34.72274695909684	36.0	36.0	36.0	32.0	36.0
32	34.679680437066374	36.0	36.0	36.0	32.0	36.0
33	34.66107049492878	36.0	36.0	36.0	32.0	36.0
34	34.64636306697857	36.0	36.0	36.0	32.0	36.0
35	34.60530296797475	36.0	36.0	36.0	32.0	36.0
36	34.58543942756784	36.0	36.0	36.0	32.0	36.0
37	34.58117928595309	36.0	36.0	36.0	32.0	36.0
38	34.543812926614564	36.0	36.0	36.0	32.0	36.0
39	34.54598104289294	36.0	36.0	36.0	32.0	36.0
40	34.5178597330064	36.0	36.0	36.0	32.0	36.0
41	34.50576317550328	36.0	36.0	36.0	32.0	36.0
42	34.43883833861135	36.0	36.0	36.0	32.0	36.0
43	34.42523755829874	36.0	36.0	36.0	32.0	36.0
44	34.36451651102106	36.0	36.0	36.0	32.0	36.0
45	34.34150756129958	36.0	36.0	36.0	32.0	36.0
46	34.32764712064742	36.0	36.0	36.0	32.0	36.0
47	34.299255687916805	36.0	36.0	36.0	32.0	36.0
48	34.248805015534444	36.0	36.0	36.0	32.0	36.0
49	34.22676146963948	36.0	36.0	36.0	32.0	36.0
50	33.63082767446583	36.0	36.0	36.0	27.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
16	1.0
17	2.0
18	3.0
19	13.0
20	22.0
21	73.0
22	233.0
23	604.0
24	1759.0
25	3871.0
26	8018.0
27	14733.0
28	23957.0
29	36866.0
30	53017.0
31	75667.0
32	108776.0
33	176018.0
34	364575.0
35	1234539.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.69014462314655	17.802801146027143	11.878759688152773	26.62829454267353
2	16.29910673734901	20.152220363801344	36.299667909647454	27.24900498920219
3	17.940882037527135	23.794754000128403	28.8613217484764	29.403042213868062
4	12.419753779223083	15.780310232281867	35.383286719705225	36.416649268789826
5	14.603754041736833	36.51913425628476	33.081963736008184	15.79514796597023
6	34.92260815763805	35.22440471746164	16.352893675445525	13.500093449454784
7	30.79444688041256	30.29923728305749	20.354098878323867	18.55221695820608
8	28.71011110704236	32.47085835813819	18.89047992934956	19.92855060546989
9	27.640514094805553	13.830006301347062	18.10888252148997	40.42059708235742
10	15.991281797399282	26.46444355353786	31.243047718510297	26.301226930552563
11	37.72132358291321	20.79051830771843	22.068513235306007	19.419644874062357
12	24.83257606957759	23.443059694323516	28.27379055768029	23.4505736784186
13	29.667613687703724	19.109787543265103	25.07911129034866	26.143487478682516
14	23.939743554380797	19.093842052249773	24.8357623792888	32.13065201408064
15	25.469622561287064	26.803703524035903	21.877291755245533	25.8493821594315
16	26.195206997372942	25.52845536188159	23.09490700669415	25.18143063405132
17	24.543573023085052	25.676900586185873	24.627891338278612	25.15163505245046
18	25.360434480725146	24.66852773048499	25.579624680179197	24.39141310861067
19	25.88966998391627	24.584804821885285	25.060468549220875	24.46505664497757
20	26.18438661285736	23.97778320985916	24.512658282027115	25.325171895256364
21	27.169174418035073	23.87262946992672	24.180560000798952	24.77763611123925
22	26.255742507395112	23.834140217050134	24.655354442299096	25.254762833255658
23	24.964213053366066	23.685061553548213	25.14345690966281	26.20726848342291
24	25.218287446997522	24.474112725730144	24.806775185805893	25.500824641466437
25	25.28060204184027	24.026411618144387	25.005338346544058	25.687647993471284
26	25.222769263548784	24.804577518377037	25.137827780906143	24.834825437168035
27	25.75736029551997	24.36674801043851	24.441988225049094	25.433903468992426
28	25.06243291226291	24.213649682282938	25.355966284562818	25.36795112089133
29	25.055381756027312	24.437788382243976	25.11896733594017	25.38786252578854
30	25.001605112008285	24.588603847417815	25.265889776601703	25.1439012639722
31	25.55947983856009	24.350059783751185	24.410556944855315	25.679903432833406
32	25.337476006673427	24.39372264190067	24.325713807410395	25.94308754401551
33	24.937970866163077	24.011184029988954	24.95276178862678	26.098083315221192
34	25.692488927168277	24.11882231582812	25.007312135195793	25.181376621807804
35	26.132116345021135	23.99997717195746	24.95927810120147	24.908628381819938
36	25.02005883290172	24.65398215988566	24.68503946370267	25.64091954350995
37	25.93504135949637	24.36369914123104	24.61718745912917	25.08407204014342
38	25.170135970851092	24.187249682890045	25.07477707182071	25.567837274438155
39	25.692784822106717	24.20993257514255	24.47350636367571	25.62377623907503
40	25.799759736794798	24.359226782403905	24.93049392826536	24.910519552535938
41	24.995077677576944	24.754002775524025	25.094332623440906	25.156586923458125
42	26.077498675508657	24.695699897369757	24.664501824801896	24.56229960231969
43	25.44617443497888	23.886329492098053	24.914574484629114	25.752921588293958
44	25.123602281244413	24.421080965914495	24.612357755127665	25.842958997713428
45	25.1917639813289	24.679141015306403	24.649274866066015	25.47982013729868
46	25.08397468608472	24.394723588576113	24.866399044664593	25.654902680674574
47	25.32189657648712	24.246656907757384	25.091097420737395	25.340349095018105
48	25.95916010803258	24.77311303234557	24.281078746886738	24.986648112735107
49	25.18005113338051	24.904315139893928	24.359786487699836	25.555847239025724
50	25.156880360029426	24.997705349792675	24.448748928172456	25.396665362005443
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	11.0
1	19.5
2	28.0
3	106.5
4	185.0
5	233.5
6	282.0
7	285.0
8	288.0
9	362.0
10	436.0
11	684.5
12	933.0
13	1806.5
14	2680.0
15	3558.5
16	4437.0
17	4645.5
18	4854.0
19	4929.5
20	5005.0
21	5483.0
22	5961.0
23	6843.0
24	7725.0
25	9139.5
26	10554.0
27	12985.5
28	15417.0
29	18255.5
30	21094.0
31	24253.0
32	27412.0
33	31802.0
34	36192.0
35	42565.5
36	48939.0
37	59458.5
38	69978.0
39	78016.0
40	86054.0
41	95615.5
42	105177.0
43	109514.5
44	113852.0
45	125730.0
46	137608.0
47	146186.0
48	154764.0
49	160950.0
50	167136.0
51	163559.5
52	159983.0
53	158062.5
54	156142.0
55	156837.5
56	157533.0
57	153478.0
58	149423.0
59	136501.0
60	123579.0
61	109103.5
62	94628.0
63	82340.5
64	70053.0
65	59509.5
66	48966.0
67	42989.5
68	37013.0
69	33121.0
70	29229.0
71	22927.5
72	16626.0
73	14248.5
74	11871.0
75	9470.0
76	7069.0
77	6169.0
78	5269.0
79	4449.0
80	3629.0
81	2854.5
82	2080.0
83	1711.5
84	1343.0
85	985.5
86	628.0
87	467.0
88	306.0
89	219.0
90	132.0
91	114.0
92	96.0
93	72.0
94	48.0
95	50.0
96	52.0
97	38.0
98	24.0
99	23.5
100	23.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.00851267413530967
2	2.8534103246848053E-4
3	9.511367748949351E-5
4	0.0
5	0.0
6	2.8534103246848053E-4
7	4.7556838744746755E-5
8	0.0
9	0.0010462504523844284
10	9.511367748949351E-4
11	0.0
12	4.7556838744746755E-5
13	6.182389036817077E-4
14	4.7556838744746755E-5
15	0.004803240713219422
16	7.133525811712012E-4
17	4.7556838744746755E-5
18	3.328978712132273E-4
19	4.7556838744746755E-5
20	6.182389036817077E-4
21	0.0
22	3.328978712132273E-4
23	0.0011889209686186688
24	7.133525811712012E-4
25	0.001712046194810883
26	0.005849491165603851
27	0.007038412134222519
28	0.004280115487027208
29	0.003281421873387526
30	0.004280115487027208
31	0.0080371057478622
32	0.0037569902608349933
33	0.005041024906943156
34	0.0037094334220902467
35	0.003328978712132273
36	0.00865534465154391
37	0.004089888132048221
38	0.0077993215541384674
39	0.005231252261922142
40	0.002520512453471578
41	0.0038521039383244866
42	0.002520512453471578
43	0.0018071598723003765
44	0.0016644893560661365
45	0.0015218188398318962
46	0.0014267051623424024
47	0.002520512453471578
48	0.0022827282597478443
49	5.231252261922142E-4
50	0.0014267051623424024
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	2102747.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	56.36504382141493
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.34183649256865	44.72106090775169
2	12.335422744265289	13.905732870723828
3	3.5760886379156664	6.046991783561417
4	1.5193582569099326	3.4255477892462785
5	0.8062364459433865	2.27217763030104
6	0.5145177844658978	1.740049048099059
7	0.34385542264245783	1.3566998175831302
8	0.25126309841124816	1.1329964442123597
9	0.18183902635789212	0.9224428220195412
>10	0.9990994647967112	9.953299660928185
>50	0.06461319592223799	2.522659168673642
>100	0.0555747114520403	6.741151930816472
>500	0.00706166630519525	2.738310312848667
>1k	0.0032330520433423975	2.520879813234719
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAA	2167	0.10305566955986623	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	9.511367748949351E-5
2	0.0	0.0	0.0	0.0	9.511367748949351E-5
3	0.0	0.0	0.0	0.0	9.511367748949351E-5
4	0.0	0.0	0.0	0.0	9.511367748949351E-5
5	0.0	0.0	0.0	0.0	9.511367748949351E-5
6	0.0	0.0	0.0	0.0	9.511367748949351E-5
7	0.0	0.0	0.0	0.0	9.511367748949351E-5
8	0.0	0.0	0.0	0.0	9.511367748949351E-5
9	0.0	0.0	0.0	0.0	9.511367748949351E-5
10	0.0	0.0	0.0	0.0	9.511367748949351E-5
11	0.0	0.0	0.0	0.0	9.511367748949351E-5
12	0.0	0.0	0.0	0.0	1.9022735497898702E-4
13	0.0	0.0	0.0	4.7556838744746755E-5	1.9022735497898702E-4
14	0.0	0.0	0.0	4.7556838744746755E-5	2.3778419372373378E-4
15	0.0	0.0	0.0	9.511367748949351E-5	2.3778419372373378E-4
16	0.0	0.0	0.0	9.511367748949351E-5	2.8534103246848053E-4
17	0.0	0.0	0.0	1.4267051623424027E-4	2.8534103246848053E-4
18	0.0	0.0	0.0	1.4267051623424027E-4	2.8534103246848053E-4
19	0.0	0.0	0.0	1.9022735497898702E-4	2.8534103246848053E-4
20	0.0	0.0	0.0	1.9022735497898702E-4	3.328978712132273E-4
21	0.0	0.0	0.0	1.9022735497898702E-4	3.8045470995797404E-4
22	0.0	0.0	0.0	2.3778419372373378E-4	3.8045470995797404E-4
23	0.0	0.0	0.0	5.706820649369611E-4	5.231252261922143E-4
24	0.0	0.0	0.0	8.560230974054416E-4	5.231252261922143E-4
25	0.0	0.0	0.0	0.0012840346461081623	5.231252261922143E-4
26	0.0	0.0	0.0	0.0016644893560661363	5.231252261922143E-4
27	0.0	0.0	0.0	0.002235171421003097	5.231252261922143E-4
28	0.0	0.0	0.0	0.003852103938324487	5.231252261922143E-4
29	0.0	0.0	0.0	0.007704207876648974	5.231252261922143E-4
30	0.0	0.0	0.0	0.01726313246434307	5.231252261922143E-4
31	0.0	0.0	0.0	0.04023308557805575	5.231252261922143E-4
32	0.0	0.0	0.0	0.0652004259190478	5.706820649369611E-4
33	0.0	0.0	0.0	0.08912151580765541	6.182389036817078E-4
34	0.0	0.0	0.0	0.11508754976228715	7.609094199159481E-4
35	0.0	0.0	0.0	0.14714085907624647	7.609094199159481E-4
36	0.0	0.0	0.0	0.18813485407421815	7.609094199159481E-4
37	0.0	0.0	0.0	0.2507196538623049	7.609094199159481E-4
38	0.0	0.0	0.0	0.33632196360284905	8.560230974054416E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATCAA	3435	0.0	22.801025	1
GGTATCA	1680	0.0	18.726555	1
ATACCGT	600	0.0	17.965734	6
ACCGTCG	590	0.0	17.524515	8
CGTCGTA	605	0.0	16.727997	10
GTATTAC	160	2.131128E-8	16.50032	1
TACCGTC	640	0.0	16.499144	7
CGTTTAT	695	0.0	16.462318	31
CGCAATA	700	0.0	16.343952	36
CCGTCGT	635	0.0	16.283781	9
AACGCAG	4695	0.0	16.21215	6
TCGTTTA	720	0.0	15.89071	30
TATACCG	125	9.45148E-6	15.839178	5
ATCGTTT	735	0.0	15.565298	29
CATCGTT	760	0.0	15.342769	28
GACGGTA	675	0.0	15.317723	8
TCAACGC	4985	0.0	15.269017	4
CGCATCG	770	0.0	15.143152	13
AATAACG	785	0.0	15.132613	2
TATCAAC	5150	0.0	15.121186	2
>>END_MODULE