FastQCFastQC Report
Thu 2 Feb 2017
SRR4063986_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4063986_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences980123
Sequences flagged as poor quality0
Sequence length25
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT21850.22293120353261783No Hit
TATCAACGCAGAGTACTTTTTTTTT15510.15824544470438914No Hit
GTACTTTTTTTTTTTTTTTTTTTTT12610.12865732158106685No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AATCGTA402.7613936E-416.62374913
CGTTATT601.4697343E-615.8289092
GTCCTAA851.7156708E-814.5283191
GCGTTAT759.670384E-713.9322851
ACGAACG655.439273E-513.15621515
GTATAAT751.4784044E-512.6657141
TGGATAG604.0957468E-412.6644215
CCTATTG604.0992763E-412.6631273
AGCGTAT1301.9645086E-1012.4240468
CGAACGA701.08827524E-412.21648516
CGATAAC701.08983295E-412.21461410
GATAACG701.0903526E-412.21398911
TCCAATA1800.012.1354972
CCGGCGT550.003062917612.09308616
CGTAAAT550.003062917612.09308616
CTATGGT802.8690281E-511.8728944
ATCGTAA658.010098E-411.69381614
CGCGTAA658.016965E-411.69262210
TCGCGTA658.016965E-411.6926229
GTATCAA13600.011.6645641
Produced by FastQC (version 0.11.5)