##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4063986_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	980123
Sequences flagged as poor quality	0
Sequence length	25
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.260758088525623	32.0	32.0	32.0	32.0	32.0
2	30.859841060764822	32.0	32.0	32.0	32.0	32.0
3	30.885594971243407	32.0	32.0	32.0	32.0	32.0
4	30.912016144912425	32.0	32.0	32.0	32.0	32.0
5	30.810439097949953	32.0	32.0	32.0	32.0	32.0
6	34.47573620861871	36.0	36.0	36.0	32.0	36.0
7	34.41173709830297	36.0	36.0	36.0	32.0	36.0
8	34.371445216569754	36.0	36.0	36.0	32.0	36.0
9	34.51516799422113	36.0	36.0	36.0	32.0	36.0
10	34.197451748403005	36.0	36.0	36.0	32.0	36.0
11	34.49702639362611	36.0	36.0	36.0	32.0	36.0
12	34.30918364327743	36.0	36.0	36.0	32.0	36.0
13	34.40331672657411	36.0	36.0	36.0	32.0	36.0
14	34.29296731124563	36.0	36.0	36.0	32.0	36.0
15	34.238958783744486	36.0	36.0	36.0	32.0	36.0
16	34.22073964186128	36.0	36.0	36.0	32.0	36.0
17	34.153271579179346	36.0	36.0	36.0	32.0	36.0
18	34.140959859119725	36.0	36.0	36.0	32.0	36.0
19	34.166665816433245	36.0	36.0	36.0	32.0	36.0
20	34.14009670214861	36.0	36.0	36.0	32.0	36.0
21	34.132700691647884	36.0	36.0	36.0	32.0	36.0
22	34.10164234488936	36.0	36.0	36.0	32.0	36.0
23	34.055753206485306	36.0	36.0	36.0	32.0	36.0
24	34.03526088052214	36.0	36.0	36.0	32.0	36.0
25	33.65094687095395	36.0	36.0	36.0	27.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
3	1.0
4	13.0
5	4.0
6	49.0
7	11.0
8	58.0
9	58.0
10	97.0
11	28.0
12	67.0
13	65.0
14	80.0
15	153.0
16	268.0
17	330.0
18	462.0
19	635.0
20	891.0
21	1336.0
22	2195.0
23	3183.0
24	5048.0
25	7439.0
26	10623.0
27	14046.0
28	19497.0
29	26497.0
30	34622.0
31	47566.0
32	67063.0
33	94865.0
34	203071.0
35	439802.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.82264992160962	16.874550888424352	11.050186177162267	26.25261301280376
2	16.96904395311749	19.707632233306498	37.03012141006686	26.29320240350915
3	18.722094798103058	23.75880518802086	28.686343490078603	28.832756523797475
4	12.538070910610266	15.22438497304809	35.99271079168108	36.24483332466056
5	14.654113335074772	36.556663455887154	33.7068585515575	15.082364657480571
6	34.27133801638387	35.45798519981878	16.948472838442918	13.322203945354433
7	30.350638893679644	30.31624752397446	21.058131619955976	18.274981962389923
8	28.380012798961385	32.49619552403786	19.581390269319137	19.542401407681623
9	27.76567553000359	13.820211184790775	18.31431908012936	40.09979420507627
10	15.675691127211918	26.418777302830048	32.003842686695464	25.90168888326257
11	37.33868950697	20.842525432466783	22.696836828968255	19.12194823159496
12	24.74174703358769	23.498110194738768	28.984875432123218	22.775267339550325
13	29.526279250214397	19.228468166782374	25.371115285661777	25.87413729734145
14	23.767294127016054	19.439971984305494	25.1979935698672	31.594740318811255
15	25.370767956991223	26.98819116690709	22.167773840479175	25.473267035622516
16	25.911341214522654	25.37757934705037	23.691451336089266	25.01962810233771
17	24.15442118600028	25.580590457968892	25.201408506784745	25.063579849246075
18	24.94054699824883	24.625540539850615	26.014223792432695	24.41968866946786
19	25.681912397460426	24.794104012955163	25.318212171398578	24.20577141818583
20	25.89914246795232	24.18683538202961	24.7488568621965	25.165165287821573
21	26.984922775940973	23.88493278249629	24.361877883266384	24.768266558296354
22	25.971337986102956	24.1584936871072	24.74419127069623	25.125977056093614
23	24.61446873168331	23.94633707002363	25.27392070646524	26.16527349182782
24	24.877722025147907	24.82503272658951	25.102468432370834	25.194776815891746
25	25.107902429859593	24.225690938033832	25.199391029011892	25.467015603094683
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	183.0
1	183.0
2	275.5
3	368.0
4	368.0
5	368.0
6	1100.0
7	1832.0
8	1832.0
9	1832.0
10	1814.5
11	1797.0
12	1797.0
13	1797.0
14	2081.5
15	2366.0
16	2366.0
17	2366.0
18	4096.5
19	5827.0
20	5827.0
21	5827.0
22	9451.5
23	13076.0
24	13076.0
25	13076.0
26	19602.5
27	26129.0
28	26129.0
29	26129.0
30	32469.5
31	38810.0
32	38810.0
33	38810.0
34	49342.5
35	59875.0
36	59875.0
37	59875.0
38	69050.0
39	78225.0
40	78225.0
41	78225.0
42	90215.0
43	102205.0
44	102205.0
45	102205.0
46	115370.0
47	128535.0
48	128535.0
49	128535.0
50	132173.5
51	135812.0
52	135812.0
53	135812.0
54	126700.0
55	117588.0
56	117588.0
57	117588.0
58	107961.0
59	98334.0
60	98334.0
61	98334.0
62	85872.0
63	73410.0
64	73410.0
65	73410.0
66	60239.0
67	47068.0
68	47068.0
69	47068.0
70	35296.5
71	23525.0
72	23525.0
73	23525.0
74	17863.0
75	12201.0
76	12201.0
77	12201.0
78	9774.5
79	7348.0
80	7348.0
81	7348.0
82	5024.0
83	2700.0
84	2700.0
85	2700.0
86	2083.0
87	1466.0
88	1466.0
89	1466.0
90	1051.5
91	637.0
92	637.0
93	637.0
94	455.5
95	274.0
96	274.0
97	274.0
98	403.0
99	532.0
100	532.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.04193351242650157
2	0.005713568603124302
3	0.0019385322046314595
4	0.004489232473883381
5	0.00928454898007699
6	0.013773781453960372
7	0.023058330434037363
8	0.03632197183414734
9	0.051728201460428946
10	0.06121680646204609
11	0.05795191011740363
12	0.06703240307594047
13	0.0656040109251594
14	0.06856282323749162
15	0.06142086248358624
16	0.0673384871082507
17	0.06591009495746962
18	0.07937779237911977
19	0.07601086802370723
20	0.08172443662683153
21	0.07805142823910877
22	0.07937779237911977
23	0.08417310888531337
24	0.08131632458375122
25	0.07815345624987884
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	980123.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	56.66250406355326
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.78910932800815	45.21050731525557
2	12.133395961287368	13.750171959222923
3	3.4485814965163537	5.862157891795573
4	1.4662117132139127	3.3231690863205086
5	0.7905787741450901	2.239808650127756
6	0.47194567654912156	1.6044974289144598
7	0.32640805341318335	1.2946568357030706
8	0.24233353143465677	1.0984979767721161
9	0.18053067322387673	0.9206388004629527
>10	0.9902395753168894	10.021868718197956
>50	0.07771760575217163	3.0393784449235652
>100	0.07571906130769766	8.51266678521378
>500	0.006686408594301171	2.6103026301266516
>1k	5.421412373757706E-4	0.5116774769630816
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	2185	0.22293120353261783	No Hit
TATCAACGCAGAGTACTTTTTTTTT	1551	0.15824544470438914	No Hit
GTACTTTTTTTTTTTTTTTTTTTTT	1261	0.12865732158106685	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.0202801077007681E-4
2	0.0	0.0	0.0	0.0	1.0202801077007681E-4
3	0.0	0.0	0.0	0.0	1.0202801077007681E-4
4	0.0	0.0	0.0	0.0	1.0202801077007681E-4
5	0.0	0.0	0.0	0.0	1.0202801077007681E-4
6	0.0	0.0	0.0	0.0	3.060840323102304E-4
7	0.0	0.0	0.0	0.0	3.060840323102304E-4
8	0.0	0.0	0.0	0.0	3.060840323102304E-4
9	0.0	0.0	0.0	0.0	3.060840323102304E-4
10	0.0	0.0	0.0	0.0	3.060840323102304E-4
11	0.0	0.0	0.0	0.0	3.060840323102304E-4
12	0.0	0.0	0.0	0.0	8.162240861606145E-4
13	0.0	0.0	0.0	0.0	0.0012243361292409217
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AATCGTA	40	2.7613936E-4	16.623749	13
CGTTATT	60	1.4697343E-6	15.828909	2
GTCCTAA	85	1.7156708E-8	14.528319	1
GCGTTAT	75	9.670384E-7	13.932285	1
ACGAACG	65	5.439273E-5	13.156215	15
GTATAAT	75	1.4784044E-5	12.665714	1
TGGATAG	60	4.0957468E-4	12.664421	5
CCTATTG	60	4.0992763E-4	12.663127	3
AGCGTAT	130	1.9645086E-10	12.424046	8
CGAACGA	70	1.08827524E-4	12.216485	16
CGATAAC	70	1.08983295E-4	12.214614	10
GATAACG	70	1.0903526E-4	12.213989	11
TCCAATA	180	0.0	12.135497	2
CCGGCGT	55	0.0030629176	12.093086	16
CGTAAAT	55	0.0030629176	12.093086	16
CTATGGT	80	2.8690281E-5	11.872894	4
ATCGTAA	65	8.010098E-4	11.693816	14
CGCGTAA	65	8.016965E-4	11.692622	10
TCGCGTA	65	8.016965E-4	11.692622	9
GTATCAA	1360	0.0	11.664564	1
>>END_MODULE