FastQCFastQC Report
Thu 2 Feb 2017
SRR4063986_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4063986_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences980123
Sequences flagged as poor quality0
Sequence length50
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA18250.022.299381
GTCTATA1057.7496225E-820.950511
TATACCG654.935915E-420.3048445
TAACGAT550.004481362620.00127625
GTAATAC1001.1245047E-619.7972223
TAGGACG901.0028563E-519.5528134
GTATATG1851.8189894E-1219.0253281
AACGCAG22000.018.7973616
GGCGTTA2450.017.95941242
CGCGGGA2350.017.78836844
GGGTATA750.001291406517.5984291
GTATTAA1903.274181E-1117.3668691
AAATCGC1602.1300366E-816.49852614
GAGCGAA4050.016.29484216
TCAACGC25500.016.1310714
AGAGCGA4100.016.09612315
CGTTATT2750.016.0010244
AGTAATA1105.626252E-515.9977552
CAACGCA25850.015.9126625
GCGAAAG4150.015.90138318
Produced by FastQC (version 0.11.5)