##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4063986_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	980123
Sequences flagged as poor quality	0
Sequence length	50
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.345047509343214	32.0	32.0	32.0	32.0	32.0
2	31.432772213283435	32.0	32.0	32.0	32.0	32.0
3	31.4981252353021	32.0	32.0	32.0	32.0	32.0
4	31.575644077324988	32.0	32.0	32.0	32.0	32.0
5	31.527902110245346	32.0	32.0	32.0	32.0	32.0
6	35.12827369626057	36.0	36.0	36.0	36.0	36.0
7	35.140039566462576	36.0	36.0	36.0	36.0	36.0
8	35.08665545038735	36.0	36.0	36.0	36.0	36.0
9	35.20061461673688	36.0	36.0	36.0	36.0	36.0
10	35.049324421526684	36.0	36.0	36.0	36.0	36.0
11	35.19824450604669	36.0	36.0	36.0	36.0	36.0
12	35.099894605064875	36.0	36.0	36.0	36.0	36.0
13	35.14139755928593	36.0	36.0	36.0	36.0	36.0
14	35.089582634016345	36.0	36.0	36.0	36.0	36.0
15	35.05564199595357	36.0	36.0	36.0	36.0	36.0
16	35.06808431186698	36.0	36.0	36.0	36.0	36.0
17	35.03064003191436	36.0	36.0	36.0	36.0	36.0
18	35.030514537461116	36.0	36.0	36.0	36.0	36.0
19	35.02799444559509	36.0	36.0	36.0	36.0	36.0
20	35.026205894566296	36.0	36.0	36.0	36.0	36.0
21	35.01599799208875	36.0	36.0	36.0	36.0	36.0
22	34.99780639776844	36.0	36.0	36.0	36.0	36.0
23	34.95677175211683	36.0	36.0	36.0	36.0	36.0
24	34.92631435034174	36.0	36.0	36.0	36.0	36.0
25	34.89651809007645	36.0	36.0	36.0	32.0	36.0
26	34.84002824135338	36.0	36.0	36.0	32.0	36.0
27	34.83306584989843	36.0	36.0	36.0	32.0	36.0
28	34.81398865244464	36.0	36.0	36.0	32.0	36.0
29	34.78234262434409	36.0	36.0	36.0	32.0	36.0
30	34.765957946094524	36.0	36.0	36.0	32.0	36.0
31	34.76109223026089	36.0	36.0	36.0	32.0	36.0
32	34.72045141273085	36.0	36.0	36.0	32.0	36.0
33	34.69077860635859	36.0	36.0	36.0	32.0	36.0
34	34.679827939962635	36.0	36.0	36.0	32.0	36.0
35	34.63632829757081	36.0	36.0	36.0	32.0	36.0
36	34.61779694997465	36.0	36.0	36.0	32.0	36.0
37	34.609361274044176	36.0	36.0	36.0	32.0	36.0
38	34.5608459346429	36.0	36.0	36.0	32.0	36.0
39	34.56750530290586	36.0	36.0	36.0	32.0	36.0
40	34.547042565065816	36.0	36.0	36.0	32.0	36.0
41	34.52997429914409	36.0	36.0	36.0	32.0	36.0
42	34.464940624799134	36.0	36.0	36.0	32.0	36.0
43	34.44957010497662	36.0	36.0	36.0	32.0	36.0
44	34.39775619998714	36.0	36.0	36.0	32.0	36.0
45	34.37378675941693	36.0	36.0	36.0	32.0	36.0
46	34.34180812000127	36.0	36.0	36.0	32.0	36.0
47	34.31281584046084	36.0	36.0	36.0	32.0	36.0
48	34.272054629878085	36.0	36.0	36.0	32.0	36.0
49	34.25745748237721	36.0	36.0	36.0	32.0	36.0
50	33.6567298186044	36.0	36.0	36.0	27.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	1.0
20	10.0
21	29.0
22	94.0
23	285.0
24	751.0
25	1757.0
26	3555.0
27	6549.0
28	11138.0
29	16719.0
30	24014.0
31	33887.0
32	49031.0
33	79103.0
34	166864.0
35	586336.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.847280979406584	17.81817142880466	11.565148537481813	26.76939905430694
2	16.11807569912572	20.544077900903666	36.56124727966151	26.776599120309108
3	17.926460100334552	24.3067947732984	29.014478824553297	28.752266301813755
4	12.224282054395214	15.945855775244535	36.02425409872026	35.80560807163999
5	14.241273799308862	37.029332032816285	33.462024664251324	15.267369503623524
6	34.28467942700893	35.885299759213154	16.601844672080972	13.228176141696935
7	30.233552319453782	30.60830120301227	20.98471314314632	18.173433334387624
8	28.19503266426765	32.826594213175284	19.382873374056114	19.595499748500952
9	27.726462518888244	13.613921745278832	18.204338914016528	40.45527682181639
10	15.520047178233531	26.689473560701675	32.08395358494378	25.706525676121018
11	37.56099999693916	20.765659004022964	22.64593321450471	19.027407784533164
12	24.682870788402656	23.609431896673982	28.82827732494253	22.87941998998083
13	29.554124660627252	19.31830587572708	25.354524000706036	25.77304546293963
14	23.685340891583795	19.296801109250797	25.304834811212086	31.713023187953326
15	25.3236264941611	27.062694821261857	22.123124486641192	25.49055419793585
16	25.99161731876635	25.342510478351542	23.64138530541283	25.024486897469277
17	24.13380769556474	25.657596036415836	25.11950030761445	25.08909596040497
18	25.03287866065243	24.561201241890014	26.069793565883327	24.336126531574227
19	25.562762148755102	24.702154121787004	25.408699538118253	24.32638419133964
20	25.96536550079021	23.945716906381968	24.947429960178386	25.14148763264944
21	26.981715560189894	23.966787841934124	24.378368837380616	24.673127760495365
22	25.909911307050237	24.149111897180255	24.881162874455555	25.059813921313957
23	24.61749425578197	23.806671863330465	25.39740194017851	26.178431940709057
24	24.91360742930195	24.73383334064537	25.159087825049813	25.19347140500286
25	25.000586669441866	24.173637830078082	25.4204379308832	25.405337569596853
26	25.01928437618614	24.824501973332463	25.510474783382374	24.645738867099016
27	25.470199364734714	24.568726704303703	24.72381877491549	25.23725515604609
28	24.772519962533902	24.351536497817538	25.64703505610732	25.22890848354124
29	24.866952351800837	24.643505764717887	25.274461789613305	25.215080093867975
30	24.776243668910013	24.765020141130762	25.647699584933537	24.81103660502569
31	25.297102491814183	24.511019346991834	24.695397883167068	25.49648027802692
32	25.086369266621368	24.69334574030654	24.58876393488788	25.631521058184216
33	24.794047844976237	24.307864035866388	24.997602241445104	25.90048587771227
34	25.272191335382782	24.421666746590404	25.347081576286524	24.95906034174029
35	26.031962077300353	24.15929411212541	25.201739824242246	24.607003986331993
36	25.089358141717984	24.81579927452311	24.80508548076874	25.28975710299017
37	25.90193707688619	24.558176076564788	24.686736354499864	24.85315049204916
38	24.957526940419353	24.43795259955532	25.201090167371916	25.40343029265341
39	25.460932701274796	24.526313211805594	24.546515685486256	25.466238401433355
40	25.712606583620634	24.581598389550443	25.078588999682683	24.627206027146237
41	24.808359240844453	24.876312253274957	25.31708855012147	24.998239955759118
42	25.94130220741655	25.036603221083453	24.759592084521557	24.262502486978434
43	25.147916617777742	24.211698038899595	25.154956622348646	25.485428720974014
44	24.951918613133845	24.82693251464888	24.641851059422983	25.579297812794294
45	25.13319933476855	24.915162583791613	24.82782544816398	25.123812633275854
46	24.91335182779945	24.731127941370026	24.83111641208718	25.524403818743345
47	25.18357689517573	24.692099944189767	25.087668999405167	25.036654161229333
48	25.792387973966107	25.023696392332674	24.30520341146426	24.87871222223696
49	25.00617272614114	25.158501323310052	24.504090323818154	25.33123562673066
50	25.10304986797373	25.18395931876895	24.60575773282128	25.107233080436032
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	72.0
1	51.5
2	31.0
3	111.0
4	191.0
5	216.5
6	242.0
7	204.5
8	167.0
9	253.0
10	339.0
11	501.5
12	664.0
13	1115.5
14	1567.0
15	1975.5
16	2384.0
17	2540.5
18	2697.0
19	2724.0
20	2751.0
21	3024.0
22	3297.0
23	3664.5
24	4032.0
25	4706.0
26	5380.0
27	6513.0
28	7646.0
29	9166.0
30	10686.0
31	12216.0
32	13746.0
33	15973.0
34	18200.0
35	21040.0
36	23880.0
37	29026.0
38	34172.0
39	37839.0
40	41506.0
41	45694.0
42	49882.0
43	51427.0
44	52972.0
45	58583.0
46	64194.0
47	68631.0
48	73068.0
49	75476.0
50	77884.0
51	75846.5
52	73809.0
53	73674.0
54	73539.0
55	73313.0
56	73087.0
57	70833.0
58	68579.0
59	62599.0
60	56619.0
61	49781.0
62	42943.0
63	37410.5
64	31878.0
65	26805.0
66	21732.0
67	18612.0
68	15492.0
69	13754.0
70	12016.0
71	9337.0
72	6658.0
73	5663.0
74	4668.0
75	3638.5
76	2609.0
77	2274.5
78	1940.0
79	1583.0
80	1226.0
81	969.0
82	712.0
83	605.0
84	498.0
85	365.0
86	232.0
87	160.5
88	89.0
89	68.5
90	48.0
91	45.5
92	43.0
93	29.5
94	16.0
95	19.0
96	22.0
97	16.0
98	10.0
99	9.0
100	8.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.010304829087777759
2	6.121680646204608E-4
3	2.0405602154015363E-4
4	0.0
5	0.0
6	3.060840323102304E-4
7	0.0
8	0.0
9	0.001632448172321229
10	0.0010202801077007682
11	0.0
12	2.0405602154015363E-4
13	6.121680646204608E-4
14	2.0405602154015363E-4
15	0.005917624624664455
16	7.141960753905377E-4
17	0.0
18	4.0811204308030725E-4
19	2.0405602154015363E-4
20	2.0405602154015363E-4
21	0.0
22	0.0
23	7.141960753905377E-4
24	4.0811204308030725E-4
25	0.0014283921507810755
26	0.005611540592354225
27	0.006121680646204609
28	0.0037750363984928423
29	0.0023466442477117666
30	0.003979092420032995
31	0.007550072796985685
32	0.0029588123123322277
33	0.004183148441573149
34	0.0022446162369416903
35	0.0024486722584818434
36	0.00795818484006599
37	0.003877064409262919
38	0.009182520969306914
39	0.004591260484653457
40	0.0024486722584818434
41	0.003468952366182612
42	0.0018365041938613828
43	0.0010202801077007682
44	0.0014283921507810755
45	0.0013263641400109987
46	0.0010202801077007682
47	0.001632448172321229
48	0.001632448172321229
49	5.101400538503841E-4
50	0.0015304201615511522
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	980123.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	59.75531636198279
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.7757961431149	48.26783252922861
2	11.781530015037921	14.080181065535738
3	3.2433386260750736	5.814201770104639
4	1.3443959707677315	3.2133922619600312
5	0.6964280584391397	2.080763947769612
6	0.4545791574410425	1.6298112818671848
7	0.3013533420997201	1.2605225005736722
8	0.22767311051445693	1.0883742996726435
9	0.17378911874722047	0.9346341393909318
>10	0.8657100477036753	9.255320609006683
>50	0.06719420099434639	2.8047884927546893
>100	0.06340127259929008	7.56470285122465
>500	0.004810936465434864	2.005474250910934
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.0202801077007681E-4
2	0.0	0.0	0.0	0.0	1.0202801077007681E-4
3	0.0	0.0	0.0	0.0	1.0202801077007681E-4
4	0.0	0.0	0.0	0.0	1.0202801077007681E-4
5	0.0	0.0	0.0	0.0	1.0202801077007681E-4
6	0.0	0.0	0.0	0.0	2.0405602154015363E-4
7	0.0	0.0	0.0	0.0	2.0405602154015363E-4
8	0.0	0.0	0.0	0.0	2.0405602154015363E-4
9	0.0	0.0	0.0	0.0	2.0405602154015363E-4
10	0.0	0.0	0.0	0.0	2.0405602154015363E-4
11	0.0	0.0	0.0	0.0	2.0405602154015363E-4
12	0.0	0.0	0.0	0.0	7.141960753905377E-4
13	0.0	0.0	0.0	0.0	0.0010202801077007682
14	0.0	0.0	0.0	0.0	0.0010202801077007682
15	0.0	0.0	0.0	0.0	0.0012243361292409217
16	0.0	0.0	0.0	0.0	0.0012243361292409217
17	0.0	0.0	0.0	0.0	0.0012243361292409217
18	0.0	0.0	0.0	0.0	0.0013263641400109987
19	0.0	0.0	0.0	0.0	0.0014283921507810755
20	0.0	0.0	0.0	0.0	0.0015304201615511522
21	0.0	0.0	0.0	3.060840323102304E-4	0.001734476183091306
22	0.0	0.0	0.0	3.060840323102304E-4	0.001734476183091306
23	0.0	0.0	0.0	0.0012243361292409217	0.001734476183091306
24	0.0	0.0	0.0	0.002142588226171613	0.001734476183091306
25	0.0	0.0	0.0	0.0024486722584818434	0.001734476183091306
26	0.0	0.0	0.0	0.0029588123123322277	0.0018365041938613828
27	0.0	0.0	0.0	0.0033669243554125348	0.0018365041938613828
28	0.0	0.0	0.0	0.005101400538503841	0.0018365041938613828
29	0.0	0.0	0.0	0.009488605001617145	0.0019385322046314595
30	0.0	0.0	0.0	0.015610285647821753	0.0019385322046314595
31	0.0	0.0	0.0	0.03417938360797573	0.002142588226171613
32	0.0	0.0	0.0	0.051728201460428946	0.002142588226171613
33	0.0	0.0	0.0	0.07203177560367423	0.002142588226171613
34	0.0	0.0	0.0	0.09335562985462029	0.00224461623694169
35	0.0	0.0	0.0	0.12029102469792056	0.00224461623694169
36	0.0	0.0	0.0	0.16140831303826153	0.00224461623694169
37	0.0	0.0	0.0	0.2174216909510337	0.00224461623694169
38	0.0	0.0	0.0	0.29832990349170463	0.00224461623694169
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATCAA	1825	0.0	22.29938	1
GTCTATA	105	7.7496225E-8	20.95051	1
TATACCG	65	4.935915E-4	20.304844	5
TAACGAT	55	0.0044813626	20.001276	25
GTAATAC	100	1.1245047E-6	19.797222	3
TAGGACG	90	1.0028563E-5	19.552813	4
GTATATG	185	1.8189894E-12	19.025328	1
AACGCAG	2200	0.0	18.797361	6
GGCGTTA	245	0.0	17.959412	42
CGCGGGA	235	0.0	17.788368	44
GGGTATA	75	0.0012914065	17.598429	1
GTATTAA	190	3.274181E-11	17.366869	1
AAATCGC	160	2.1300366E-8	16.498526	14
GAGCGAA	405	0.0	16.294842	16
TCAACGC	2550	0.0	16.131071	4
AGAGCGA	410	0.0	16.096123	15
CGTTATT	275	0.0	16.00102	44
AGTAATA	110	5.626252E-5	15.997755	2
CAACGCA	2585	0.0	15.912662	5
GCGAAAG	415	0.0	15.901383	18
>>END_MODULE