FastQCFastQC Report
Thu 2 Feb 2017
SRR4063985_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4063985_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences866378
Sequences flagged as poor quality0
Sequence length25
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT31040.35827317868182246No Hit
TATCAACGCAGAGTACTTTTTTTTT22520.25993273144054907No Hit
GTACTTTTTTTTTTTTTTTTTTTTT20710.23904115755478558No Hit
GAGTACTTTTTTTTTTTTTTTTTTT14190.16378532234198007No Hit
GTACATGGAAGCAGTGGTATCAACG13380.15443605447045056No Hit
GTACATGGGGTGGTATCAACGCAAA12270.14162409479465085No Hit
TCCATGTACTCTGCGTTGATACCAC11750.13562209566724917No Hit
ACGCAGAGTACTTTTTTTTTTTTTT11660.1345832881259681No Hit
GGTATCAACGCAGAGTACTTTTTTT10800.12465690495372689No Hit
GTTCAAAGCAGGCCCGAGCCGCCTG10390.11992455948789096No Hit
GATTAAGAGGGACGGCCGGGGGCAT9980.11519221402205504No Hit
GTATCTGATCGTCTTCGAACCTCCG9640.11126782997721547No Hit
CTATTGGAGCTGGAATTACCGCGGC9360.10803598429322998No Hit
GAATAGGACCGCGGTTCTATTTTGT9290.10722802287223358No Hit
GTCTTGCGCCGGTCCAAGAATTTCA9040.10434244636867511No Hit
GTCCTATTCCATTATTCCTAGCTGC8850.10214940822597066No Hit
GCGCAAGACGGACCAGAGCGAAAGC8700.10041806232383556No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATTACAC504.5690904E-617.0954783
CTTAGTC453.5439953E-516.8844223
CGCGCTA350.0021696416.2851716
TAACGAA851.7138518E-814.52892613
TCTATCC400.00528586914.2462333
CGAACGA954.8912625E-913.99953116
CGAGACT759.655814E-713.9336724
GTATTAC759.687319E-713.9296481
AATTCCG1101.8553692E-1013.8185185
CACGCGC500.001498989213.29955512
ATAACGA1001.0051735E-813.29955512
ACGGTAT1450.013.1052819
ACGAACG957.3750925E-812.99956415
AGGCCCG1700.012.85473910
CGGTCGG1051.9848812E-812.66843810
CAATACG905.394104E-712.6669754
CGCCCCT905.394104E-712.6669755
TCGGCGT1051.9896106E-812.66624413
GTCCTAT2000.012.3467351
GACGGTA1551.8189894E-1212.2604878
Produced by FastQC (version 0.11.5)