##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4063985_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	866378
Sequences flagged as poor quality	0
Sequence length	25
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.219783974200638	32.0	32.0	32.0	32.0	32.0
2	30.83203751711147	32.0	32.0	32.0	32.0	32.0
3	30.872553319682634	32.0	32.0	32.0	32.0	32.0
4	30.89455988032937	32.0	32.0	32.0	32.0	32.0
5	30.81433508237744	32.0	32.0	32.0	32.0	32.0
6	34.45393465669719	36.0	36.0	36.0	32.0	36.0
7	34.37181692055893	36.0	36.0	36.0	32.0	36.0
8	34.33049431079736	36.0	36.0	36.0	32.0	36.0
9	34.463124640745725	36.0	36.0	36.0	32.0	36.0
10	34.17533339950922	36.0	36.0	36.0	32.0	36.0
11	34.45932837629764	36.0	36.0	36.0	32.0	36.0
12	34.262985671381315	36.0	36.0	36.0	32.0	36.0
13	34.36118991941162	36.0	36.0	36.0	32.0	36.0
14	34.25823370399525	36.0	36.0	36.0	32.0	36.0
15	34.205880112375894	36.0	36.0	36.0	32.0	36.0
16	34.194441687115784	36.0	36.0	36.0	32.0	36.0
17	34.12120575545547	36.0	36.0	36.0	32.0	36.0
18	34.1153907416855	36.0	36.0	36.0	32.0	36.0
19	34.11964523568235	36.0	36.0	36.0	32.0	36.0
20	34.09580806530175	36.0	36.0	36.0	32.0	36.0
21	34.06024044931889	36.0	36.0	36.0	32.0	36.0
22	34.05850564072495	36.0	36.0	36.0	32.0	36.0
23	33.98925065040894	36.0	36.0	36.0	32.0	36.0
24	33.97212879366743	36.0	36.0	36.0	32.0	36.0
25	33.57090554007604	36.0	36.0	36.0	27.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
3	1.0
4	8.0
5	11.0
6	39.0
7	14.0
8	50.0
9	43.0
10	74.0
11	27.0
12	57.0
13	64.0
14	93.0
15	190.0
16	271.0
17	379.0
18	483.0
19	638.0
20	1010.0
21	1407.0
22	2149.0
23	3249.0
24	4722.0
25	7002.0
26	9771.0
27	13101.0
28	17455.0
29	23695.0
30	30622.0
31	41828.0
32	58837.0
33	83109.0
34	177529.0
35	388450.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	46.31574693132874	16.805695084352372	11.019387766884908	25.85917021743398
2	16.590367620577886	19.6904636581064	37.56614123057318	26.153027490742538
3	18.7114624140948	24.513309678864033	28.42783963285555	28.34738827418562
4	12.23236577744791	15.28739838008359	35.67775275555468	36.80248308691381
5	14.197852227825921	37.1646775398162	33.508870555343286	15.12859967701459
6	34.164147212323336	35.36771335131872	17.041669985535503	13.426469450822445
7	30.20785230064466	30.39141437464211	21.01878105547038	18.381952269242845
8	28.734545236214835	32.50499956123943	19.345184487273634	19.4152707152721
9	27.914324382999755	13.553103824177448	18.296257119233594	40.2363146735892
10	16.088465425147973	26.42991193879024	31.537924065251914	25.943698570809875
11	37.42725583425822	20.587335152683348	22.726207070981214	19.259201942077222
12	25.342389810423803	23.42445248814427	28.854873843012335	22.37828385841959
13	29.94663956295261	19.317517700188265	25.294233145840312	25.441609591018814
14	23.75721428431181	19.754405376655807	25.1485013576772	31.339878981355184
15	25.17996495981466	27.399253692558755	22.570314241398464	24.850467106228123
16	25.58267391859255	25.275672129481297	24.407104238542008	24.734549713384148
17	23.769853736059652	25.724851934324445	25.593648652394457	24.911645677221447
18	24.866957836579058	24.302676553044172	27.08480853133234	23.74555707904443
19	25.664171691269893	24.704761244715502	25.462032480883405	24.169034583131193
20	26.051514143402365	23.829854084074924	25.225596739763244	24.893035032759464
21	26.959497858459663	23.85368682224984	24.194092398109348	24.99272292118115
22	25.973436402617082	24.458816951909217	24.78583706058478	24.781909584888922
23	24.223062927773125	24.31905738296705	25.746498397204494	25.711381292055336
24	25.018193327496068	24.940683976703298	25.36958445284867	24.67153824295196
25	24.946805684225602	24.425605352468654	25.369356839714722	25.25823212359101
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	165.0
1	165.0
2	348.5
3	532.0
4	532.0
5	532.0
6	1599.0
7	2666.0
8	2666.0
9	2666.0
10	2546.5
11	2427.0
12	2427.0
13	2427.0
14	2226.5
15	2026.0
16	2026.0
17	2026.0
18	3555.5
19	5085.0
20	5085.0
21	5085.0
22	8389.0
23	11693.0
24	11693.0
25	11693.0
26	18349.5
27	25006.0
28	25006.0
29	25006.0
30	31589.0
31	38172.0
32	38172.0
33	38172.0
34	45973.0
35	53774.0
36	53774.0
37	53774.0
38	60289.0
39	66804.0
40	66804.0
41	66804.0
42	77687.0
43	88570.0
44	88570.0
45	88570.0
46	102642.5
47	116715.0
48	116715.0
49	116715.0
50	117262.5
51	117810.0
52	117810.0
53	117810.0
54	108153.5
55	98497.0
56	98497.0
57	98497.0
58	90936.5
59	83376.0
60	83376.0
61	83376.0
62	73815.0
63	64254.0
64	64254.0
65	64254.0
66	53568.0
67	42882.0
68	42882.0
69	42882.0
70	32200.5
71	21519.0
72	21519.0
73	21519.0
74	16670.5
75	11822.0
76	11822.0
77	11822.0
78	9535.5
79	7249.0
80	7249.0
81	7249.0
82	4919.5
83	2590.0
84	2590.0
85	2590.0
86	1985.0
87	1380.0
88	1380.0
89	1380.0
90	992.5
91	605.0
92	605.0
93	605.0
94	431.0
95	257.0
96	257.0
97	257.0
98	379.5
99	502.0
100	502.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.042475686132381014
2	0.005771153007116986
3	0.0018467689622774354
4	0.004270653225266569
5	0.009810960112098876
6	0.013735344156938428
7	0.02146868918647519
8	0.034857764162986596
9	0.04778514689892864
10	0.05805779925159688
11	0.05782695313131219
12	0.06371352919857153
13	0.06556029816084896
14	0.06475233673985258
15	0.05944287597330495
16	0.06752249018326874
17	0.06302099083771749
18	0.07791056559607931
19	0.07363991237081274
20	0.07779514253593697
21	0.07456329685195145
22	0.07871852701707568
23	0.08114241128006482
24	0.07802598865622165
25	0.07894937313736036
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	866378.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	49.343750861913485
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.47028163043942	36.74643023388935
2	14.546163794254188	14.355245645205297
3	4.879019244087171	7.222473300921567
4	2.162109784049546	4.267464260809853
5	1.1189896089180642	2.760757223976148
6	0.6429475082301761	1.9035264998038728
7	0.40741432293728375	1.4072345594014743
8	0.2791134935916042	1.1018005351985922
9	0.2037059190279234	0.9046452705849877
>10	1.06462979627436	9.519823007452437
>50	0.09988676056168044	3.474495061321387
>100	0.11234173792391323	10.753891749325103
>500	0.011046154142448283	3.7416538694853214
>1k	0.002350245562223039	1.8405587826246448
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	3104	0.35827317868182246	No Hit
TATCAACGCAGAGTACTTTTTTTTT	2252	0.25993273144054907	No Hit
GTACTTTTTTTTTTTTTTTTTTTTT	2071	0.23904115755478558	No Hit
GAGTACTTTTTTTTTTTTTTTTTTT	1419	0.16378532234198007	No Hit
GTACATGGAAGCAGTGGTATCAACG	1338	0.15443605447045056	No Hit
GTACATGGGGTGGTATCAACGCAAA	1227	0.14162409479465085	No Hit
TCCATGTACTCTGCGTTGATACCAC	1175	0.13562209566724917	No Hit
ACGCAGAGTACTTTTTTTTTTTTTT	1166	0.1345832881259681	No Hit
GGTATCAACGCAGAGTACTTTTTTT	1080	0.12465690495372689	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTG	1039	0.11992455948789096	No Hit
GATTAAGAGGGACGGCCGGGGGCAT	998	0.11519221402205504	No Hit
GTATCTGATCGTCTTCGAACCTCCG	964	0.11126782997721547	No Hit
CTATTGGAGCTGGAATTACCGCGGC	936	0.10803598429322998	No Hit
GAATAGGACCGCGGTTCTATTTTGT	929	0.10722802287223358	No Hit
GTCTTGCGCCGGTCCAAGAATTTCA	904	0.10434244636867511	No Hit
GTCCTATTCCATTATTCCTAGCTGC	885	0.10214940822597066	No Hit
GCGCAAGACGGACCAGAGCGAAAGC	870	0.10041806232383556	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	1.1542306014233971E-4
13	0.0	0.0	0.0	0.0	1.1542306014233971E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATTACAC	50	4.5690904E-6	17.095478	3
CTTAGTC	45	3.5439953E-5	16.884422	3
CGCGCTA	35	0.00216964	16.28517	16
TAACGAA	85	1.7138518E-8	14.528926	13
TCTATCC	40	0.005285869	14.246233	3
CGAACGA	95	4.8912625E-9	13.999531	16
CGAGACT	75	9.655814E-7	13.933672	4
GTATTAC	75	9.687319E-7	13.929648	1
AATTCCG	110	1.8553692E-10	13.818518	5
CACGCGC	50	0.0014989892	13.299555	12
ATAACGA	100	1.0051735E-8	13.299555	12
ACGGTAT	145	0.0	13.105281	9
ACGAACG	95	7.3750925E-8	12.999564	15
AGGCCCG	170	0.0	12.854739	10
CGGTCGG	105	1.9848812E-8	12.668438	10
CAATACG	90	5.394104E-7	12.666975	4
CGCCCCT	90	5.394104E-7	12.666975	5
TCGGCGT	105	1.9896106E-8	12.666244	13
GTCCTAT	200	0.0	12.346735	1
GACGGTA	155	1.8189894E-12	12.260487	8
>>END_MODULE