FastQCFastQC Report
Thu 2 Feb 2017
SRR4063985_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4063985_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences866378
Sequences flagged as poor quality0
Sequence length50
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA10240.11819321358575587No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAA9720.1121912144583542No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT9430.10884394571422636No Hit
GTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA9160.10572752309038318No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA9160.10572752309038318No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATACGAATGCC8830.10191856210568598No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTCGTA300.005748066629.3292182
GTATTAT651.8145922E-523.6889821
CGAGCCG2750.023.19808215
TTACGGC500.00258109121.99818218
TGTAGGA5100.021.1342892
ATTAGCC550.00448624619.9971943
GTATATA1152.0227344E-719.127751
CTGTAGG5300.019.091661
TAGGACG5350.018.9143244
TAACGGC708.112693E-418.8599436
GTCTAGA1051.7939001E-618.8544961
ATTTCGT2000.018.70061342
GAGCCGC3650.018.68230816
TCGAACG2150.018.41814644
TTAACGG600.007404112718.33605235
CCTAATA600.00741644918.3307612
ACTAGTA851.4311454E-418.116158
GCTATAG851.4317781E-418.1151051
TATATAC851.4317781E-418.1151053
GTATTAG2100.017.8070241
Produced by FastQC (version 0.11.5)