FastQCFastQC Report
Thu 2 Feb 2017
SRR4063984_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4063984_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1424354
Sequences flagged as poor quality0
Sequence length25
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT35990.2526759499394111No Hit
TATCAACGCAGAGTACTTTTTTTTT25750.18078370966768093No Hit
GTACTTTTTTTTTTTTTTTTTTTTT17130.12026504647018929No Hit
GATTAAGAGGGACGGCCGGGGGCAT16380.11499950152841218No Hit
GTTCAAAGCAGGCCCGAGCCGCCTG15200.10671504415334951No Hit
GAATAGGACCGCGGTTCTATTTTGT14980.10517048430376157No Hit
CTATTGGAGCTGGAATTACCGCGGC14540.10208136460458565No Hit
GTATCTGATCGTCTTCGAACCTCCG14260.10011556115965554No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGTAA851.0186341E-915.64810110
CGTTATT1100.015.5388462
TCGCGTA902.2846507E-914.7787619
GCGTAAC902.2864697E-914.77824211
ATAATAC802.0030948E-613.0601583
CGTCGTA2700.013.01938510
TAGAGTG1800.012.665735
GCGTTAT1352.7284841E-1112.6635061
AACGTCT1155.322363E-912.3912596
CGAACGA1700.012.29536816
CGAACGT1251.4115358E-912.1586744
TCGAACG1251.4133548E-912.1578193
GCCGTTA550.003065891312.09213915
GCATCGC2200.012.09213914
CGCATCG2200.012.09128913
AATCGAA951.038281E-612.0012217
CGGTCCA3500.011.94365210
GTCGTAG2950.011.91562911
ACGTCTG1209.993528E-911.8762077
TATACTC802.8681336E-511.8741225
Produced by FastQC (version 0.11.5)